New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

The effect of adding different levels of selenium-yeast supplement and selenium nanoparticles on the population of protozoa and rumen ammonia concentration of Baluchi fattening male lambs (native of Sistan)

Document Type : Original Article

Authors
Hossein Ansari nik 1
Mohammad Reza Dehghani 2
Mostafa Yousef-Elahi 2
Amir Mousaie 3
Ahmad Rashki 2
Arash Omidi 4
1 Ph.D. student, Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran
3 Assistant Professor, Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
4 Professor, Department of Animal Health Management, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
10.22034/nfvm.2024.460687.1245
Abstract
This study was conducted to investigate the effect of dietary supplementation of selenium nanoparticles (SeNP) and selenium yeast (SY) on ruminal protozoan population and ammonia nitrogen of Baluchi male lambs. Thirty Baluchi lambs with an average initial body weight of 21.56±0.33 kg were used in a completely randomized design with 5 treatments and 6 repetitions for 70 days (14 days of adaptation and 56 days of data collection). The experimental groups were 1) control (without selenium supplement), 2), 3) and 4) were feeding with 0.4, 0.8 and 1.2 mg Se/kg of diet as SY respectively, and 5) feeding with 0.4 mg Se/kg of diet as SeNP. The results showed that the number of protozoa in the ruminal fluid of lambs receiving 0.8 and 1.2 mg of Se/kg of diet as SY and 0.4 mg of Se/kg of diet as SeNP was higher than that of the control group (P<0.05). In addition, both SY and SeNP sources significantly increased the number of Ophryoscolex species, while 0.4 mg of Se as SeNP resulted in a significant increase in Dasytricha species (P<0.05). Furthermore, dietary supplementation of 0.8 mg of Se as SY and 0.4 mg of Se as SeNP increased the number of Diplodinium species (P<0.05). However, the populations of Entodinium and Isotricha species were not affected by the experimental treatments. Feeding with 0.8 and 1.2 mg of Se as SY/kg of diet and 0.4 mg of Se as SeNP/kg of diet led to decreased ruminal ammonia nitrogen quantity (P<0.05). The results of this research indicated that dietary selenium supplementation as Nano selenium and selenium yeast, can increase the protozoa population and decrease the concentration of ammonia nitrogen in the ruminal fluid of Baluchi fattening lambs.
Keywords
Fattening lamb
Protozoa count
Selenium
Yeast

Subjects

1- Al-Saadi H, Mousaie A, Esmaeilipour O A, Ziaei N. Effect of organic selenium supplement on growth performance, nutrient digestibility and some blood metabolites of Kermani fattening lambs. J Res Rum. 2017; 6(2): 17-30. [In Persian]
2- Barceloux DG. Selenium. J Clin Toxicol. 1999; 37: 145-172.
3- Broderick GA. Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci. 1980; 63(1): 64-75.
4- Dayrell MD, Ivan M, Hidiroglou M. The effects of ruminal protozoa on selenium status in sheep. Can.J.Anim.Sci. 1991; 71: 1269–1270.
5- Dehority B. Laboratory Manual for Classification and Morphology of Rumen Ciliate Protozoa; CRC Press: Boca Raton, FL, USA, 1993.
6- Dehghani S, Taghizadeh A, Mohammadzadeh H. The effect of different forms of selenium on parameters of gas production, rumen fermentation and rumen protozoa population. J anim sci res I. 2018; 11(3): 307-317 [In Persian]
7- Faixova Z, Faix S, Leng L, Vaczi P, Makova Z, Szaboova R. Hematological, blood and rumen chemistry changes in lambs following supplementation with Se-yeast. Acta Vet Bru. 2007; 76: 3-8.
8- Fujihara T, Imamura T, Orden EA. Utilization of protozoal selenium in young Goats. J Ani Feed Sci. 2004; 13: 265-268.
9- Getachew G, Makkar HPS, Becker K. Tropical browses: content of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acids and in vitro gas production. J Agri Sci. 2002. 139: 341-352.
10- Ghorbani A, Noorian Sarwar M A, Moini MM. The effect of zinc and selenium organic supplements on feed consumption, digestibility and parameters of ruminal fermentation in sheep. J Anim Scie. 2016; 30(115): 17-36. [In Persian]
11- Gruby D, Delafond HMO. Recherches sur des animalcules se développant en grand nombre dans l’estomac et dans les intestins, pendant la digestion des animaux herbivores et carnivores. Comptes Rendus Acad. Des Sci. 1843; 17: 1304–1308.
12- Hidiroglou M, Jenkins KJ. Influence of defaunation on the utilization of selenomethionine in the sheep. Ann Biol Anim Biochim Biophys. 1974; 14: 157–165.
13- Hungate RE. The Rumen and Its Microbes; Academic Press: New York, NY, USA, 1966.
14- Juszczuk-Kubiak E, Bujko K, Cymer M, Wicińska K, Gabryszuk M, Pierzchała M. Effect of inorganic dietary selenium supplementation on selenoprotein and lipid metabolism gene expression patterns in liver and loin muscle of growing lambs. Biol Trace Elem Res. 2016; 172: 336–345.
15- Mihalikova K, Gresakova L, Boldizarova K, Faix S, Leng L, Kisidayova S. The effects of organic selenium supplementation on the rumen ciliate population in sheep. Folia Microbiol. 2005; 50: 353-356.
16- Naziroglu M, Aksakal M, Cay S, Celik S. Effects of vitamin E and selenium on some rumen parameters in lambs. Acta Vet Hung. 1997; 45: 447–456.
17- Naziroglu M, Aksakal M, Cay M, Celik S. Effects of vitamin E and selenium on rumen protozoa in lambs. Tur J Vet Ani Sci. 1997; 21: 81–90.
18- NRC. Mineral Tolerance of Animals. National Academies Press, Washington, DC. USA. 2005; 493 pp.
19- Ogimoto K, Imai S. Atlas of Rumen Microbiology. Japan Scientific Society Press, Tokyo. 1981.
20- Pernthaler J. Predation on prokaryotes in the water column and its ecological implications. Nat Rev Microbiol. 2005; 3: 537-46.
21- Ramirez BJE, J L. TortoraHernandez, and M. Huerta. Main causes of mortalities in dairy goat kids from the Mexican plateau. S Rum Res. 2001; 41: 77-80.
22- Shi L, Xun W, Yue W, Zhang C, Ren Y, Liu Q, et al. Effect of elemental nano-selenium on feed digestibility, rumen fermentation, and purine derivatives in sheep. Ani Feed Sci Tec. 2011; 163: 136–142.
23- Underwood EJ, Suttle NF. The mineral nutrition of livestock. CAB international, Wallingford, U.K. 1999.
24- Wang C, Liu Q, Yang WZ, Dong Q, Yang XM, He DC, et al. Effects of selenium yeast on rumen fermentation, lactation performance and feed digestibilities in lactating dairy cows. Liv Sci. 2009; 126: 239-244.
25- Williams AG, Coleman GS. The Rumen Protozoa; Brock/Springer Series in Contemporary Bioscience; Springer: New York, NY, USA, 1992.
26- Wilsdorf VG, Heinze W, Krengel B. Studies into the action of sulfamerazine, sulfur, selenium, and/or vitamin E on rumen infusoria of cattle. Mh.Vet.Med. 1984; 39: 700–703.
27- Wright PL, Bell MC. Comparative metabolism of selenium and tellurium in sheep and   swine. Am J phys. 1966; 211: 6-10.
28- Xun W, Shi L, Yue W, Zhang CH, Ren Y, Liu Q. Effect of High-Dose Nano-selenium and Selenium-Yeast on Feed Digestibility, Rumen Fermentation, and Purine Derivatives in Sheep. Biol Trace Elem Res. 2012; 150: 130-136.
29- Alavi M, Rai M, Martinez F, Kahrizi D, Khan H, Rose Alencar de Menezes I, et al. The efficiency of metal, metal oxide, and metalloid nanoparticles against cancer cells and bacterial pathogens: different mechanisms of action. Cell Mol Biomed Rep. 2022; 2(1): 10-21.
New Findings in Veterinary Microbiology
Volume 7, Issue 2
December 2024
Pages 63-71

  • Receive Date 02 June 2024
  • Revise Date 12 June 2024
  • Accept Date 29 August 2024