Antibiotic resistance phenotype study in Corinne bacterium pseudotuber-culosis isolates isolated from sheep with cheese lymphadenitis in Binalood city of Mashhad

Document Type : Original Article

Authors

1 Graduate of Biochemistry, Faculty of Science, Payame Noor University of Mashhad, Mashhad, Iran

2 Assistant Professor-Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran

Abstract

Cheese lymphadenitis is a very common skin infectious disease among sheep and goats around the world caused by the bacterium Corinne bacterium Pseudotuberculosis. The development of this disease among livestock always causes significant economic losses. In this study, 40 samples of abscesses in live animal cases and carcasses of animal slaughterhouses in Mashhad and Binalood were examined. To sample swollen and purulent lymph nodes, a sample of about 1 cc was collected from the swollen glands by a sample syringe and the sample containing the sample was transferred to the laboratory along with ice. The collected samples were transferred to BHI culture medium and after confirmation by tests such as hot staining, catalase test and detection from bacterial strain, discing was performed to determine the resistance and sensitivity of the isolates by Kirby Baer method. Examination of the sensitivity and resistance of the studied isolates showed that the highest resistance of the isolates to Doxycycline antibiotics (72/5%) and the lowest resistance to Trimethoprim/Sulfamethoxazole antibiotics (17.5%). Also, in the study of the sensitivity of isolates, the highest sensitivity of isolates to antibiotics was enrofloxacin and the lowest was Chloramphenicol. The agar diffusion disc method can be used as a primary and primary screening method to determine the level of resistance and sensitivity, so a genotypic method can be used to accurately assess the resistance of the isolates.
 
 
 






Keywords

References

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New Findings in Veterinary Microbiology
Volume 3, Issue 1 - Serial Number 1
September 2020
Pages 88-95

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History

  • Receive Date: 13 February 2021
  • Accept Date: 17 February 2021

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