New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Investigation of the antimicrobial properties of fennel and green cumin essential oils on strains of Enterobacter faecalis and Acinetobacter baumannii under laboratory conditions

Document Type : Original Article

Authors
Yahya Moradi 1
Mehdi Roshdi Maleki 2
1 Graduated student, Department of Microbology, Malekan Branch, Islamic Azad University, Malekan, Iran
2 Assistant Professor, Department of Microbology, Malekan Branch, Islamic Azad University, Malekan, Iran
10.22034/nfvm.2024.455730.1236
Abstract
To combat the shortage of new antimicrobial compounds and the increase in antibiotic resistance, plants could be a potential solution. The aim of the present study is to investigate the antimicrobial properties of fennel and green cumin essential oils against strains of Enterococcus faecalis and Acinetobacter baumannii under laboratory conditions. In this method, the essential oil solution was added at a specific concentration to sterile discs and transferred to a microbial culture medium. To examine growth inhibition, the disc diffusion method was used with a bacterial suspension on a plate containing Mueller-Hinton agar, and the MIC and MBC of the essential oil were also examined at dilutions of 101 and 1010, and finally, the optical density was measured at a wavelength of 540 nm. The results of the growth inhibition by the disc diffusion method showed that both bacteria, Acinetobacter baumannii and Enterococcus faecalis, created a 1.2 mm diameter. The results of MIC and MBC of green cumin essential oil on Acinetobacter baumannii at a dilution of 103 (MIC) showed an inhibitory effect at 0.078 in a concentration of 2000 ppm, and at MBC in a dilution of 102, it had a lethal effect at 0.135 in this bacterium. In Enterococcus faecalis, at a dilution of 101, both MIC and MBC had an inhibitory and lethal effect at 0.311 in a concentration of 2000 ppm. Given the spread of drug resistance in pathogenic strains, medicinal plants with antimicrobial effects are a suitable option with fewer side effects.
Keywords
Acinetobacter baumannii
Enterococcus faecalis
green cumin essential oil
fennel essential oil
drug resistance

Subjects

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New Findings in Veterinary Microbiology
Volume 7, Issue 2
December 2024
Pages 93-103

  • Receive Date 04 May 2024
  • Revise Date 13 June 2024
  • Accept Date 29 August 2024