Study on Sara-CoV 2 reserviors by phylogenetic analysis of aminoacid Sequences of S and Angiotension-converting Enzyme (ACE2) proteins

Document Type : Original Article

Author

Associate Professor, Department of Microbiology, Faculty of Basic Sciences, Kazeroon Branch, Islamic Azad Universi-ty, Kazeroon, Iran

Abstract

Sars-Cov2 has spherical shape with many spike proteins viz, M,N,E and S. S protein of this virus has major role in attachement of the virus to host receptor Angiotension-converting Enzyme (ACE2) protein.The purpose of this study was evaluation of Sars-Cov2 reservoirs by phylogenetic analysis aminoacid sequences of S and Angiotension-converting Enzyme (ACE2) proteins in the differents animals. To perform the study whole genome and amino acid sequences of S protein of Sars-Cov2 and sequences of ACE2 protein of some animals and human were downloaded from NCBI and GenBank and subjected to phylogenetic analysis of their relationship using MegaX soft ware. The results obtained indicated that all Sars-Cov2 isolated from different geographical areas have similar origin. On the other hand, amino acid profile of S protein in the different viral variants showed several sequences. In addition, amino acid sequence of Human ACE2 was very close to camel, Swine and bats (Pteropus vampyrus and Cynoplerus phinx). Therefore the present study showed that gene mutation in surface protein coding sequence of corona virus and the similarity of ACE2 amino acid sequences in different animals cause emerging the new variants of corona virus in the different reservoirs. Hence it might increase the rate of transmission of the virus to human even after vaccination and herd immunity.

Keywords

References

1- Singhal T. A review of coronavirus disease-2019 (COVID-19). The indian journal of pediatrics. 2020; 87(4): 281-6.
2- Li F. Structure, function, and evolution of coronavirus spike proteins. Annual review of virology. 2016; 3: 237-61.
3- Jung BK, An YH, Jang JJ, Jeon JH, Jang SH, Jang H. The human ACE-2 receptor binding domain of SARS-CoV-2 express on the viral surface of the Newcastle disease virus as a non-replicating viral vector vaccine candidate. PloS one. 2022; 17(2): e0263684.
4- Goyal M, Tewatia N, Vashisht H, Jain R, Kumar S. Novel corona virus (COVID-19); Global efforts and effective investigational medicines: A review. Journal of Infection and Public Health. 2021; 14(7): 910-21.
5- Koleya T, Kumara M, Goswami A, Ethayathulla AS, Hariprasada G. Structural modeling of Omicron spike protein and its complex with human ACE-2 receptor: Molecular basis for high transmissibility of the virus. Biochemical and biophysical research communications. 2022.
6- Alfaleh MA, Zawawi A, Al-Amri SS, Hashem AM. David versus goliath: ACE2-Fc receptor traps as potential SARS-CoV-2 inhibitors. InmAbs. 2022; 14(1): 2057832. Taylor & Francis.
7- Wang X, Ye Y, Gong H, Wu J, Yuan J, Wang S, et al. The effects of different angiotensin II type 1 receptor blockers on the regulation of the ACE-AngII-AT1 and ACE2-Ang (1–7)-Mas axes in pressure overload-induced cardiac remodeling in male mice. Journal of molecular and cellular cardiology. 2016; 97: 180-90.
8- Ye ZW, Yuan S, Yuen KS, Fung SY, Chan CP, Jin DY. Zoonotic origins of human coronaviruses. International journal of biological sciences. 2020; 16(10): 1686.
9- Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution. 2018; 35(6): 1547.
10- Boni MF, Lemey P, Jiang X, Lam TT, Perry BW, Castoe TA, et al. Evolutionary origins of the SARS-CoV-2 sarbecovirus lineage responsible for the COVID-19 pandemic. Nature microbiology. 2020; 5(11): 1408-17.
11- Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 2003; 426(6965): 450-4.
12- Zhou H, Chen X, Hu T, Li J, Song H, Liu Y, et al. A novel bat coronavirus closely related to SARS-CoV-2 contains natural insertions at the S1/S2 cleavage site of the spike protein. Current biology. 2020; 30(11): 2196-203.
13- Yang XL, Hu B, Wang B, Wang MN, Zhang Q, Zhang W, et al. Isolation and characterization of a novel bat coronavirus closely related to the direct progenitor of severe acute respiratory syndrome coronavirus. Journal of virology. 2015; 90(6): 3253-6.
14- Ren W, Qu X, Li W, Han Z, Yu M, Zhou P, et al. Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin. Journal of virology. 2008; 82(4): 1899-907.
15- Menachery VD, Graham RL, Baric RS. Jumping species—a mechanism for coronavirus persistence and survival. Current opinion in virology. 2017; 23: 1-7.
16- Luk HK, Li X, Fung J, Lau SK, Woo PC. Molecular epidemiology, evolution and phylogeny of SARS coronavirus. Infection, Genetics and Evolution. 2019; 71: 21-30.
17- Zarehzadeh S, Baserisalehi M. SARS-CoV-2 originated from laboratory?. Japanese Journal of Gastroenterology and Hepatology. 2020 Oct.1.
18- Yu P, Hu B, Shi ZL, Cui J. Geographical structure of bat SARS-related coronaviruses. Infection, Genetics and Evolution. 2019; 69: 224-9.
19- Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. nature. 2020; 579(7798): 270-3.
New Findings in Veterinary Microbiology
Volume 4, Issue 2
February 2022
Pages 111-119

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History

  • Receive Date: 02 March 2022
  • Revise Date: 08 April 2022
  • Accept Date: 30 April 2022

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