Reconstitution of Gene Network on Penicillin Resistance in E.coli Using Databases Information
Yasoub
Shiri
faculty member
author
Mohammad ali
Karimiyan
Lecturer, Research Dept. Of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran.
author
text
article
2020
per
The discovery of penicillin has revolutionized the medical industry and saved thousands of lives. But over time, the resistant bacteria to this antibiotic were identified one after another. Antibiotics of the penicillin family have a β-lactam ring in their molecular structure. By competitively binding to transpeptidase, penicillin and other β-lactam antibiotics inhibit its activity. Inhibition of transpeptidase activity prevents cell wall synthesis and causes bacterial death. Resistant bacteria, have β-lactamase enzyme which has the ability to break the C-N bond in the β-lactam ring. In this study, we reconstructed a network of known β-lactamase genes in E.coli. The results showed that ampC gene plays a central role in induction of resistance in E.coli. Molecular function analysis of other β-lactamase genes showed that β-lactamase activity is only a secondary function for them and their main function is to participate in other unrelated cellular processes. Gene ontology analysis showed that the process of resistance to β-lactam antibiotics was positively correlated with electron transport chain. Also, when exposed to β-lactam antibiotics, the cell membrane is the most active cellular component in E.coli and antibiotic resistance is dependent on the β-lactamase function of cell membrane proteins.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
1
9
https://nfvm.uoz.ac.ir/article_105952_7bb3e38144055e0fae74b7e9db1f5d2f.pdf
dx.doi.org/10.35066/J040.2019.701
The study of florfenicol residue in chicken meat in Charmahal-va-Bakhtiyari province by high purified liquid chromatography
Mazyar
Mohammadi
Islamic Azad University, Shahrekord Branch
author
Ezzatollah
Fathi
Islamic Azad University
author
Majid
Gholami-Ahangaran
دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
author
text
article
2020
per
In spring 2016, 25 poultry meat samples were collected from slaughterhouses in Shahrekord and tested by HPLC for fluorophenyl residues in meat. The samples were extracted after preparation and mixed with hydrochloric acid and transferred to the cartridge. After purification in 20 µl volume, the samples were injected into HPLC. The results showed that out of 25 samples, 5 samples (20%) had florfenicol residues, one of which had a higher concentration of florfenicol residues in poultry meat. The minimum concentration of florfenicol in the studied samples was 15.6 µg / kg and the maximum concentration was 119.07 μg /Kg. In general, given the widespread use of antibiotics in poultry farming, it is better to have more control over the use of antibiotics, to reduce the antibiotic residues in poultry meat.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
10
16
https://nfvm.uoz.ac.ir/article_107094_f957f2722b92d4fdaf6cb7a8aad24d87.pdf
dx.doi.org/10.35066/J040.2019.913
The effect of aqueous extract of Urtica dioica leaf on hematological parameters in rats
Mehdi
Rasekh
Department of clinical sciences, faculty of veterinary medicine, university of Zabol, Zabol, Iran
author
kiandokht
Miri
Graduated student, faculty of veterinary medicine, university of Zabol, Zabol, Iran
author
text
article
2020
per
The use of medicinal plants has a special importance in medicine because of its therapeutic properties, low side effects, availability, and ease of use. Nettle is a medicinal plant found in most parts of Iran and has unique properties in traditional medicine such as the treatment of anemia. In this study, the effect of aqueous extract of Urtica dioica leaf on hematological parameters in rats was investigated. For this purpose, 16 rats were studied and divided into two groups of 8 cases. Each rat in the treatment group received 200 mg/kg of aqueous extract of nettle leaf orally for 28 days and blood sampling was performed on days 0 and 28 and hematological parameters were measured and finally, hematological parameters of control rats that did not receive nettle were compared and the obtained parameters were analyzed. The mean cell volume (MCV) was significantly increased in the treatment group (p=0.044), which, along with parameters such as hemoglobin, red blood cell count and hematocrit, showed an increase compared to the control group. The results of this study indicate that oral administration of aqueous extract of nettle leaf can have hematopoietic effects on rats.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
17
24
https://nfvm.uoz.ac.ir/article_106007_1da65ede6d559169ced26e759a50903f.pdf
dx.doi.org/10.35066/J040.2019.641
Serological identification of subclinical chicken anemia virus in quails, in Isfahan
Running title: Chicken anemia virus in quails
Majid
Gholami-Ahangaran
دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آراد اسلامی، شهرکرد، ایران.
author
Asiye
Ahmadi-Dastgerdi
Department of Food Science and Technology, Ardestan Branch, Islamic Azad University, Isfahan, Iran.
author
Mohammad Reza
Shahiri
Graduated of Veterinary Medicine Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
author
text
article
2020
per
Chicken anemia virus (CAV) is one of the immune-suppressive agents in the fowls. Although, fowls are the only natural host of this virus, there is some evidence that other avian species are susceptible to the virus or the virus can replicate in this bird. Therefore, considering the complications of inappropriate response to Newcastle disease vaccine, frequent bacterial contamination and inappropriate growth in quail farms; in this study, the epidemiological situation of CAV infection was investigated in quail farms by serological method. To this end, 150 serum samples from 15 quail farms in Isfahan were collected. After preparation, the CAV specific antibody was detected by one commercial CAV Elisa kit, and the Newcastle titer was tested by hemagglutination inhibition (HI) test. The results showed that 60% of the farms had at least one positive serum sample against the CAV that was evaluated from a total of 40% positive serum samples. In this study, although Newcastle vaccine was used in all farms, no prominent titer was detected at slaughter time. Therefore, it seems that CAV can also lead to immune suppression in quails in the subclinical form.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
25
31
https://nfvm.uoz.ac.ir/article_106068_a6506d90d18cae8e8baf2f8b534f5109.pdf
dx.doi.org/10.35066/J040.2019.149
Isolation and identification of Cryptosporidium spp. in diarrhetic calves in Alborz province
Gholamreza
Karimi
مؤسسه تحقیقات واکسن و سرمسازی رازی، کرج
author
habibolah
paikari
Razi vaccine and sera research institutes
author
Shahla
Rivaz
Razi vaccine and sera research institutes
author
Vahid
Nasiri
Razi vaccine and sera research institute
author
Mohammad
Abdi godarzi
Razi vaccine and sera research institute
author
Mohammad mehdi
Ranjbar
Razi vaccine and sera research institutes
author
text
article
2020
per
Crytosporidiosis is a parasitic disease caused by the cryptosporidium protozoan in the vertebrate animal. This parasite in livestock special ruminants causes diarrhea syndromes in infants. The main sources of the parasite spread are infectious feces and the host mouth. The purpose of this project was to isolate and identify the cryptosporidium species by morphological indices in diarrheic calves in order to detect and control the disease. According to the method of Casemore et al., purification of oocysts from feces using flotation technique was carried out. Staining with modified Zeil nelson method (Henrisksen method) and identification with diagnostic keys was done. Cryptosporidium parvum alone causes clinical disease in young calves; the latent infection time is 3 to 6 days. Cryptosporidium muris, C. Andersoni and C. ryanae have been reported in weaned calves, and C. Andersoni has been also observed in adult cattle. The total of 150 cattle fecal samples were randomly collected from Alborz province. 60 out of 150 (40%) calf diarrhea positive samples were detected by modified Zeil nelson method staining. In all fecal samples from calves without diarrhea, the oocyst was not observed. In calves below one month, especially in the first two weeks, 40% of the diarrheic samples were infected. This protozoon is a zoonotic and opportunistic parasite and, in Iran, it has been reported from various species like poultry, cattle, sheep and goats and it may cause significant economic losses which need much more attentions.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
32
40
https://nfvm.uoz.ac.ir/article_106243_520ba8971badd9c0f8ce8872805a4dbc.pdf
dx.doi.org/10.35066/J040.2019.261
Antimicrobial and anti-biofilm effects of Thyme essential oils and Peppermint on Acinetobacter baumannii and Staphylococcus aureus resistant to different antibiotics
fardin ali
malayeri
دانشکده پزشکی، گروه بیوشیمی، دانشگاه علوم پزشکی زابل، زابل، ایران
author
zahra
yazdanpour
کارشناسی ارشد میکروبیولوژی، دانشگاه علوم پزشکی زابل، زابل، ایران
author
hossein
bandani
دانشجوی دکتری علوم دام، دانشگاه ملی زابل، زابل، ایران
author
bahman
fazeli nasab
uoz
author
saeide
saeidi
university of Zabol
author
text
article
2020
per
The aim of this study was to investigate the antimicrobial and anti-biofilm effects of peppermint and Thyme on the antibiotic-resistant Acinetobacter baumannii and Staphylococcus aureus. Staphylococcus aureus and Acinetobacter baumannii strains were isolated from hospitals in Zabol city. The essential oil of Shirazi thyme and peppermint was obtained by Clevenger apparatus. Minimum lethal concentration and minimum inhibitory concentration were determined by microdilution method. Results showed that the lowest inhibitory concentration of peppermint essential oil against Acinetobacter baumannii and Staphylococcus aureus was 1.25 mg/ml. The results of our study on Thyme showed that the lowest concentration of Thyme essential oil against Acinetobacter baumannii and Staphylococcus aureus was 1.25 and 0.31 mg / ml, respectively. The results indicated that peppermint essential oil and Thymus vulgaris inhibited the growth of Staphylococcus aureus and Acinetobacter.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
41
51
https://nfvm.uoz.ac.ir/article_106288_f0a6cbb019aabd5d737bc17f01b9d55c.pdf
dx.doi.org/10.35066/J040.2019.697
Review on viral agents caused rinder pest like mucosal diseases in Iran
Roozbeh
Fallahi
Associate Professor, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
author
text
article
2020
per
Mucosal viral infections cause the most important and the most difficult diseases. Every year there are numerous reports of mucosal diseases that cause huge damage to the country's livestock industry. Bovine Viral Diarrhea-Mocusal Disease, Infectious Bovine Rhinotracheitis, and Malignant Catarrhal Fever are the most important viral mucosal diseases of the bovine species. A study of the reported foci of these diseases over the last two decades indicates that epidemiological units, especially industrial livestock, are circulating annually and have inflicted economic damage on these units. Unfortunately, there has been no comprehensive study program on the strains producing the mentioned diseases in the country and the studies have been limited to the evaluation of serum assessments. In this study, while introducing and describing the mentioned diseases in Iran and other countries, the problems and limitations for their control are discussed and after determining the strengths, weaknesses, opportunities and threats, the SWOT method and related strategies are analyzed and introduced to disease control.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
52
65
https://nfvm.uoz.ac.ir/article_106359_694ce11be505aa9a7fd8d8be874dbf3b.pdf
dx.doi.org/10.35066/J040.2019.411
Isolation and identification of Mycoplasmas which cause contagious Aga-lactia from sheep & goats in Tehran province
Seyed Jalal
Mirian
مؤسسه تحقیقات واکسن و سرمسازی رازی، کرج
author
text
article
2020
per
Agalactia, has been known as a contagious disease in Mediterranean countries, Africa, Europe, and west Asia e.g. Iran where the livestock were reared massively for approximately several years ago and it has been led to noticeable economic damages and losses. This disease appears by symptoms such as fever, inappetence, lameness and loss of milk in dairy ewes, reduction to total lack of lactation and abortion in pregnant livestock. No study has been so far conducted on isolation of Agalactia disease factor and determining its molecular identity in ovine and caprine herds in Tehran province. The current research aimed to isolate and determine molecular identity of Agalactia disease factor (Agalactiae mycoplasma) in sheep and goat that suffered from Agalactia, using culture and PCR methods in Tehran province. Among the total 33 taken samples, 14 samples were confirmed as mycoplasma species that showed specific band (bp163) on agarose gel and in one of these 14 given samples, the culture was positive.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
66
74
https://nfvm.uoz.ac.ir/article_107109_ca596d8d1a59632b8e5e2c06f444e2e6.pdf
dx.doi.org/10.35066/J040.2019.273
Molecular detection of Crimean Congo hemorrhagic fever in tick vectors in rural areas of eastern Iran
Amirsajad
Jafari
Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Mehdi
Rasekh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
author
Dariush
Saadati
گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران.
author
Faezeh
Faghihi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
author
Mehdi
Fazlalipour
Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran
author
Sahar
Khakifirouz
Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran
author
Tahmineh
Jalali
Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran
author
zahra
ahmadi
Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran
author
text
article
2020
per
Crimean Congo Hemorrhagic Fever (CCHF) is a deadly viral infection (mortality rate between 3 and 30%) reported from more than 30 countries. The disease is common between humans and animals and can be transmitted through tick bites, contact with blood and secretions or carcasses of infected animals and humans. The aim of this study was to determine the prevalence of CCHF virus in ticks isolated from domestic livestock in rural areas of Birjand City in South Khorasan Province. In this study, 390 livestock including 167 sheep, 205 goats, 9 cows and 9 camels were sampled in four villages (Nofarest, Hassanabad, Amirabad and Shaukatabad) of Birjand County. Eight species of hard ticks were identified, including Rhipicephalus sanguineus (21.9%), Hyalomma detritium (25%), Hyalomma marginatum (2.4%), Hyalomma anatolicum (0.8%), Hyalomma asiaticum (1.6%), Hyalomma dromedarii (43). %) and other Hyalomma (4.7%). The presence of virus was confirmed in 15.9% of the samples sent to the laboratory by reverse polymerase chain reaction (RT-PCR). The virus was observed in Rhipicephalus sanguineus, Hyalomma detritium and Hyalomma asiaticum. The most infected hosts were sheep and goats, and the ticks caught were all from lowland areas. The villages of this region can be considered endemic for CCHF. Therefore, it is recommended to follow controlling and monitoring policies in the region more carefully.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
75
83
https://nfvm.uoz.ac.ir/article_118317_6d7766416977a10f1d4323da78a9c4e6.pdf
dx.doi.org/10.22034/nfvm.2020.118317
A Review on Important Poultry Feed Mycotoxins, their Pathogenicity and Methods of Combating
ehsan
Oskoueian
Mashhad Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education, and Extension Organization (AREEO), Mashhad, Iran.
author
farough
kargar
PhD student in poultry nutrition, Ferdowsi University of Mashhad, Mashhad, Iran
author
hasan
kermanshahi
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
author
mahdi
salaripour
PhD Student in Animal Nutrition, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
author
text
article
2020
per
Mycotoxins are chemical compounds produced by fungi in the agricultural products that pose a serious threat to animals and humans health. The most important mycotoxins are aflatoxin B1, deoxynivalenol, zearalenone, ochratoxin a, T2 toxin, and fumonisin B1. The presence of these mycotoxins in animal diets could impair feed conversion ratio, mortality rate, increased production costs, and ultimately reduced production efficiency in the poultry industry. Methods of inhibiting mycotoxins are including dietary application of mineral adsorbents, organic adsorbents, microorganisms, microbial metabolites, and plant bioactive compounds in individual or combination forms. These compounds inhibit the myctoxins through chelationg, degradation, biotransformation, or enhancing the liver function and the immune system against mycotoxins toxicity. Due to the differences in the chemical nature of each mycotoxins, considering the different inhibitory mechanisms in mycotoxin inhibitors in mandatory. Hence, the use of broad-spectrum mycotoxins inhiboitors seems to be necessary to eliminate wide variety of mycotoxins in poultry diets.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
84
104
https://nfvm.uoz.ac.ir/article_122887_2803507478888f63ed241c2ea2e74731.pdf
dx.doi.org/10.22034/nfvm.2020.122887
The effect of intrauterine administration of Zataria multiflora ex-tract in cows with clinical endometritis on the incidence of abortion in subsequent pregnancy
abolfazl
hajibemani
1 - Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
author
text
article
2020
per
Endometritis is one of the most common uterine diseases postpartum. Arcanobacterium pyogenes, Escherichia coli, Fusobacterium necrophorum and Prevotella melaninogenicus bacteria are that correlated with endometritis. Zataria (Z) multiflora extract has antibacterial effect that has been used for treatment of endometritis in cows. But it may have adverse effects on the fetus. The objective of the present study was to determine the abortion effects of Z multiflora extract in pregnant cows that improved from endometritis before pregnancy using Z multiflora extract. 48 pregnant dairy cows with a history of clinical endometritis that improved and became pregnant following intrauterine infusion of placebo (n = 6), penicillin + streptomycin (n = 19) and Z multiflora extract (n = 23) were included in this study. Evaluation of pregnancy of treatment groups was performed using ultrasonography from 30 days of gestation to calving. Abortions were recorded in treatment groups. The data was statistically analyzed using the SPSS software .values of p≤ 0.05 was considered as significant data. The results of this study showed that the percentage of abortion was higher in Z. multiflora extract group(34.8%) compared to the control group (0%) and the penicillin + streptomycin group (10.5%), This difference was statistically very close to significant (P = 0.06). According to the results of the present study, intrauterine infusion of Z. multifloracan can increase the incidence of abortion in dairy cows, so it is to be more cautious in consuming of Z. multiflora.
New Findings in Veterinary Microbiology
University of Zabol
2645-4491
2
v.
2
no.
2020
105
115
https://nfvm.uoz.ac.ir/article_128001_080155ab0c435ee3f449f145b70c8873.pdf
dx.doi.org/10.22034/nfvm.2020.128001