New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Assessing Furazolidone Antibiotic Residue in commercial eggs by ELISA method

Document Type : Original Article

Authors
Hadiseh Sanakhan Rezaiyeh
leila modiri
Arash Chaichi Nosrati
Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran
10.22034/nfvm.2024.474638.1252
Abstract
Furazolidone is an antibiotic from the nitrofuran family that has antimicrobial properties against gram-positive and gram-negative bacteria and is commonly used to treat bacterial infections such as enteritis and gastroenteritis. Additionally, this drug is used as a feed additive and growth promoter in the poultry industry. However, due to its toxic side effects and the risk of antibiotic resistance, the use of nitrofurans in poultry farming is prohibited in Iran. Therefore, this study investigates the residual metabolite of furazolidone (AOZ) in branded eggs purchased in Gilan province. In the present study, 646 branded eggs were randomly purchased and collected in three stages from different stores in Gilan province. Samples were tested utilizing RIDASCREEN® Nitrofuran (AOZ) Art. No. R3703 kit to examine the antibiotic furazolidone (AOZ) metabolite. The results were analyzed using SPSS software and one-way analysis of variance (ANOVA). Among the 646 egg samples collected, 412 samples (63.8%) were infected with furazolidone antibiotic. The mean concentration of furazolidone antibiotic residue was 119.04 ng/kg, and the lowest and highest values detected were 39.06 ng/kg and 749.1 ng/kg, respectively. The results indicate the presence of furazolidone antibiotic residues in egg samples. Therefore, it seems necessary to monitor the poultry breeding industry regularly due to the dangers of antibiotic residues in eggs and the prohibition of applying furazolidone.
Keywords
Antibiotic residue
furazolidone
AOZ
ELISA
egg

Subjects

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New Findings in Veterinary Microbiology
Volume 8, Spring
Spring 2025
Pages 46-55

  • Receive Date 21 August 2024
  • Revise Date 05 November 2024
  • Accept Date 11 November 2024