Investigation of the effect of fungal and copper nanocomplexes on bacte-ria causing nosocomial infections

Document Type : Original Article

Authors

1 Master of Microbiology, Department of Biotechcology, Sabzevar Branch, Islamic Azad University of Sabzevar, Sabzevar, Iran.

2 Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran.

3 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran.

Abstract

Nosocomial infection is considered as one of the major and most basic medical problems in all hospitals and affects an average of 5 to 10% of hospitalized patients. Exacerbating factors of nosocomial infections include frequent use of tools as well as repeated contacts with medical staff. In this study, the antibacterial effects of copper nanoparticles using aqueous extract of Ganoderma lucidum on standard strains of Staphylococcus aureus, Acinetobacter baumannii and Streptococcus pyogenes and Pseudomonas aeruginosa Which are the cause of nosocomial infections were investigated. electron microscopy was used to measure the dimensions and shape of copper nanoparticles. Infrared spectroscopy was also performed to investigate possible organic compounds that could be involved in the synthesis of nanoparticles. Also, to observe the effectiveness of this nanocomplex, MTT test was performed on two vero cell lines and L 929 fibroblast cell line. The results of scanning electron microscopy in this study showed that the sample of the extract in morphology is completely uniform. Copper nanoparticles are spherical and in the range of 20-30 nm. Also, in the study of mtt test, ANOVA test showed that the value of Sig is zero, which indicates a significant difference between different concentrations and positive and negative control samples. According to the results of this study, fungal and copper nanocomplexes had a relatively strong antibacterial effect against bacteria that cause nosocomial infections. The non-toxicity proposal of copper nanoparticles should be investigated in laboratory spaces in order to use the antibacterial properties of these nanoparticles in human applications.

Keywords

References

1- Ghotbi F, Raghibmotlagh M, Valaie N. Nosocomial sepsis in NICU department in Taleghani hospital, 2001-2002. Research in Medicine. 2005 Dec 10; 29(4): 313-7. [In Persian].
2- Bienve nido D,Alora, M.D, Manuel B, Zacarias M.D, et al.,Nosocamial Infection in santo Tomas university Hospital. J Microbiol Infect Dis1984; 13(1):36-48.
3- Berenholtz S.M, Pronovost P.J, Lipsett P.A, et al, Eliminating catheter-related bloodstream infections in the intensive care unit. Crit Care Med 2004; 32(10):2014–2020.
4- Emilia Ma, Baleva A, Adrian C. Catheter-Related Intravascular Infections in Critical Care Units. Infect Diseases & Tropical Med 1990; 26(2): 251-54.
5- Amiri M, Jajarmi M, Ghanbarpour R. Prevalence of resistance to quinolone and fluoroquinolone antibiotics and screening of qnr genes among Escherichia coli isolates from urinary tract infection. Int J Enteric Pathog. 2017;5(4):100-5. [In Persian].
6- Roy N, and Barik A. Green Synthesis Of Silver Nanoparticles From The Unexploited Weed Resources. IJNT 2010; 4: 95–101.
7- Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ. Antimicrobial effects of silver nanoparticles. J Nanomedicine. 2007; 3(1): 95-101.
8- Ketabchi M, Iessazadeh Kh, Massiha A. Evaluate the inhibitory activity of ZnO nanoparticles against standard strains and isolates of Staphylococcus aureus and Escherichia coli isolated from food samples. JFM 2017; 4(1): 63-74.
9- Mosmann,T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. Immunol. Methods; 65:55-63.
10- Mayeur, S., Spahis, S., Pouliot, Y., & Levy, E. (2016). Lactoferrin, a pleiotropic protein in health and disease. Antioxidants & redox signaling, 24(14), 813-836.
11- Yousefi E, Rafienia M, Fazeli H, Zaman Kasai M. In-Vitro Effects of Copper Nanoparticles on Common Bacterial Strains Implicated in Nosocomial Infections. J Isfahan Med Sch 2013; 31(240): 830-42. [In Persian].
12- Ruparelia JP, Chatterjee AK, Duttagupta SP, Mukherji S. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater 2008; 4(3): 707-16.
13- Dehghan Nayeri, F., Mirhosseini, M., Mafakheri, S., Zarrabi, M. Antibacterial and antifungal effects of silver nanoparticles synthesized by the aqueous extract of sesame (Sesamum indicum L.). Journal of Cellular and Molecular Research (Iranian Journal of Biology), 2018; 31(1): 16-26. [In Persian].
14- Mafakheri, S., Dehghan Nayeri, F., Mirhosseini, M. study the biological production and antibacterial and antifungal effects of silver nanoparticles synthesized by the methanolic extract of clove (Syzygium aromaticum). JMBS. 2017; 8 (2) :93-103. [In Persian].
15- Shaffiey SF, Ahmadi M, Shaffiey SR, Shapoori M, Varshoie H, Azari F. Synthesis of copper oxide (CuO) nanoparticles and surveying its bactericidal properties against Aeromonas Hydrophila bacteria. Journal of Fasa University of Medical Sciences. 2015; 5(1):36-43. [In Persian].
New Findings in Veterinary Microbiology
Volume 3, Issue 1 - Serial Number 1
September 2020
Pages 39-46

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History

  • Receive Date: 27 October 2020
  • Accept Date: 20 December 2020

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