Genetic fingerprinting of Escherichia coli bacterial isolated from Kerman zoo animals using ERIC-PCR

Document Type : Original Article

Authors

1 DVM, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Assistant professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 Assistant professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

4 Professor, Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

Genetic fingerprinting of Escherichia coli strains in one habitat can significantly help our understanding of genetic diversity and circulation in different hosts. In this study, 48 stool samples were collected from 24 healthy zoo animal species in Kerman zoo by sterile swab. After the isolation of Escherichia coli, the strains were genetically fingerprinted by the ERIC-PCR method, and the bond patterns obtained from electrophoresis were calibrated and analyzed with Gel Quest software. In the next step, the similarity of ERIC templates was determined by drawing a phylogenetic tree by Cluster Viz software using the UPGMA algorithm. In this method, 22 clones were identified with a ≥95% similarity cutoff; The ERIC pattern of pigeon isolates had 100% similarity with a peacock isolate, and the ERIC pattern of ostrich isolates had 100% similarity with one peacock isolate. The isolates of horned chicken, casco, and one isolate of the tawny owl were grouped together with 100% similarity in terms of ERIC pattern. Also, camel isolates and one isolate from a tawny owl showed 100% similarity in the ERIC pattern. Considering that several strains isolated from different hosts with different species had 100% similarity in the ERIC pattern, it can be concluded that due to the proximity of different animal species in the same habitat such as a zoo, these strains circulated among different animals.

Keywords

Main Subjects

References

 
1- Bagheri M, Jajarmi M, Ghanbarpour R. Identification of colicinogenic Escherichia coli isolates from broiler car-casses and their inhibitory effect on E. coli pathotypes. New Find Vet Microbiol. 2023; 5(2): 31–40. [In Persion]
2- O’Boyle N, Douce GR, Farrell G, Rattray NJW, Schembri MA, Roe AJ, et al. Distinct ecological fitness factors coordinated by a conserved Escherichia coli regulator during systemic bloodstream infection. Proc Natl Acad Sci. 2023; 120(1): e2212175120.
3- Lorrayne de Souza AM, Motta RG, Martinez AC, Orsi H, Hernandes RT, Rall VLM, et al. Virulence-encoding genes related to extraintestinal pathogenic E. coli and multidrug resistant pattern of strains isolated from neonatal calves with different severity scores of umbilical infections. Microb Pathog. 2023; 174: 105861.
4- Burgaya J, Marin J, Royer G, Condamine B, Gachet B, Clermont O, et al. The bacterial genetic determinants of Escherichia coli capacity to cause bloodstream infections in humans. bioRxiv. 2023; 2012–22.
5- Abdlla YA, Al-Sanjary RA. The molecular identification of diarrheagenic Escherichia coli (DEC) isolated from meat and meat products. Iraqi J Vet Sci. 2023; 37(1): 9–15.
6- Askari A, Ghanbarpour R, Akhtardanesh B, Aflatoonian MR, Sharifi H, Jajarmi M, et al. Detection of zoonotic diarrheagenic pathotypes of Escherichia coli in healthy household dogs. Iran J Microbiol. 2020; 12(6): 522. [In Persion]
7- Jajarmi M, Fooladi AAI, Badouei MA, Ahmadi A. Virulence genes, Shiga toxin subtypes, major O-serogroups, and phylogenetic background of Shiga toxin-producing Escherichia coli strains isolated from cattle in Iran. Microb Pathog. 2017; 109: 274–9. [In Persion]
8- Jajarmi M, Askari Badouei M, Imani Fooladi AA, Ghanbarpour R, Ahmadi A. Pathogenic potential of Shiga toxin-producing Escherichia coli strains of caprine origin: virulence genes, Shiga toxin subtypes, phylogenetic background and clonal relatedness. BMC Vet Res. 2018; 14(1): 1–8. [In Persion]
9- Bender JB, Shulman SA. Reports of zoonotic disease outbreaks associated with animal exhibits and availability of recommendations for preventing zoonotic disease transmission from animals to people in such settings. J Am Vet Med Assoc. 2004; 224(7): 1105–9.
10- Sherwen SL, Hemsworth PH, Butler KL, Fanson K V, Magrath MJL. Impacts of visitor number on Kangaroos housed in free‐range exhibits. Zoo Biol. 2015; 34(4): 287–95.
11- Vale AP, Cousins C, Tzora A, McCarron M, Green A, Molloy S, et al. Molecular characterization of fecal escherichia coli isolated from zoo animals. J Zoo Wildl Med. 2020; 50(4): 813.
12- Schlager S, Lepuschitz S, Ruppitsch W, Ableitner O, Pietzka A, Neubauer S, et al. Petting zoos as sources of Shiga toxin-producing Escherichia coli (STEC) infections. Int J Med Microbiol. 2018; 308(7): 927–32.
13- Alsultan A, Elhadi N. Evaluation of ERIC-PCR method for determining genetic diversity among Escherichia coli isolated from human and retail imported frozen shrimp and beef. Int J Food Contam. 2022; 9(1): 12.
14- Ranjbar R, Tabatabaee A, Behzadi P, Kheiri R. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genotyping of Escherichia coli strains isolated from different animal stool specimens. Iran J Pathol. 2017; 12(1): 25. [In Persion]
15- Shnaiderman-Torban A, Steinman A, Meidan G, Paitan Y, Abu Ahmad W, Navon-Venezia S. Petting zoo animals as an emerging reservoir of extended-spectrum β-lactamase and ampC-producing Enterobacteriaceae. Front Microbiol. 2019; 10: 2488.
16- Ramazanzadeh R, Zamani S, Zamani S. Genetic diversity in clinical isolates of Escherichia coli by enterobacterial repetitive intergenic consensus (ERIC)-PCR technique in Sanandaj hospitals. Iran J Microbiol. 2013; 5(2): 126–31. [In Persion]
17- De Gregorio E, Silvestro G, Petrillo M, Carlomagno MS, Di Nocera PP. Enterobacterial repetitive intergenic consensus sequence repeats in yersiniae: Genomic organization and functional properties. J Bacteriol. 2005; 187(23): 7945–54.
18- Ishihara K, Hosokawa Y, Makita K, Noda J, Ueno H, Muramatsu Y, et al. Factors associated with antimicrobial-resistant Escherichia coli in zoo animals. Res Vet Sci. 2012; 93(2): 574–80.
19- De Witte C, Vereecke N, Theuns S, De Ruyck C, Vercammen F, Bouts T, et al. Presence of broad-spectrum beta-lactamase-producing Enterobacteriaceae in Zoo Mammals. Microorganisms. 2021; 9(4): 834.
20- Wang Y, He T, Han J, Wang J, Foley SL, Yang G, et al. Prevalence of ESBLs and PMQR genes in fecal Escherichia coli isolated from the non-human primates in six zoos in China. Vet Microbiol. 2012; 159(1–2): 53–9.
21- Markey B, Leonard F, Archambault M, Cullinane A, Maguire D. Clinical Veterinary Microbiology E-Book. Elsevier Health Sciences. 2013.
22- Versalovic J, Koeuth T, Lupski R. Distribution of repetitive DNA sequences in eubacteria and application to finerpriting of bacterial enomes. Nucleic Acids Res. 1991; 19(24): 6823–31.
23- Ibrahim DR, Dodd CER, Stekel DJ, Ramsden SJ, Hobman JL. Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm. FEMS Microbiol Ecol. 2016; 92(4).
24- Casarez EA, Pillai SD, Mott JB, Vargas M, Dean KE, Di Giovanni GD. Direct comparison of four bacterial source tracking methods and use of composite data sets. J Appl Microbiol. 2007; 103(2): 350–64.
25- Fuentes Castillo D, Navas Suárez PE, Gondim MF, Esposito F, Sacristán C, Fontana H, et al. Genomic characterization of multidrug‐resistant ESBL‐producing Escherichia coli ST58 causing fatal colibacillosis in critically endangered Brazilian merganser (Mergus octosetaceus). Transbound Emerg Dis. 2021; 68(2): 258–66.
New Findings in Veterinary Microbiology
Volume 6, Issue 1
September 2023
Pages 29-39

Files

History

  • Receive Date: 13 January 2023
  • Revise Date: 28 February 2023
  • Accept Date: 27 May 2023

Share

How to cite

Statistics