New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Drug resistance and frequency of beta-lactamases genes of blaSHV and blaOXA of Escherichia coli isolated from broiler chickens

Document Type : Original Article

Authors
Hossein Noori 1
Mohammad Jahantigh 2
1 Graduated student, faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
2 Professor, Department of Clinical Sciences, faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
10.22034/nfvm.2023.393796.1185
Abstract
Escherichia coli is one of the most common pathogens in poultry that causes Colibasilosis. Until now many Extended-spectrum beta-lactamases (ESBLs) were identified that we can refer to SHV and OXA that they are coded by blaSHV and blaOXA, respectively. Regarding the development of antibiotics resistance and harmful effects of resistant bacteria on beta-lactam antibiotics, they can have an effect on human health and animal health, and due to the high mortality rate of disease, reducing production, as well as damage caused by drug use, it’s very important to control and prevention this disease. In the present study, 50 isolates of Escherichia coli were collected from 60 cloacal swabs from broiler chicken farms between 7 to 21 days old in Zabol. Antibiogram test was performed by disk diffusion method to investigate drug resistance to Ceftriaxon, Cefazolin, Ceftizoxime, Cephalothin, Ceftazidime and Cefixime. Besides, the frequency of blaSHV and blaOXA were investigated by PCR. The results showed that the highest resistance was to Cefazolin (80%) and the lowest resistance was to Ceftriaxon (8%). The frequency of blaSHV and blaOXA genes in Escherichia coli isolates was 24% and 0.0%, respectively.
Keywords
Escherichia coli
Drug resistance
ESBLs
blaSHV
blaOXA

Subjects

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New Findings in Veterinary Microbiology
Volume 7, Issue 1
May 2024
Pages 1-9

  • Receive Date 26 April 2023
  • Revise Date 10 June 2023
  • Accept Date 11 September 2023