New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Carcasses of slaughtered goats in Kerman: A potential reservoir of β-lactam-resistant Escherichia coli and a public health challenge

Document Type : Original Article

Authors
Shirin Mohammadipour 1
Reza Ghanbarpour 2
Maziar Jajarmi 3
Mahboube Bagheri 4
1 Ph.D. Student, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
2 Professor, Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran
3 Assistant professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
4 Assistant Professor, Department of Food Science and Technology, Bardsir Faculty of Agriculture, Shahid Bahonar Uni-versity of Kerman, Kerman, Iran
10.22034/nfvm.2024.457540.1237
Abstract
Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is one of the significant food-borne pathogens transmissible from food products of animal origin to humans. The aim of this study was to investigate the phenotypic and genotypic resistance of E. coli isolates obtained from goat carcasses in Kerman abattoir against β-lactam antibiotics. In this study, 150 goat carcasses were examined at the Kerman slaughterhouse over a one-year period in 2023. From each carcass, two swab samples were collected, one from the internal surface and one from the external surface, resulting in a total of 300 swabs (150 carcasses × 2 swabs = 300 swabs). After culturing the swabs and isolating E. coli, the resistance of the isolates to cefotaxime and ceftazidime was determined using the disk diffusion method, and the genes blaTEM, blaSHV, blaCTX-M, and blaOXA were detected using PCR. Overall, 190 of 300 the swabs (63.33%) were positive for E. coli, resulting in 190 isolates. Among these, 45.27% (86 of 190 isolates) were resistant to cefotaxime, and 26.32% (50 of 190 isolates) were resistant to ceftazidime. The frequencies of the blaTEM, blaCTX-M, blaSHV, and blaOXA genes were 8.94% (17 of 190 isolates), 7.89% (15 of 190 isolates), 2.1% (4 of 190 isolates), and 2.1% (4 of 190 isolates), respectively. As a result, this study identifies goat carcasses as a potential reservoir of β-lactam-resistant E. coli, posing a significant public health challenge in the studied region.
Keywords
β-lactam
antibiotic resistance
Escherichia coli
goat carcasses

Subjects

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New Findings in Veterinary Microbiology
Volume 8, Spring
Spring 2025
Pages 11-21

  • Receive Date 14 May 2024
  • Revise Date 06 September 2024
  • Accept Date 07 September 2024