اثرات عصاره‌ی گل راعی و آلوئه‌ورا بر التیام زخم باز تلقیح شده با باکتری استافیلوکوکوس اورئوس در رت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

2 استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

3 دانشیار گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

4 استادیار گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

چکیده

باکتری استافیلوکوکوس اورئوس جزو فلور طبیعی پوست محسوب شده و عامل مهمی در عفونت زخم‌ها در انسان و حیوانات است. دو گیاه گل راعی و آلوئه‌ورا دارای اثرات ضد التهابی، آنتی‌اکسیدانی و ضد میکروبی قوی هستند و می‌توانند بر روی التیام زخم تأثیرات مثبتی بگذارند. در این مطالعه تعداد 40 رت به‌صورت تصادفی به 4 گروه تقسیم شدند: گروه شاهد،گروه کنترل مثبت و دو گروه درمانی پماد حاوی دو گیاه یعنی عصاره روغنی گل راعی و عصاره الکلی آلوئه‌ورا که با دو درصد متفاوت 1 و 4 درصد ، تحت درمان قرار گرفتند . نتایج بالینی و هیستولوژی با استفاده از آزمون‌های آماری آنوا و کروسکال-والیس با یکدیگر مقایسه شدند. تفاوت آماری معنی‌داری بین درصد التیام زخم در روز 3 در دو گروه درمان شده با پماد حاوی عصاره گل راعی و آلوئه‌ورا 1 و 4 درصد وجود دارد. در روز 14 بافت پوششی دو گروه درمان بهتر از دو گروه دیگر است. در روز چهاردهم در هر دو گروه پماد حاوی عصاره گل راعی و آلوئه‌ورا 1 و 4 درصد فولیکول‌های مو تشکیل شده بود اما غدد سباسه فقط در گروه عصاره 4 درصد مشاهده شد و کیفیت تشکیل بافت پوششی نیز در گروه عصاره 4 درصد بهتر از سه گروه دیگر بود. نتایج به‌دست آمده نشان می‌دهد که پماد حاوی عصاره روغنی گل راعی و عصاره الکلی آلوئه‌ورا 4 درصد در زخم تلقیح شده با باکتری استافیلوکوکوس اورئوس دارای اثرات التیام بخشی بهتری از نظر هیستوپاتولوژی و زیبایی است.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Hypericum perforatum and Aloe vera extracts in rat open wounds inoculated with Staphylococcus aureus: clinical and histopathol-ogy aspects

نویسندگان [English]

  • Shadi Sanadgol 1
  • Mohammad Reza Aghchelou 2
  • Darush Saadati 3
  • Abbas Jamshidian 4
1 Gradated student, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
2 Department of Clinical Science, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
3 Department of Food Hygiene, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
4 Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
چکیده [English]

Staphylococcus aureus is an important cause of wound infection and found on the skin of normal human and animal population. Hypericum perforatum and Aloe vera have antioxidant, anti-inflammatery and antibacterial effects, so they can affect on wounds positively together. For this purpose, 40 rats were selected and randomly divided into 4 groups: negative and positive control groups and two groups that treated with Hypericum perforatum and Aloe vera ointments that containing 1 and 4 percent extracts. The wounds were pictured on 0, 3,7 and 14 days after wound creation and the wound healing rates was calculated clinically. Histopathologic stadies were performed on samples collecteal from the wounds in 3,7 and 14 days after wound creation. Finally, the data were analyzed by Anova, Kruskal wallis test. The percentage of wound healing in 3th day in two groups treated with ointment containing extract was higher than other groups. on the 14th day the clinically epithelial tissue of the two groups treated with plant extracts was better than the other two groups. Appendices of the skin like hair follicles are formed in two groups contains extracts, but in the extract of %4 was observed more better than the other three groups becase the sebaceous glands were just observed in this group and quality of epithelial tissue was more better. The results show that ointment containing %4 Aloe vera and Hypericum perforatum extracts have better healing effects and skin beauty results than %1 ointment on wounds that inoculated with Staphylococcus aureus.

کلیدواژه‌ها [English]

  • words: Hypericum perforatum
  • Aloe vera
  • histopatology
  • wound healing
  • Staphylococcus aure-us

مراجع

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تازه ها در میکروب شناسی دامپزشکی
دوره 4، شماره 1
شهریور 1400
صفحه 1-22

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سابقه مقاله

  • تاریخ دریافت: 09 آبان 1400
  • تاریخ پذیرش: 09 آبان 1400

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