Molecular detection of Crimean-Congo hemorrhagic fever virus in tick species collected from livestock in the border line of Iran-Afghanistan
Sahar
Asadolahizoj
Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Dariush
Saadati
Department of Nutrition and Animal Breeding, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Mehdi
Rasekh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Faezeh
Faghihi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
author
Mehdi
Fazlalipour
Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran
author
Sahar
Khakifirouz
Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (I
author
Tahmineh
Jalali
Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran
author
zahra
ahmadi
Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran
author
text
article
2021
per
Crimean-Congo Hemorrhagic Fever (CCHF) is a viral, acute, febrile, and hemorrhagic disease that causes significant death in humans. Crimean-Congo hemorrhagic fever virus has been isolated from different species of ticks, including 28 hard ticks and 2 soft ticks. The aim of this study was to determine the prevalence of CCHF virus in ticks isolated from domestic livestock in border villages of Sistan region in the north of Sistan and Baluchestan province. In this study, 54 livestock including 50 sheep, 3 goats and 1 cow in four villages (Choto, Nader Alamkhan, Millak and Sanjarani) from Sistan region were sampled. After identifying genus and species of ticks, the samples were examined for the presence of the virus genome by reverse polymerase chain reaction (RT-PCR).Acording to taxonomic ranks 2 genera and 3 species were identified, which include Rhipicephalus Sanguineous, Rhipicephalus (Boophilus) annulatus and Hyalomma anatolicum. The presence of the virus was not confirmed in any of the 50 tested samples. Our findings indicate that CCHFV may not circulating in the ticks of Sistan border villages. However, since Sistan and Baluchestan province is an endemic region for CCHF, more research is needed for a better understanding of CCHFV vectors in this region.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
1
9
https://nfvm.uoz.ac.ir/article_128845_cdcb917d441d3e80bba77ed13930f1a6.pdf
dx.doi.org/10.22034/nfvm.2021.128845
Evaluation of antimicrobial activity of Zataria multiflora Bioss, Mentha piperita L, Cuminum cyminum L., Foeniculum vulgare Mill and Anethum graveolens L. essential oil on Escherichia coli isolated from poultry feces
Bahman
Fazeli-Nasab
Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran
author
Pantea
Ramazannezhad
Shahrekord University of Medical Sciences, Shahrekord, Iran
author
yasoub
shiri
Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran
author
text
article
2021
per
The aim of this study was to investigate the effect of thyme, peppermint, cumin, dill, and fennel essential oils on Escherichia coli isolated from poultry feces. The essential oil of the plants used was obtained with a Clevenger Apparatus. Escherichia coli strains were isolated from poultry feces, isolated, and finally the minimum inhibitory concentration and the minimum lethal concentration were determined by Fine-grained method. All strains of Escherichia coli at concentrations, 1.56 mg/ml were inhibited by both thyme and peppermint. The MBC (3.12 mg / ml) for Zataria multiflora and peppermint has inhibited one- and two-way, respectively. All Escherichia coli strains at concentrations of 0.63 mg/ml were inhibited by all three cumin, dill, and fennel plants. The MBC (1.25 mg / ml) for cumin has led to double-sided control, but the concentration for dill and fennel was 5 mg/ml. The results showed that cumin, thyme, peppermint and then fennel and dill essential oils, respectively, with emphasis on the effectiveness of cumin essential oil, can be useful alone or in combination with other antimicrobial agents to treat infections caused by Escherichia coli bacteria.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
10
23
https://nfvm.uoz.ac.ir/article_128846_2acc43af900d30c90d7a72b734ee38f7.pdf
dx.doi.org/10.22034/nfvm.2021.128846
A systematic review on the spread of Schmallenberg virus (SBV) in Iran and neighboring countries
Sahar
Asadolahizoj
Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Amirsajad
Jafari
Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Amir Masoud
Jafari-Nozad
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
author
Mehdi
Rasekh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Ali
Sarani
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Hasan
Bakhshi
Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran (IPI), Tehran, Iran
author
text
article
2021
per
Schmallenberg virus (SBV) is a single-stranded RNA virus classified in the Prebunyaviridae family and Orthobunyavirus genus. SBV is a teratogenic arbovirus, infecting pregnant ruminants, and results in offspring with congenital defects such as scoliosis, hydrocephalus, arthrogryposis, cerebellar hypoplasia and enlarged thymus. The purpose of this review was to conduct a systematic review to show the spread of SBV in Iran and neighboring countries including Afghanistan, Pakistan, Turkmenistan, the Republic of Azerbaijan, Armenia, Turkey, Iraq, Russia, Kazakhstan, United Arab Emirates, Bahrain, Saudi Arabia, Oman and Qatar. In this study, 9 English and Persian databases including Magiran, Irandoc, SID, Web of Science, Scopus, Science Direct, Google Scholar and PubMed were reviewed. Of the 55 studies found, 12 studies met the criteria defined in the purpose of this study. The results of the study indicated the presence of SBV in a number of domestic animals such as horses, cows, sheep, goats and camels. In Iran, only one study was found to be conducted on the prevalence of the disease which indicates that horses are infected (5%). Turkey and Pakistan showed the highest percentages of positive serum samples in cattle (39.82%) and camels (86%) respectively. Due to the proximity and livestock exchanges between Iran and Turkey in the northwest of the country, as well as between Iran and Pakistan in the southeast, precautionary measures are recommended by veterinary officials.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
24
34
https://nfvm.uoz.ac.ir/article_128913_0c2a85c2e52a50130642d34e73e69d37.pdf
dx.doi.org/10.22034/nfvm.2021.128913
A review on viral agents of cattle abortion in Iran
Mohammad Javad
Behzadi Shahrbabak
Assistant professor, Department of clinical science, Faculty of Veterinary Medicine, University of Zabol, Zabol-Iran
author
text
article
2021
per
pregnancy wastage is a serious economic problem in the reproductive management of dairy and beef farms. Various agents including genetic disorders, nutritional deficiencies, poisonings, physical injuries and infections cause pregnancy loss in cows. Infectious agents including bacteria, viruses, protozoa and fungi have a significant effect on pregnancy loss of cows. Diagnosis of infectious abortive agents in each region is necessary to control and prevent abortions on farms. The purpose of this study is to review all studies that have investigated viral agents of cattle pregnancy loss in Iran. Therefore, all Persian and English articles on the subject of cattle abortion with viral origin or any of the viruses causing abortion were searched in databases including Scince Direct, Pub Med, Scopus, Google Scholar, Magiran and Iran Doc. All the studies found were placed in the tables for easier analysis. The data of present study indicate that although many studies show a high prevalence of viral infections causing pregnancy loss of cattle including BVD and IBR viruses, but studies investigate their role in pregnancy loss of cattle in Iran are few. These few studies indicate that BVD and IBR viruses have a significant role in causing abortion in cattle in Iran. Only one study has examined the role of Blue tongue virus in bovine abortion in Iran, which no trace of this virus has been found.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
35
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https://nfvm.uoz.ac.ir/article_129025_12b16c4347c66576497b2fc65097fca1.pdf
dx.doi.org/10.22034/nfvm.2021.129025
Seroprevalence of toxoplasmosis in Kermanshah, A cross-sectional study
mehrdad
pooyanmehr
Assistant Professor of Immunology, Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University
author
Atefeh
Nik Ghalb
graduate student in Veterinary Medicine, Faculty of Veterinary Medicine, Razi University, Iran
author
Masoud
Darabi
Undergraduate student of Laboratory Sciences, Faculty of Veterinary Medicine, Razi University, Iran
author
text
article
2021
per
Toxoplasmosis is a common infectious disease (zoonosis) with worldwide spread. It is caused by Toxoplasma gondii, an intracellular protozoan with global distribution. The present study was a cross-sectional study of the prevalence of Toxoplasmosis and its associated risk factors with the aim of investigating more information related to T. gondii infection in Kermanshah. In this study, 2470 serum samples were collected from suspects referred to the reference laboratory during a period of one year (2019-2020) and evaluated anti T. gondii, IgG IgM antibodies by using ELISA&DAT method. Multivariate data analysis was performed using Chi-Square Pearson and Fisher statistical tests by SPSS ver.18 software with significant P levels less than 0.05. The overall prevalence of anti-T. gondii antibodies in the study population was 25.4% (P
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
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2
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2021
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https://nfvm.uoz.ac.ir/article_133112_bf8f6dd71f278a8c70d13690fd455c78.pdf
dx.doi.org/10.22034/nfvm.2021.133112
Effect of Phytogenic Additiv on Some Blood Parameters, Microbial Pop-ulation and Antibody Response of Broiler Chickens under Heat Stress Condition
Ahmad Ali
Sabetan Shirazi
Assistant Professor, Department of Agriculture, Fasa Branch, Islamic Azad University, Fasa, Iran
author
Masood
Abedi
Instructor, Department of Veterinary, Islamic Azad University, Yasooj, Iran
author
Ghazal
Raoofat
Master of Medicinal Plant Physiology, Expert of Agriculture Jahad Fars Province, shiraz, Iran
author
text
article
2021
per
Recently, studies have been conducted to use plant compounds as natural preservatives in foods. So, the objective of this study was first to evaluate antimicrobial activity of ethanolic and methanolic extracts of Aloe vera, German chamomile and Mentha piperita L cultivated in Medicinal plants farm of agricultural research institute of zabol university, against spoilage and pathogenic microorganisms associated with pasteurized milk including Pseudomonas aeruginosa, Bacillus cereus, Micrococcus luteus, listeria monocytogenes and Escherichia coli and the next stage was to investigate the impact of ethanolic extracts of aloe vera and German chamomile on the shelf life of pasteurized milk. The results indicated that Aloe vera had the most antimicrobial activity followed by German chamomile and Mentha piperita L respectively (P<0.05). In general, the ethanolic extract of studied plants was found to posses more powerful antibacterial activity than methanolic one (P<0.05). Ethanolic extracts of Aloe vera and German chamomile were evaluated as natural preservatives at concentrations of 0.15, 0.3 and 0.6 (% v/v). The results revealed that the treatments of pasteurized milk with 0.3% and 0.15% of German chamomile and also Aloe vera with a concentration of 0.3% with acceptable sensory properties had a significantly lower total microbial count and longer shelf life compared to the control sample. Therefore, this study confirmed the possibility of using of the extract of mentioned plants as a preservative in pasteurized milk besides its beneficial properties of a functional food.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
56
67
https://nfvm.uoz.ac.ir/article_136482_85d16a24e276b4140e38ef8732e2423c.pdf
dx.doi.org/10.22034/nfvm.2021.136482
Serological survey of Neospora caninum in female Markhoz goats of Saqqez district , Kurdistan
Hamed
Moosawi
Veterinary graduate, Faculty of Veterinary Medicine, Islamic Azad University of Sanandaj, Sanandaj, Iran
author
Ghazaaleh
Adhami
Assistant Professor, Department of Veterinary Pathobiology, Islamic Azad University of Sanandaj, Sanandaj, Iran
author
text
article
2021
per
Neospora caninum is a livestock protozoan parasite that causes reproductive damages in sheep and goats. Neospora caninum infection is a long-term complication and the host will be lifelong infected, and abortions caused by Neospora caninum may occur after any pregnancy after infection. The present study aimed to investigate the seroprevalence of Neospora caninum in female Markhoz goats of Saqqez County. To this end, we tested 180 serum samples by ELISA. Among them, 4 serum samples (2.2%) were positive. One (1.44%) out of 69 Markhoz goats under 1 years old and 3 (2.7%) out of 111 Markhoz goats over 1 years old were positive in terms of infection with Neospora caninum. The statistical examination indicated no significant relationship between the rate of infection and age groups (p>0.05). The statistical analyses also indicated a significant relationship between the rate of infection in sheep and history of abortion (p<0.05). The research results found the low environmental exposure of native goats to Neospora caninum. Despite the low prevalence of Neospora caninum in the present study, great attention should be paid to control strategies to avoid the risk of infection. Furthermore, more studies should be conducted to better evaluate its prevalence in these animals.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
68
76
https://nfvm.uoz.ac.ir/article_137029_2be04a2d09f8fd0153b503554eeed423.pdf
dx.doi.org/10.22034/nfvm.2021.137029
Characterzation of erythromycin resistance phenotypes and genotypes among vancomycin sensitive and vancomycin resistant Staphylococcus aureus isolated from bovine raw milk
hamid
adim
amol university
author
Razieh
Partovi
Assistant professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Mod-ern Technologies, Amol, Iran
author
Hamidreza
kazemeini
Assistant professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Mod-ern Technologies, Amol, Iran
author
Rahem
khoshbakht
Assistant professor, Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
author
Maryam
Azizkhani
Assistant professor, Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
author
text
article
2021
per
Staphylococcus aureus is the causing agent of endocarditis, sepsis and Staphylococcal food poisoning. The purpose of this study was to assess erythromycin sensitivity in 50 Vancomycin Resistant S. aureus (VRSA) and Vancomycin Sensitive S. aureus (VSSA) isolates from bovine raw milk and also to determine the distribution of erythromycin resistance genes among the isolates. Broth microdilution method was used to determine vancomycin resistance and minimum inhibitory concentration of erythromycin. The presence of the ermA, ermB, ermC and msrA genes encoding erythromycin resistance was examined by PCR. 84% of the isolates were susceptible while 16% were resistant to vancomycin. A single isolate of S. aureus was sensitive to erythromycin which did not possess any of the erythromycin resistance genes. 90% of the isolates were resistant to erythromycin, of which, no resistance gene were found in 24.4% and 51.1% were ermB positive. ErmA was not found in any of the isolates. Simultaneous presence of resistance genes was detected in eight isolates. There was not any significant relationship between erythromycin resistance genes and MICE. 37.5% of VRSA isolates contained none of the erythromycin resistance genes while 25% contained two resistance genes. Mean MICE of VRSA was higher than VSSA isolates. All the VRSA isolates were also erythromycin resistant. There was no significant relationship between erythromycin resistance genes and vancomycin sensitivity.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
77
88
https://nfvm.uoz.ac.ir/article_137561_cac7bd1d7a85a9bea09dd9faa81fc56c.pdf
dx.doi.org/10.22034/nfvm.2021.137561
Detection of antibodies against H5 subtype of Avian influenza in the egg yolks of native ducks in Gilan province, Iran, 2020.
Mohammad Hossein
Fallah Mehrabadi
Department of Poultry Diseases, RAZI Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
author
Hamideh
Najafi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Pouria
Motamed Chaboki
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Hossein
Hosseini
Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran
author
Abbas
Barin
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Zahra
ZiafatiKafi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Ali
Hojabr Rajeoni
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Iradj
Ashafi Tamai
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Naser
Sadri
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Arash
Ghalyanchi Langeroudi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2021
per
Avian influenza disease is one of the most significant contagious diseases that can infect many birds, either in commercial farms or in indigenous birds. There have been many reports on the highly pathogenic H5 subtype of avian influenza outbreaks from Iran's industrial poultry farms in recent years. Anseriformes, as the main reservoir of the virus, play a critical role in the epidemiology of the disease. This study was conducted to detect antibodies against the H5 subtype of avian influenza viruses in the yolk of native ducks. Seventy duck eggs were collected from different cities of Gilan province (Rasht, Anzali, SoomeSara, Fuman, Astaneh Ashrafie, Langroud und Chaboksar) from April to September of 2020. The aquatic phase of eggs was separate and subjected to the OIE approved HI standard test using H5 specific antigen. 11.43% of tested samples showed antibodies against the H5 subtype of influenza virus. This study demonstrates the circulation of the H5 subtype viruses among the native duck population of the north of Iran.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
89
96
https://nfvm.uoz.ac.ir/article_138089_120c65ca26369d0815dc503abdc1f709.pdf
dx.doi.org/10.22034/nfvm.2021.138089
Elimination of bacteremia caused by Mycoplasma haemofelis following dual therapy with doxycycline and ciprofloxacin
Reza
Azargoun
Assistant Professor, Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
vahid
mohamadi
Assistant Professor, Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
mahmood
mahmoodi
Master of Science, Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia Uni-versity, Urmia, Iran
author
shahin
ehteshamfar
Master of Science, Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia Uni-versity, Urmia, Iran
author
text
article
2021
per
Mycoplasma haemofelis is small wall-less bacteria with worldwide prevalence that infect erythrocytes and without effective treatment may cause death. The failure of most antibiotic therapy protocols to effective elimination of this potentially zoonotic agent highlights the necessity to determine an optimal treatment. A two-year-old domestic shorthair cat was referred to veterinary hospital of Urmia University with symptoms of lethargy, fever, jaundice and anemia. Clinical findings, the results of Giemsa stained blood smear and polymerase chain reaction on 16S rRNA confirmed the infection caused by M. haemofelis. Doxycycline (10 mg/kg PO q24h) was administered for 4 weeks. However, no clinical improvement was achieved and persistence of M. haemofelis infection was observed on cytology. After starting dual therapy with the addition of ciprofloxacin (20 mg/kg PO q24h) for two weeks, elimination of bacteremia was confirmed by cytological re-examination and negative result of polymerase chain reaction as the preferred diagnostic method. According to the authors' knowledge, this is the first report of bacteremia elimination caused by M. haemofelis using dual treatment with doxycycline and ciprofloxacin. It is suggested that this protocol be tried for more feline patients.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
97
103
https://nfvm.uoz.ac.ir/article_138263_1ea5d7f6a9756d406cb1a91e406b0ffa.pdf
dx.doi.org/10.22034/nfvm.2021.138263
The comparison of Antibiotic Resistance Patterns of Salmonella typhi-murium isolates and Escherchia coli separated from intestinal contents and the skin close to the perineum in dogs in Esfahan province.
Fahimeh
Negin tagi
Faculty of Veterinary Medicine, Islamic Azad University of Shahr Kord, Shahr Kord, Iran
author
saam
torkan
Assistant professor, Department of clinical science, Faculty of Veterinary Medicine, Islamic Azad University of Shahr Kord, Shahr Kord, Iran
author
text
article
2021
per
Salmonella and Escherichia coli are important bacteria that cause intestinal infections and diarrhea. The increment of salmonella and E.coli antibiotic resistance is considered as a major global problem. Since indoor dogs are known as potential reservoirs for these two bacteria, this study was designed for investigation of antibiotic resistance pattern of salmonella typhimurium and Escherichia coli isolated from rectal and perineal region skin swabs of these dogs in Esfahan province. Skin and rectal swabs obtained from 100 dogs referred to veterinary clinics, were cultured for bacterial isolation. After bacterial culture and isolation, salmonella and E.coli positive colonies were confirmed using Polymerase chain reaction. Then the presence of the most prevalent antibiotic resistance genes were investigated using PCR method and phenotypic patterns were determined using disc diffusion method. All of the E.coli bacteria isolated from rectal samples were resistant to gentamycin, tetracycline, ciprofloxacin, and trimethoprim- sulfamethoxazole. Furthermore, all of salmonella rectal isolates were resistant to tetracycline. dfrA1 gene was detected in all of E.coli bacteria isolated from rectal swabs. Tet B and dfrA1 were the most detected genes in salmonella typhimurium bacteria isolated from rectal swabs. Multiple antibiotic resistance in these isolates shows extra usage of antibiotics and to overcome this problem, antibiogram test is suggested to choose the best antibiotic for the treatment of infectious disease.
New Findings in Veterinary Microbiology
University of Zabol
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3
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2
no.
2021
104
117
https://nfvm.uoz.ac.ir/article_138878_ff2a433b32dec5097eab922b0f17f700.pdf
dx.doi.org/10.22034/nfvm.2021.138878
Molecular detection of Infectious Bronchitis virus in broiler chicken flocks
Najmeh
Motamed
Assistant Professor, Department of poultry vaccines research and production, Razi Vaccine and Serum Research Institute, Agricultural research, Education and Extension organization, Karaj,Iran
author
Mohsen
Beshashati
Assistant Professor, Department of Avian diseases research and diognostic, Razi Vaccine and Serum Research Institute, Agricultural research, Education and Extension organization, Ka-raj,Iran
author
mohammad hossein
Fallah
Assistant Professor, Department of Avian diseases research and diognostic, Razi Vaccine and Serum Research Institute, Agricultural research, Education and Extension organization, Ka-raj,Iran
author
text
article
2021
per
Infectious bronchitis disease is one the most important diseases in poultry industry worldwide. Although vast vaccination with different vaccines the virus causes major economic losses in commercial poultry production. The main object of this study was to detect infectious bronchitis virus infection in respiratory complex in broiler chicken flocks. Tissue samples from 35 flocks in central and south regions of Iran in 2018 and 2019 were collected and investigated by RT-PCR reaction. For phylogenetic analysis, Nucleotide sequence of partial S1 gene was amplified then evaluated. Totally from 35 flock with vaccination history against IBV and suffering from respiratory problems15 flocks were positive for IBV infection (42/85 percent). Two genotype was detected including GI-23 Is/1494 like (variant2) and Mass type ones. IS/1494 like viruses isolated in this study were clustered with other Variant2 viruses reported from Iran which has increasing trend recent years. Massachussete viruses were not clustered with Iranian mass type viruses including vaccinal strains and had some differences. Thereafter result of this study indicates that even with frequent vaccination, IBVs can cause outbreaks and severe problems in vaccinated chicken flocks.
New Findings in Veterinary Microbiology
University of Zabol
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3
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2
no.
2021
118
126
https://nfvm.uoz.ac.ir/article_139269_c59b00a92f24ee495792e7305ffb3135.pdf
dx.doi.org/10.22034/nfvm.2021.139269
Molecular Detection of Newcastle, Infectious Bronchitis, and Influenza (H9) viruses in Respiratory Complex Samples in Broiler Flocks of Goles-tan Province, 2019-2020
Mohammad
Montazeri
DVSc Student, Department of Avian Health and Diseases, Faculty of veterinary Medicine, University of Tehran, Teh-ran, Iran
author
Arash
Ghalyanchi Langeroudi
Professor, Department of microbiology and immunology, Faculty of veterinary Medicine, University of Tehran, Teh-ran, Iran
author
Naser
Sadri
Ph.D. Student, Department of microbiology and immunology, Faculty of veterinary Medicine, University of Tehran, Tehran, Iran
author
Zahra
ZiafatiKafi
Ph.D. Student, Department of microbiology and immunology, Faculty of veterinary Medicine, University of Tehran, Tehran, Iran
author
Mehdi
Vasfi Marandi
Professor, Department of Avian Health and Diseases, Faculty of veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2021
per
The expansion of the poultry industry and the intensive breeding of birds indoors have led to respiratory infections of several different infectious agents, especially avian influenza (AI), Newcastle disease (ND) and infectious bronchitis. Evidence suggest that these agents act synergistically with each other in affected flocks. Twenty broiler flocks with respiratory infection in Golestan province were selected. Trachea and kidney samples were transferred to the laboratory, and PCR was carried out to detect ND, IB, and H9 influenza viruses. According to the results, Flocks were more involved with IB infection (20%), and then ND (15%) and H9 influenza (10%) were more prevalent, respectively. Also, among these respiratory complex infections, the coinfection of IB with ND (20%) and AI (20%) viruses was the more common. According to the conclusions of this study, infectious bronchitis virus played the most crucial role in the development of respiratory complexes, and co-infection with Newcastle disease virus and Influenza H9 also causes severe symptoms and mortality in the Flocks. It is recommended that accurate epidemiological studies be conducted each year to determine the prevalence of risk factors of this disease and vaccines' efficiency in Flocks. This is the first comprehensive study on the respiratory complex of broiler herds in Golestan province, one of the centers of chicken meat production in the country.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
127
136
https://nfvm.uoz.ac.ir/article_140600_97519b177cad573fcbbad496859405af.pdf
dx.doi.org/10.22034/nfvm.2021.140600
The Inhibitory Effect of Nanocomplex of Ganoderma and Silver Fungus Extract on Biofilms of Bacteria that Cause Nosocomial Infections
Samira
Kadoughani Sani
Master of Microbiology, Department of Biotechcology, Sabzevar Branch, Islamic AzadUniversity of Sabzevar, Sab-zevar, Iran
author
Hamidreza
Farzin
Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organi-zation (AREEO), Mashhad, iran
author
Majid
Jamshidian-Mojaver
Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organi-zation (AREEO), Mashhad, iran
author
Mohadeseh
Amiri
Master of Bacteriology, Faculty of Veterinary Medicine, Shahid Bahonar University ofKerman, Kerman, Iran
author
text
article
2021
per
In general, nosocomial infections are those infections that are limited or diffuse and are caused by pathogenic reactions related to the infectious agent or its toxins in the hospital, provided that at least 48 to 72 hours After admission, the patient should be hospitalized and at the time of admission, the person should not have obvious signs of infection or be in the latent period of the disease. The causes of nosocomial infections are discussed. The bacteria used in this study included standard strains of Staphylococcus aureus (ATCC 14028), Acinetobacter baumannii (ATCC 6538) and Streptococcus pyogenes (19606 ATCC) and Pseudomonas aeruginosa (ATCC 27853). The bacteria used in this study were transferred to their specific media including McConkey agar (Pseudomonas aeruginosa), Parker agar board (Staphylococcus aureus), blood agar (Streptococcus pyogenes) and chocolate agar (Acinetobacter baumannii). Scanning electron microscope was used to measure the dimensions and shape of silver nanoparticles. Infrared spectroscopy was also performed to investigate possible organic compounds that could be involved in the synthesis of nanoparticles. Microplate method was used to investigate the effect of fungal and silver nanocomplexes in inhibiting the biofilm formation of bacteria used in this study. The results showed that there was a significant relationship between biofilm formation in the presence of nano-complex of Ganoderma and silver extracts; Among them, Staphylococcus aureus had a weak biofilm, Acinetobacter baumannii had no biofilm, Pseudomonas aeruginosa had a weak biofilm and Streptococcus pyogenes had a strong biofilm. Fungal and silver nanocomplexes have antibacterial activity in various concentrations and also this complex has a growth inhibitory concentration and also has the ability to inhibit biofilm formation. Due to the good antibacterial activity of this compound, this compound can be used in various medical applications.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
3
v.
2
no.
2021
137
144
https://nfvm.uoz.ac.ir/article_141929_e4245c3771795959b86964b7830f11ec.pdf
dx.doi.org/10.22034/nfvm.2021.141929