New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Prevalence of genes encoding exotoxins in methicillin-resistant and sensitive Staphylococcus aureus isolated from mosquito larvae in Sistan region by PCR method

Document Type : Original Article

Authors
Seyedeh Parisa Hasanein 1
Vahideh Hamidnia 2
1 Department of Biology, faculty of Basic Sciences, University of Zabol, Zabol, Iran
2 Master's student, Department of Pathobiology, faculty of Veterinary, University of Zabol, Zabol, Iran
10.22034/nfvm.2024.461253.1247
Abstract
Staphylococcus aureus produces a wide range of exotoxins that makes diseases in the host. Pyrogenic superantigens are a group of exotoxins produced by Staphylococcus aureus. This group includes toxic shock syndrome toxin, exfoliative and all enterotoxins of this bacterium. Identifying these factors is essential in understanding the pathogenic process of bacteria. The aim of this study was to investigate the presence of toxic shock syndrome (TSST-1), exfoliative A/B and enterotoxins A, B, C, D, E genes in methicillin-resistant and sensitive Staphylococcus aureus isolated from mosquito larvae in Sistan region by PCR method. This research was conducted on 57 isolates of Staphylococcus bacteria. Methicillin-resistant and sensitive isolates were identified using agar diffusion method and oxacillin-containing DNA plates. Bacteria were extracted by boiling method. Using PCR method and specific primers, genes encoding toxic shock syndrome (tst), exfoliative (eta, etb) and enterotoxin (sea, seb, sec, sed, see) were investigated in this bacterium. Out of a total of 57 Staphylococcus aureus isolates, 32 resistant isolates and 25 methicillin sensitive isolates were identified. The frequency of enterotoxin, exfoliative and toxic shock syndrome genes in resistant isolates was 50%, 21.87%, 3.12% respectively and in sensitive isolates 44,08%, 23,75% and 0% respectively. The results showed that the prevalence of methicillin-resistant isolates and isolates capable of carrying enterotoxin and exfoliative genes was high in Sistan region.
Keywords
antibiotic resistance
Staphylococcus aureus
mosquito larvae
methicillin
entrotoxin

Subjects

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New Findings in Veterinary Microbiology
Volume 7, Issue 2
December 2024
Pages 1-9

  • Receive Date 05 June 2024
  • Accept Date 29 August 2024