Investigating the rate of pouring of beef produced in Zahedan to Escherichia Coli using MPN and detection of O157:H7 by PCR

Document Type : Original Article

Authors

1 1- Department of pathobiology, Faculty of Veterinary Medicine, University of Zabol

2 Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Sistan and Baluchistan, Iran

10.35066/J040.2019.478

Abstract

Beef contamination usually occurs in the killing process. One of the intestinal bacterial pathogen that causes disease in human and animals everywhere is bacterial pathogen E. coli. One of the important serotypes of this bacterium is E. coli O157: H7. The aim of this study was to determine the rate of contamination of beef produced in Zahedan to E. coli by MPN Method and also O157:H7 strains isolated by PCR. During this study, conducted within six months from July 1396 to July 1397, 360 beef samples from cattle meat packages of Zahedan City, from different parts of the carcasses isolates of the heat-resistant E. coli in the laboratory was carried out using the MPN method. Seventy isolates were finally detected. The final test was performed using a specific primer as rfb to detect E. coli O157:H7 using a PCR reaction. Only one isolate was confirmed based on this serotype, which indicates the proper health status of the meat produced in Zahedan. In the present study, a molecular study of 2 isolates of heat-resistant Escherichia coli bacteria was done. In the study of rfb gene, only one isolate in terms of Escherichia coli serotype 7H: 157O was confirmed. With this study, the presence of this bacterium in beef samples is confirmed, which can indicate that the presence of this bacterium in other raw animal products in this province should also be investigated and tracked.

Keywords

References

1- Jafareyan-Sedigh M, Rahimi E, Doosti A. Isolation of Escherichia coli O157: H7 in sheep meats using cultural and PCR method,  Shahrekord Univ Med Sci. 2011; 13(2): 61-68. [In Persian[
2- Jamshidi A, Bassami M.R, Rasooli M. Isolation of Escherichia coli O157:H7 from ground beef samples collected from beef markets, using conventional culture and polymerase chain reaction in Mashhad, northeastern Iran, IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR). 2008; 9(22): 72-76.
3- RAJABZADEH S, BAHREINI M, SHARIFMOGHADAM M.R. Simultaneous detection of foodborn pathogenes Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella spp. By Multiplex PCR in ready–to–eat vegetables, Iranian Food Science and Technology Research Journal;2017 ; 13(1): 105- 111. [In Persian[
4- Koohdar V.A. Study of Beef Carcass Bacterial Contamination in Karajrak Slaughterhouse , Journal of Food Hygiene. 2013; 3(10): 43-51. [In Persian[
5- Safarpoor F, Rahimi A, ghobadi M, Yahaghi, A. Escherichia coli producing shigatoxin in sheep cheese, IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE. 2013; 19(66): 25-29. [In Persian[
6- Noroozi J. Practical Methods for Identifying Bacteria , 1nd ed. Tehran: Hayan Publications; 2010.
7- Beneduce L, Spano G, Massa S. Escherichia coli O157:H7 general characteristics, isolation and identification techniques. Annals of Microbiology. 2003; 53(4): 511-527.
8- Kim J, Kim S, Kwon N, Bae W, Lim J, Koo H. and et. al. Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD. J. Vet. Sci. 2005; 6(1): 6-19.
9- Marshall K.M. Identification of Escherichia coli O157: H7 meat processing indicators for fresh meat through comparison of the effects of selected antimicrobial interventions. Journal of food protection.2005; 68(12): 2580-2586.
10- Nikbakht B, Sani A. Identification Of Salmonella sp. From Contaminated Meat Samples by MultiplexnPCR-BASED Assay.  Journal of Experimental Biology and Agricultural Sciences. 2016; 4(5): 513-518
11- Ashgan M, Abdullah A. A, Adel M, Jakeen K. J, Onizan G. Z, Hassan A. and et al. Molecular characterization of Escherichia coli O157:H7 recovered from meat and meat products relevant to human health in Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences. 2015; 22(6): 725–729.
12- Korbandeh M, Emadi M, Hoseinizadeh ghasemi A, saiahi A. Isolation of  Escherichia coli O157: H7 from cattle meat in slaughter house of Kazeroon  By PCR. 12th Iranian Veterinary students Congress 04-05 September 2018, Semnan University-Iran. [In Persian[

Files

History

  • Receive Date: 04 January 2020
  • Accept Date: 06 January 2020

Share

How to cite

Statistics