Determination of the prevalence of Streptococcus equi subspecies zooepi-demicus In aborted fetuses of Mare by Molecular Method

Document Type : Original Article

Authors

1 Graduated of Veterinary Medicine Faculty, Shahrekord Branch, Islamic Azad University, ShahreKord, Iran

2 Assistance Professor in Clinica Pathology Department, Veterinary Medicine Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Abstract

Abortion in domestic animals has always been considered as one of the problems of the livestock industry in all parts of the world, whose causes are numerous and varied. Streptococcus equi subspecies zooepidemicus is a gram positive coccus and negative catalase which is one of the most important causes of abortion and loss of  foal during pregnancy in mare. The aim of this study was to investigate the presence of  DNA of this bacteria in a number of mare abortion fetuses  in western provinces of Iran. For this purpose, 125 samples of aspirated stomach contents of the aborted fetuses were tested by PCR method to detect the sodA gene. The results of this study showed that 23 (18.4%) of 125 aborted fetuses were infected with Streptococcus equi subspecies zooepidemicus. The findings of this study indicate a high incidence of  Streptococcusis  infection and showed that one of the most important factors in abortion in mares that control programs to reduce the economic losses of this bacteria in Iran is necessary. In this study, for the first time, a bacteria in the contents of the stomach of aborted fetuses of mares  were identified as One of the main causes of abortion in Iran.

Keywords

References

1- Hasani Tabatabaei A.H, Firouzi R. Bacterial Diseases of Livestock. University Of Tehran Press. 2005; 2(2): 29-49. ]In Persion[
2- Arthur G.H, Pierson H, Parkinson T.J. Veterinary Reproduction and Obstetrics; W.B. 8th.Ed. Saunders London; 2001, P: 601-650.
3- Alber J, El-Sayed A, Lammler C, Hassan A.A, Weiss R, Zschock M. Multiplex polymerase chain reaction for identification and differentiation of Streptococcusequi subsp. zooepidemicus and Streptococcus equi subsp.equi. J. Vet. Med. B. 2004; 51(10): 455-458.
4- Brinsko S.B, Blanchard T.L, Varner D.D, Schumbacher J, Love C.C, Hinrichs K, Hartman D. MANUAL OF EQUINE REPRODUCTION; 3rd.Ed. Mosby; 2011, P: 100-120.
5- Casagrande Proietti P, Bietta A, Coppola G, Felicetti M, Cook R.F, Coletti M, et al., Isolation and characterization of b-haemolytic-Streptococci fromendometritis in mares. Veterinary Microbiology. 2011; 152(1-2): 126–130.
6- Cordoni G.C, Williams A.W, Durham A, Florio D.F, Zanoni R.G , La Ragione R.M. Rapid diagnosis of strangles (Streptococcus equi subspecies equi) using PCR. Research in Veterinary Science. 2015; 102(2): 162-166.
7- Davies Morel M.C.G. Equine Reproductive Physiology, Breeding and Stud Management. 2nd.Ed. CABI Publishing; 2003, P: 263-295.
8- England G.C.W. Fertility and Obstetrics in the Horse.3rd.Ed. Blackwell Publishing; 2005, P: 170-177.
9- Erol E, Jackson C.J, Horohov D, Locke S, Smith J.S , Carter C.C. Elevated serum amyloid A levels in cases of aborted equine fetuses due to fetal and placental infections. Theriogenology. 2016; 86(4): 971-975.
10- Javed R, Taku A.K, Gangil R , Sharma R.K. Molecular characterization of virulence genes of Streptococcus equi subsp. equi and Streptococcus equi subsp zooepidemicus in equines. Veterinary World. 2016; 9(8): 875-881
11- Knottenbelt D.C, Pascoe R.R, Lopate C, Leblanc M.M. Equine Stud Farm Medicine and Surgery. 1st.Ed. Saunders London;2003, P: 191-203.
12- Levent Kocabiyik A, Sonmez  G, Ulgen M, Ozakin C, Kocakaya E, Alasonyalilar A. Abortion due to Streptococcus equi subspecies zooepidemicus in a Mare. Turk J Vet Anim Sci. 2005; 29(12): 937-940.
13- Medina L, Cruz-Va´zquez C, Quezada T, Morales  E , Garcı´a-Va´zquez  Z. Survey of Neospora caninum infection by nested PCR in aborted fetuses from dairy farms in Aguascalientes, Mexico. Veterinary Parasitology. 2006; 136(3-4): 187-191.
14- McKinnon A.O, Squires E.L, Vaala W.E , Varner D.D. Equine Reproduction. 2nd .Ed. Blackwell Publishing Ltd; 2011, P: 1963-1980.
15- Sambrook J, Fritsch E.F,  Maniatis T. Molecular cloning: a laboratory manual. 2nd. Ed. Cold  Spring Harbor  Laboratory  Press; 1989, P: 150-250
16- Szeredi L, Tenk M, Jánosi S, Pálfi V, Hotzel H, Sachse K, ET AL. A survey of equine abortion and perinatal foal losses in Hungary during a three-year period (1998-2000). Acta Veterinaria Hungarica. 2008; 56 (3): 353-367.
17- Van der kolk J.H, Veldhuis kroeze, E.J.B. Infectious Diseases Of  The Horse; Manson Publishing Ltd; 2013, P: 10-124.
New Findings in Veterinary Microbiology
Volume 3, Issue 1 - Serial Number 1
September 2020
Pages 60-67

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History

  • Receive Date: 05 January 2021
  • Accept Date: 24 January 2021

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