Study of the frequency of iutA, sitA and irp2 genes in Escherichia coli iso-lated from poultry colibacillosis in Tabriz city in 2021

Document Type : Original Article

Authors

1 MSc, Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran

2 Assistant Professor, Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran

Abstract

Escherichia coli is one of the most important pathogenic microbes in poultry. Avian pathogenic Escherichia coli isolates are capable of pathogenicity outside the intestinal environment. Colibacillosis is one of the most common diseases in the poultry industry, which causes a lot of economic losses every year. The aim of this research was to study of the frequency of iutA, sitA and irp2 genes in Escherichia coli isolated from poultry colibacillosis in Tabriz city in 2021. 100 samples of Escherichia coli isolated from poultry colibacillosis were phenotypically identified by biochemical and staining methods. Then, the frequency of sitA, iutA and irp2 genes in these isolates was investigated by molecular method. The results showed that the frequency of iutA, irp2 and sitA genes in the tested Escherichia coli samples were 88%, 84% and 66%, respectively. Also, 52% of the tested isolates contained all three mentioned genes. No negative samples were observed in terms of the presence of studied genes. In this research, the high frequency of iron absorption genes in Escherichia coli samples tested may indicate the high potential virulence of these isolates. Therefore, it is necessary to conduct more studies and evaluate the importance of such possibilities in the epidemiology of the disease in poultry.

Keywords

Main Subjects

References

1- Mellata M. Human and avian extraintestinal pathogenic Escherichia coli: infections, zoonotic risks, and antibiotic resistance trends. Foodborne Pathog Dis. 2013; 10(11): 916–932.
2- Russo T.A, Johnson J.R. Medical and economic impact of extraintestinal infections due to Escherichia coli: focus on an increasingly important endemic problem. Microbes Infect. 2003; 5(5): 449-456.
3- Johnson T.J, Wannemuehler Y, Doetkott C, Johnson S.J, Rosenberger S.C, Nolan L.K. Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool. J Clin Microbiol. 2008; 46(12): 3987- 3996.
4- Akond M.A, Hassan S, Alam S, Shirin M. Antibiotic resistance of Escherichia coli isolated from poultry and poultry environment of Bangladesh. Am J Environ Sci. 2009; 5(1): 47-52.
5- Dissanayake D.R, Wijewardana T.G, Gunawardena G.A, Poxton I.R. Distribution of lipopolysaccharide core types among avian pathogenic Escherichia coli in relation to the major phylogenetic groups. Vet Microbiol. 2008; 132(3-4): 355-363.
6- Gao Q, Wang X, Xu H, Xu Y, Ling J, Zhang D, et al. Roles of iron acquisition systems in virulence of extraintestinal pathogenic Escherichia coli: salmochelin and aerobactin contribute more to virulence than heme in a chicken infection model. BMC Microbiol. 2012; 12: 143.
7- Hajihosein-Tabrizi A, Habibi M, Tabasi M, Asadi Karam M.R. Distribution of genes encoding iron uptake systems among the Escherichia coli isolates from diarrheal patients of Iran. J Med Microbiol Infect Dis. 2018; 6(1): 25-30.
8- De Lorenzo V, Neilands J. Characterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli. J Bacteriol. 1986; 167(1): 350-5.
9- Hussein A.H, Ghanem I.A, Eid A.A, Ali M.A, Sherwood J.S, Li G, et al. Molecular and phenotypic characterization of Escherichia coli isolated from broiler chicken flocks in Egypt. Avian Dis. 2013; 57(3): 602-11.
10- Martinez J, Cercenado E, Baquero F. Aerobactin production and plasmid distribution in Escherichia coli clinical isolates. FEMS Microbiol Lett. 1989; 60(1): 41-44.
11- Hitchins A.D, Feng P, Watkins W.D, Rippey S.R, Chandler L.A. Bacteriological Analytical Manual. 8th ed. USA: FDA publication. 1998, P: 68-104.
12- Chen J, Su Z, Liu Y, Wang S, Dai X, Li Y, et al. Identification and characterization of class 1 integrons among Pseudomonas aeruginosa isolates from patients in Zhenjiang, China. Int J Infect Dis. 2009; 13(6): 717-721.
13- Schouler C, Schaeffer B, Brée A, Mora A, Dahbi G, Biet F, et al. Diagnostic strategy for identifying avian pathogenic Escherichia coli based on four patterns of virulence genes. J Clin Microbiol. 2012; 50(5): 1673–1678.
14- Johnson J.R, Delavari P, O’Bryan T.T, Smith K.E, Tatini S. Contamination of retail foods, particularly turkey, from community markets (Minnesota, 1999–2000) with antimicrobial-resistant and extraintestinal pathogenic Escherichia coli. Foodborne Pathog Dis. 2005; 2(1): 38–49.
15- Runyen-Janecky L.J, Reeves S.A, Gonzales E.G, Payne S.M. Contribution of the Shigella flexneri Sit, Iuc, and Feo iron acquisition systems to iron acquisition in vitro and in cultured cells. Infect Immun. 2003; 71(4): 1919–1928.
16- Ewers C, Janßen T, Kießling S, Philipp H.C, Wieler L.H. Rapid detection of virulence associated genes in avian pathogenic Escherichia coli by multiplex polymerase chain reaction. Avian Dis. 2005; 49(2): 269-73.
17- Sabarinath A, Tiwari KP, Deallie C, Belot G, Vanpee G, Matthew V, et al. Antimicrobial resistance and phylogenetic groups of commensal Escherichia coli isolates from healthy pigs in Grenada. Webmed Central Veterinary Medicine. 2011; 2(5): WMC001942.
18- Moulin-Schouleur M, Répérant M, Laurent S, Brée A, Mignon-Grasteau S, Germon P, et al. Extraintestinal pathogenic Escherichia coli strains of avian and human origin: link between phylogenetic relationships and common virulence patterns. J Clin Microbiol. 2007; 45(10): 3366–3376.
19- Rodriguez-Siek K.E, Giddings C.W, Doetkott C, Johnson T.J, Fakhr M.K, Nolan L.K. Comparison of Escherichia coli isolates implicated in human urinary tract infection and avian colibacillosis. Microbiol. 2005; 151(6): 2097– 2110.
20- Ahmadi M, Dadashzadeh S, Ghaniei A. Prevalence of virulence genes in Escherichia coli isolates implicated in poultry colibacillosis and human urinary tract infection. J Vet Microbiol. 2019; 15(1): 109-118. [In Persian]
21- Kafshdouzan K.h, Zahraei Salehi T, Nayeri Fasaei B, Madadgar O, Yamasaki Sh, Hinenoya A, et al. Distribution of virulence associated genes in isolated Escherichia coli from avian colibacillosis. Iran J Vet Med. 2013; 7(1): 1-6.
22- Rodriguez-siek K.E, Giddings C.W, Doetkott C, Johnson T.J, Nolan L.K. Characterizing the APEC pathotype. Vet Res. 2005; 36(2): 241-256.
23- Faraz F, Mirzaei M. The frequency of sidrophores irp2 and iroN encoding genes in Escherichia coli clinical isolates. Journal of Isfahan Medical School. 2020; 37(560): 1448-1453. [In persian]
24- Imanpanah Z, Hashemi Tabar G.R, Askari Bedoui M, Ghazvini K. Evaluation of genes encoding iron absorption systems in Enteroaggregative Escherichia coli (EAEC) isolates isolated from diarrhea specimens. Medical Journal of Mashad University of Medical Sciences. 2023; 66(1): 132-137. [In Persian]
New Findings in Veterinary Microbiology
Volume 6, Issue 2
February 2024
Pages 83-89

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History

  • Receive Date: 22 September 2023
  • Revise Date: 21 November 2023
  • Accept Date: 06 January 2024

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