New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Antibacterial effect of Ganoderma+silver nanocomplex extract on Strep-tococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii and Evaluation of nanocomplex tox-icity on Vero cell line

Document Type : Original Article

Authors
Faezeh Shokouhi 1
Majid Jamshidian-Mojaver 2
Hamidreza Farzin 2
Seyed-Elias Tabatabaeizadeh 2
Samira Kadoughani Sani 3
Mohadese Amiri 4
1 Graduated of Biotechnology, Faculty of Basic Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2 Assistant professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad Branch, Mashhad, Iran
3 Master of Microbiology, Faculty of Basic Sciences, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran
4 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad Branch, Mashhad, Iran
10.22034/nfvm.2022.339684.1140
Abstract
The aim of this study was to investigate the antibacterial effects of Ganoderma+silver extract complex nanocomplex on bacteria causing nosocomial infections including Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii. In the present study, silver nanoparticles were first loaded on Ganoderma extract. Then, tests such as FTIR and scanning electron microscopy were performed to confirm the loading of silver nanoparticles on Ganoderma extract. Then, the effects of nanocomplexes on Vero cell line were investigated to determine the toxicity of this compound on the cell. Also, the antibacterial properties of nanocomplexes of Ganoderma+silver extract on the studied bacteria were investigated. The MIC results of the fungus+silver nanocomplex were observed on Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes and Acinetobacter baumannii at 875, 218.75, 109.375 and 54.68, respectively. The results of this study showed that this complex has no toxic effect on eukaryotic cell line. Therefore, the synthesis of Ganoderma+silver nanocomplex can be used in medicine and food industry as an innovative, economical and environmental antimicrobial agent.
Keywords
Ganoderma fungus
silver nanoparticles
antibacterial properties
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  • Receive Date 30 April 2022
  • Accept Date 22 June 2022