Molecular detection of Infectious Bronchitis virus in broiler chicken flocks

Document Type : Original Article

Authors

1 Assistant Professor, Department of poultry vaccines research and production, Razi Vaccine and Serum Research Institute, Agricultural research, Education and Extension organization, Karaj,Iran

2 Assistant Professor, Department of Avian diseases research and diognostic, Razi Vaccine and Serum Research Institute, Agricultural research, Education and Extension organization, Ka-raj,Iran

Abstract

Infectious bronchitis disease is one the most important diseases in poultry industry worldwide. Although vast vaccination with different vaccines the virus causes major economic losses in commercial poultry production. The main object of this study was to detect infectious bronchitis virus infection in respiratory complex in broiler chicken flocks. Tissue samples from 35 flocks in central and south regions of Iran in 2018 and 2019 were collected and investigated by RT-PCR reaction. For phylogenetic analysis, Nucleotide sequence of partial S1 gene was amplified then evaluated. Totally from 35 flock with vaccination history against IBV and suffering from respiratory problems15 flocks were positive for IBV infection (42/85 percent). Two genotype was detected including GI-23 Is/1494 like (variant2) and Mass type ones. IS/1494 like viruses isolated in this study were clustered with other Variant2 viruses reported from Iran which has increasing trend recent years. Massachussete viruses were not clustered with Iranian mass type viruses including vaccinal strains and had some differences. Thereafter result of this study indicates that even with frequent vaccination, IBVs can  cause outbreaks and severe problems in vaccinated chicken flocks.

Keywords

References

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New Findings in Veterinary Microbiology
Volume 3, Issue 2
April 2021
Pages 118-126

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History

  • Receive Date: 26 June 2021
  • Revise Date: 12 October 2021
  • Accept Date: 26 October 2021

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