New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Antimicrobial prevalence of Listeria monocytogenes and determination of the frequency of virulence genes in Red meat types sold in Chaharmahal and Bakhtiari province, Iran

Document Type : Original Article

Authors
1 PhD student, Department of Food Hygiene, Shahrekord branch, Islamic Azad University, Shahrekord, Iran
2 Professor, Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 Assistant Professor, Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract
Listeria monocytogenes is a cold-loving pathogen that can grow at low temperatures and causes listeriosis. Listeriosis is characterized by a low prevalence but a high mortality rate, and its resistance to antimicrobial compounds has become a significant challenge. The aim of the present study was to investigate the prevalence of antibiotic resistance and virulence genes in Listeria monocytogenes strains found in meat sold in the Chaharmahal and Bakhtiari province of Iran. In this research, 300 raw meat samples (from cow, buffalo, goat, sheep, and camel) were randomly collected from supply centers in Chaharmahal and Bakhtiari provinces. The results showed that out of the 300 samples, 138 (46%) were infected with Listeria monocytogenes. The infection rates were as follows: 12.23% in buffalo meat, 9% in sheep, 14% in cow, 6.33% in camel, and 4.33% in goat. PCR results indicated the presence of inl A genes in 43.33% of the samples, inl B in 18.33%, plcA in 26.66%, and plcB in 11.67%. The antibiotic resistance results showed that the highest resistance was against gentamicin (81.15%) and penicillin (78.26%), while the lowest resistance was observed for sulfamethoxazole (45.66%) and olendamycin (44.20%).  According to the findings of this study, raw meat is an important reservoir for the transmission of Listeria monocytogenes strains with virulence and antibiotic resistance genes. Therefore, maintaining sanitary conditions in animal husbandry and slaughterhouses is essential to controlling the prevalence of Listeria monocytogenes, which would help reduce the incidence of strains carrying virulence and antibiotic resistance genes.
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