New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

The Antifungal Effect of Salix egyptica Extract on Malassezia pachydermatis (ATCC 10231) and its Comparison with Clotrimazole

Document Type : Original Article

Authors
Arian Nematinezhad 1
Issa Gholampour azizi 2
1 Graduate student, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran
2 Department of Mycology, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran
10.22034/nfvm.2022.361733.1155
Abstract
Malassezia pachydermatis, as an opportunistic yeast, exists on the skin of humans and animals and takes effect if the immune system is weak. Salix egyptica flower has antioxidant and cholesterol-lowering properties, and it is also used to relieve pain and inflammation. In the study, the inhibitory effects of aqueous, ethanolic, and methanolic extracts of this plant on this yeast were investigated. Aqueous, ethanolic, and methanolic extracts were prepared with a Soxhlet extractor. The disk and well diffusion, the determination of minimum inhibitory concentration (MIC), and the determination of minimum fungal concentration (MFC) were studied in the laboratory to evaluate the antifungal effects of these extracts on Malassezia pachydermatis. This yeast prepared of the collection microbial center shahriar with code ATCC 10231. The mean minimum inhibitory concentrations (MIC) on Malassezia packydermatis were 8.3 ×103 μg/ml for ethanolic extract, 1.2×104 μg/ml for methanolic, and 1.4 ×104 μg/ml for aqueous. MFC 20.8 ×104 μg/ml was in ethanolic extract, 1.6 ×104 μg/ml in methanolic extract, 5×104 μg/ml in aqueous extract. Findings indicate that alcoholic and aqueous extracts of Pussy willow have anti- Malassezia Pachydermatis effects, however, lower than Clotrimazole.
Keywords
Clotrimazole
Disc
Malassezia Pachydermatis
Salix egyptica flower
Well
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New Findings in Veterinary Microbiology
Volume 5, Issue 2 - Serial Number 10
October 2022
Pages 88-100

  • Receive Date 11 September 2022
  • Revise Date 29 October 2022
  • Accept Date 05 November 2022