New Findings in Veterinary Microbiology

New Findings in Veterinary Microbiology

Molecular epidemiology, genotyping and phylogenetic study of bovine norovirus from diarrheic calves in Iran

Document Type : Original Article

Authors
1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran
2 Razi vaccine and serum research institute, Agriculture Research Education and Extension Organization (AREEO), Karaj, Iran
Abstract
Norovirus is a highly contagious foodborne pathogen associated with calf diarrhea, causing economic losses in dairy cattle herds. This virus can infect a wide range of species, including cattle, pigs, dogs, cats, mice, sheep, lions, and humans. In this study, the prevalence and genetic sequencing of bovine norovirus as a causative agent of gastroenteritis in calves in Iran were investigated. A total of 163 fecal samples were collected from diarrheic calves under two months of age across five provinces in Iran during 2023–2024 and analyzed using RT-PCR with two pairs of primers. Two positive samples were sequenced for phylogenetic analysis (GenBank accession numbers: SUB13053245 and SUB13053259). RT-PCR results indicated that 68 samples (41%) tested positive for bovine norovirus genogroup III. Additionally, a higher prevalence of norovirus was observed in colder seasons (autumn and winter). Phylogenetic analysis revealed that sample SUB13053245 exhibited a close genetic relationship (97% similarity) with bovine norovirus strains reported from East Asia and Egypt, while sample SUB13053259 clustered with norovirus strains from European countries such as Belgium, France, and the United Kingdom. These findings highlight the high prevalence of bovine norovirus genogroup III in diarrheic calves in Iran, emphasizing the need for further investigations, particularly within the One Health framework, to assess the molecular epidemiology and risk factors associated with norovirus infections in Iran.
Keywords
Subjects

1- Glass RI, Parashar UD, Estes MK.
Norovirus gastroenteritis. N Engl J Med. 2009;
361(18): 1776-85.
2- Di Felice E, Mauroy A, Dal Pozzo F, Thiry
D, Ceci C, Di Martino B, et al. Bovine noroviruses:
a missing component of calf diarrhoea diagnosis.
Vet J. 2016; 207: 53-62.
3- Ushijima H, Fujimoto T, Müller WE,
Hayakawa S. Norovirus and foodborne disease: a
review. Food Saf (Tokyo). 2014; 2(3): 37-54.
4- Jung K, Scheuer KA, Zhang Z, Wang Q,
Saif LJ. Pathogenesis of GIII.2 bovine norovirus,
CV186-OH/00/US strain in gnotobiotic calves. Vet
Microbiol. 2014; 168(1): 202-7.
5- Otto PH, Clarke IN, Lambden PR, Salim
O, Reetz J, Liebler-Tenorio EM. Infection of
calves with bovine norovirus GIII.1 strain Jena
virus: an experimental model to study the
pathogenesis of norovirus infection. J Virol. 2011;
85(22): 12013-21.
6- Villabruna N, Koopmans MP, de Graaf M.
Animals as reservoir for human norovirus. Viruses.
2019; 11(5): 478.
7- Castells M, Caffarena RD, Casaux ML,
Schild C, Castells F, Castells D, et al. Detection,
risk factors and molecular diversity of norovirus
GIII in cattle in Uruguay. Infect Genet Evol. 2020;
86: 104613.
8- Gülacti I, Sözdutmaz I, Işıdan H. Molecular
characterization of the bovine noroviruses from
diarrheic calves in Turkey. Turk J Vet Anim Sci.
2016; 40(4): 428-33.
9- Guo Z, He Q, Yue H, Zhang B, Tang C. First
detection of Nebovirus and Norovirus from cattle in
China. Arch Virol. 2018; 163: 475-8. 2010; 166(26):
818-822.
10- Berchtold JF, Constable PD. Antibiotic
treatment of diarrhea in preweaned calves. Food
Anim Pract. 2009; 520.
11- Bartels CJ, Holzhauer M, Jorritsma R,
Swart WA, Lam TJ. Prevalence, prediction and
risk factors of enteropathogens in normal and non-
normal faeces of young Dutch dairy calves. Prev Vet
Med. 2010; 93(2-3): 162-9.
12- Izzo M, Kirkland P, Mohler V, Perkins N,
Gunn A, House J. Prevalence of major enteric
pathogens in Australian dairy calves with diarrhoea.
Aust Vet J. 2011; 89(5): 167-73.
13- Castells M, Colina R. Viral enteritis in
cattle: to well known viruses and beyond. Microbiol
Res. 2021; 12(3): 663-82.
14- Mesquita JR, Costantini VP, Cannon JL,
Lin S, Nascimento MSJ, Vinjé J. Presence of
antibodies against genogroup VI norovirus in
humans. Virol J. 2013; 10: 1-5.
15- Mauroy A, Scipioni A, Mathijs E, Saeger-
man C, Mast J, Bridger JC, et al. Epidemiological
study of bovine norovirus infection by RT-PCR and
a VLP-based antibody ELISA. Vet Microbiol. 2009;
137(3-4): 243-51.
16- Hutson AM, Atmar RL, Estes MK.
Norovirus disease: changing epidemiology and host
susceptibility factors. Trends Microbiol. 2004;
12(6): 279-87.
17- Vinjë J. Advances in laboratory methods
for detection and typing of norovirus. J Clin
Microbiol. 2015; 53(2): 373-81.
18- Scipioni A, Mauroy A, Vinje J, Thiry E.
Animal noroviruses. Vet J. 2008; 178(1): 32-45.
19- Wolf S, Williamson WM, Hewitt J, Ri-
vera-Aban M, Lin S, Ball A, et al. Sensitive
multiplex real-time reverse transcription-PCR assay
for the detection of human and animal noroviruses
in clinical and environmental samples. Appl Environ
Microbiol. 2007; 73(17): 5464-70.
20- Cho YI, Kim WI, Liu S, Kinyon JM, Yoon
KJ. Development of a panel of multiplex real-time
polymerase chain reaction assays for simultaneous
detection of major agents causing calf diarrhea in
feces. J Vet Diagn Invest. 2010; 22(4): 509-17.
21- Wolf S, Hewitt J, Greening GE. Viral
multiplex quantitative PCR assays for tracking
sources of fecal contamination. Appl Environ
Microbiol. 2010; 76(5): 1388-94.
22- Cui Y, Chen X, Yue H, Tang C. First
detection and genomic characterization of bovine
norovirus from yak. Pathog. 2022; 11(2): 192.
23- Richards GP, Watson MA, Fankhauser
RL, Monroe SS. Genogroup I and II noroviruses
detected in stool samples by real-time reverse
transcription-PCR using highly degenerate universal
primers. Appl Environ Microbiol. 2004; 70(12):
7179-84.
24- Pinior B, Firth CL, Richter V, Lebl K,
Trauffler M, Dzieciol M, et al. A systematic review
of financial and economic assessments of bovine
viral diarrhea virus (BVDV) prevention and
mitigation activities worldwide. Prev Vet Med.2017; 137: 77-92.
25- Mischenko VA, Mischenko AA, Nikeshina
TB, Petrova ON, Brovko YV, Kushlubaeva AI.
The problem of norovirus infection in animals
(literature review). Vet Sci Today. 2024; 118.
26- Farahmand M, Moghoofei M, Dorost A,
Shoja Z, Ghorbani S, Kiani SJ, et al. Global prev-
alence and genotype distribution of norovirus infec-
tion in children with gastroenteritis: a meta-analysis
on 6 years of research from 2015 to 2020. Rev Med
Virol. 2022; 32(1): e2237.
27- Robilotti E, Deresinski S, Pinsky BA.
Norovirus. Clin Microbiol Rev. 2015; 28(1): 134-
64.
28- Pourasgari F, Kaplon J, Sanchooli A,
Fremy C, Karimi-Naghlani S, Otarod V, et al.
Molecular prevalence of bovine noroviruses and
neboviruses in newborn calves in Iran. Arch Virol.
2018; 163(5): 1271-7.
29- Karayel-Hacioglu I, Alkan F. Molecular
characterization of bovine noroviruses and
neboviruses in Turkey: detection of recombinant
strains. Arch Virol. 2019; 164: 1411-7.
30- Klein-Jöbstl D, Iwersen M, Drillich M.
Farm characteristics and calf management practices
on dairy farms with and without diarrhea: a case-
control study to investigate risk factors for calf
diarrhea. J Dairy Sci. 2014; 97(8): 5110-9.
Volume 8, Issue 4
Winter
Winter 2026 Article ID:10.22034/nfvm.2025.515517.1279

  • Receive Date 07 April 2025
  • Revise Date 07 June 2025
  • Accept Date 08 June 2025