New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Prevalence of three NDM-1, oqxA and oqxB genes in Klebsilla pneumoni-ae isolates isolated from urine samples

Document Type : Original Article

Authors
Raziyeh Zahedi Nasab 1
Seyedeh Parisa Hasanein 2
Ahmad Rashki 3
1 MSC student, Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
2 professor, Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
3 Associate professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
10.22034/nfvm.2022.366612.1157
Abstract
Beta-lactamases and secretory pumps are the most important factors causing antibiotic resistance in Klebsiella pneumoniae bacteria due to the high prevalence of genes causing resistance and the spread of hospital infections, especially urinary tract infections caused by them, in In this study, Klebsiella pneumoniae isolates, isolated from urine samples were examined for the frequency of NDM-1, oqxA and oqxB genes using PCR technique.  DNA extraction was extracted by boiling method. PCR was done on 95 isolates of Klebsiella pneumoniae using the primers designed in this study. The showed that the prevalence of genes encoding antibiotic resistance including oqxA, oqxB and NDM-1 genes in 75 isolates (% 78.94), 75 isolates (78.94%) and 84 isolates (88.42%) respectively were evaluated. Prevalence of antibiotic resistance genes in this study is worrying and management of antibiotic prescription is necessary to control infection and prevent the spread of resistant bacteria and identify drug resistant isolates using molecular methods such as PCR.
Keywords
Klebsiella pneumoniae
antibiotic resistance
urinary tract infection
NDM-1
oqxA
oqxB

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  • Receive Date 21 October 2022
  • Revise Date 14 November 2022
  • Accept Date 21 November 2022