Theileriosis and Anaplasmosis are very prevalent in native ruminants of Sistan region of Iran. Bovine babesiosis could be found with fewer prevalence. Also, Trypanosoma evansi induce the Surra disease in the camels of the region. The objective of present study is study and comparison of common available methods in identification of such infective organisms including clinical appearance, giemsa staining and molecular techniques (2013-2018). The blood specimens of 636 cattle, 80 sheep and 213 camels have been investigated with giemsa staining and molecular methods. Also, the clinical features were evaluated. The current results indicated that molecular PCR amplification is a more sensitive tool as compared to smear scanning for the detection of blood protozoa. However, it doesn’t mean that molecular techniques could be considered more preferable. The advantages and disadvantages of each identification method is discussed comprehensively based on the type of the parasites, hosts and economical condition in the area.
Ganjali M, Dabirzadeh M, Sargolzaie M. Species diversity and distribution of ticks (Acari: Ixodidae) in Zabol county, eastern Iran. Journal of Arthropod Borne Disease. 2014; 8(2): 219-223.
Soosaraei M, Haghi MM, Etemadifar F, Fakhar M, Teshnizi SH, Hezarjaribi HZ, et al. Status of theileriosis among herbivores in Iran: A systematic review and meta-analysis. Veterinary World. 2018; 11(3): 332-341.
Zarei F, Ganjali M, Nabavi R. Identification of Theileriaspecies in sheep and vector ticks using PCR method in Zabol, eastern Iran. Journal of Arthropod Borne Disease. 2019; 13(1): 76-82.
Fathi A. Molecular detection of anaplasma species in cattle of Sistan region. DVM Thesis. University of Zabol, Iran. 2018.
Sazmand A, Joachim A. Parasitic diseases of camels in Iran (1931-2017) - a literature review. Parasite. 2017; 24: 21.
Majidiani H, Nabavi R, Ganjali M, Saadati D. Detection of Theileria annulatacarriers in Holstein-Friesian (Bos taurus taurus) and Sistani (Bos taurus indicus) cattle breeds by polymerase chain reaction in Sistan region, Iran. Journal of Parasitic Disease. 2016; 40(4): 1184-1188.
Sharifi N, Ganjali M, Nabavi R, Saadati D. A study on prevalence and identification of ovine Theileriaand Babesia infection in Zabol using PCR method. Journal of Parasitic Disease. 2016; 40(4): 1535-1539.
Zangooie F, Ganjali M, Keighobadi M, Nabavi R. Molecular detection of Trypanosoma evansi based on ITS1 rDNA gene in Camelus dromedarius in Sistan Region, Iran. Tropical Biomedicine. 2018; 35(4): 1140-1147.
Nouri-Sadegh M. Study of the prevalence of Babesia bigemina and Babesia bovis in native cattle with anemia in veterinary clinics of Sistan zone. MS Thesis. University of Zabol, Iran. 2016.
Zeinali R. Molecular study of Anaplasma species in camels of Zahedan, Saravan -Iran. MS Thesis. University of Zabol, Iran. 2018.
Lempereur L, Beck R, Fonseca I, Marques C, Duarte A, Santos M, et al. Guidelines for the detection of Babesia and TheileriaVector Borne and Zoonotic Disease. 2017; 17(1): 51-65.
Esteve-Gasent MD, Rodríguez-Vivas RI, Medina RF, Ellis D, Schwartz A, Cortés Garcia B, et al. Research on integrated management for Cattle fever ticks and bovine babesiosis in the United States and Mexico: current status and opportunities for binational coordination.Pathogens. 2020; 9(11): 871.
Ma Q, Liu J, Li Z, Xiang Q, Wang J, Liu A, et al. Clinical and pathological studies on cattle experimentally infected with Theileria annulata in China. Pathogens. 2020; 9(9): 727.
Jalali SM, Jolodar A, Rasooli A, Darabifard A. Detection of Theileria lestoquardi cross infection in cattle with clinical theileriosis in Iran. Acta Parasitologica. 2016; 61(4): 756-761.
Khattak RM, Rabib M, Khan Z, Ishaq M, Hameed H, Taqddus A, et al. A comparison of two different techniques for the detection of blood parasite, Theileria annulata, in cattle from two districts in Khyber Pukhtoon Khwa Province (Pakistan). Parasite. 2012; 19(1): 91-5.
Nabavi, R. (2023). Comparison of Common Available Methods in Identification of Ruminant's Blood Protozoa, Sistan Region of Iran. New Findings in Veterinary Microbiology, 5(2), 109-116. doi: 10.22034/nfvm.2023.378279.1167
MLA
Reza Nabavi. "Comparison of Common Available Methods in Identification of Ruminant's Blood Protozoa, Sistan Region of Iran". New Findings in Veterinary Microbiology, 5, 2, 2023, 109-116. doi: 10.22034/nfvm.2023.378279.1167
HARVARD
Nabavi, R. (2023). 'Comparison of Common Available Methods in Identification of Ruminant's Blood Protozoa, Sistan Region of Iran', New Findings in Veterinary Microbiology, 5(2), pp. 109-116. doi: 10.22034/nfvm.2023.378279.1167
VANCOUVER
Nabavi, R. Comparison of Common Available Methods in Identification of Ruminant's Blood Protozoa, Sistan Region of Iran. New Findings in Veterinary Microbiology, 2023; 5(2): 109-116. doi: 10.22034/nfvm.2023.378279.1167
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