New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Identification of phylogenetic groups of Escherichia coli isolated from colibacillosis in poultry by multiplex-PCR

Document Type : Original Article

Authors
Davood Tarbiat-Nazloo
Abolfazl Jafari-Sales
Yashar Bagherizadeh
Mahboubeh Abdoli-senejani
Farhad Farhadi
Mehdi Ezdiyadi
10.35066/J040.2018.483
Abstract
Among diseases caused by Escherichia coli, there is a severe systemic form termed colisepticaemia, which is characterized by the presence of E. coli in the blood, and colonization of organs including the heart, liver and spleen. The aim of the present study was to investigate different phylogenetic groups of E. coli isolated from broiler breeder with colibacillosis in Urmia. In this study, eighty swabs collected from liver and lung were cultured on MacConkey agar plates. Pink color colonies were isolated and confirmed as E.coli by biochemical tests and followed by multiplex-PCR to identify different phylogenetic groups. Out of 80 samples 21 isolates were identified as E.coli. Eight of isolates (38%) were belong to group A, 2 of them (9.5%) were belong to group B1, 6 of them (28.6%) were belong to group B2 and 5 of them (23.8%) were belong to group D2. According to the results of present study different phylogenetic group were observed in breeder herds. Most of them were classified as group A which is commensal. Studies showed that pathogenic E.coli has a considerable antibiotic resistance rate which might be transmitted to broilers in different ways and poses economic constraint to poultry industry. Thus, important strides must be made on eradication of different pathogenic E.coli.
Keywords
phylogenetic group
E.coli
poultry
Colibacillosis
  1. Aarestrup FM, Wegener HC, Collignon P. Resistance in bacteria of the food chain: epidemiology and control strategies. Expert Rev Anti Infect Ther. 2008; 6:733–750.
  2. Nolan, LK, Barnes, HJ, Vaillancourt, JP, Abdul-Aziz, T, Logue, CM. In: diseases of poultry.12thedition.   (Swayne, D.E., Mcdougald, L., Nolan, K., Suarez, D.L., Nair, V).  Iowa, USA: John Wiley and Sons. 2013; 751-805.
  3. Barnes HJ, vaillancourt JP, Gross WB. Colibacillosis in:  Diseases  of    Editedby Y. M. Saif, B. W. Calnek, H. J. Barnes, C. W. Beardand L. Macdougol. 11thed. Iowa University press, Iowa, USA, 2003:631-647.
  4. Wary C, Davies RH. In: poultry diseases. Edited by F.T. W. Jordan, M. Pattison, D. Alexander, and T. Foragher, 5thEd. W. b. Saunders Company, U.S.A., 2002; 125-130.
  5. Peighambari, SM, Vaillancourt, JP, Wilson, RA, Gyles CL. Characteristics of Escherichia coli isolates from avian cellulitis. Dis. 1995; 65:116-124.
  6. Skjøt-Rasmussen L, Olsen SS, Jakobsen L, Ejrnaes K, Scheutz F, Lundgren B, et al. Escherichia coli clonal group A causing bacteraemia of urinary tract origin. Clin Microbiol Infect. 2013; 19(7):656-61.
  7. Xia, X, Meng, J, Zhao, S, Bodeis-jones, S, Gaines, SA, Ayers, SL, et al. Identification and antimicrobial resistance of pathogenic Escherichia coli from retai meats. J Food Prot. 2011;74:38-44.
  8. Ghanbarpour R, Daneshdoost S. Identification of shiga toxin and intimin coding genes in Escherichia coli isolates from pigeons (Columba livia) in relation to phylotypes and antibiotic resistance patterns. Trop Anim Health Prod. 2012;44:307-12.
  9. Asadi S, Solhjoo, K, Kargar M, Rezaeian A. Phylogenetic groups of Escherichia coli strains isolated from urinary tract infection in Jahrom city, southern Iran. Journal of Microbial World. 2011; 3(4): 245-250. [In Persian]
  10. Etebarzadeh Z, Oshaghi M, Amir Mozafari N. Evaluation of Relationship between Phylogenetic Typing and Antibiotic Resistance of Uropathogenic Escherichia coli. J Microbiol World. 2012; 4(3&4):84-92.
  11. Alizade H,Ghanbarpour R, Aflatoonian MR, Abdollahi H. Determination of phylogenetic background, fimbrial genes, and antibiotic susceptibility of Escherichia coli isolates from urinary tract infections in Bam region, Iran. Comp Clin Path. 2014; 23(5):1253–1257.
  12. Abdi HA, Rashki A. The phylogenetic study of Uropathogenic Escherichia coli strains in Sistan of Iran. J Birjand Univ Med Sci. 2014; 21(3):385-393.
  13. Mikaili P, Ameghi A, Shayegh J, Hassani B, Mahmmudzadeh M. Phylogenic typing of Escherichia coli isolated from broilers with collibacillosis in Tabriz, North West of Iran. Arch Razi Inst. 2013; 68(1):43-46.
  14. Rodriguez-siek KE, Giddings CW, Doetkott C, Johnson TJ, Nolan LK. Characterizing the APEC. pathotype. Vet Res.2005;36:241-56.
  15. Kariyawasam S, Scaccianoce JA, Nolan LK. Common and specific genomic sequences of avian and human extraintestinal pathogenic Escherichia coli as determined by genomic subtractive hybridization. BMC Microbiol. 2007;7: 81.
  16. Obeng AS, Rickard H, Ndi O, Sexton M, Barton M. Antibiotic resistance, phylogenetic grouping and virulence potential of Escherichia coli isolated from the faeces of intensively farmed and free range poultry. Vet Microbiol. 2012;154:305-15.
  17. Sohrabi R, Zeighami H. Determination of Phylogenetic Groups and Antibiotic Resistance in Uropathogenic and Commensal Escherichia Coli Isolated from Patients in Zanjan City. Journal of Zanjan University of Medical Sciences & Health Services. 2016; 24(107):107-118.

 

New Findings in Veterinary Microbiology
Volume 1, Issue 2
April 2019
Pages 89-94

  • Receive Date 09 December 2017
  • Revise Date 25 December 2017
  • Accept Date 12 February 2018