Assessment of antibiotic resistance of Acinetobacter baumannii isolated from indigenous and industrial eggs

Document Type : Original Article

Authors

1 Young Researchers and Elites Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

2 Department of Veterinary, College of Veterinary Medicine, Islamic Azad University of Shahrekord, Shahrekord, Iran

10.35066/J040.2018.603

Abstract

Acinetobacter species are widely distributed. There are also hypotheses regarding the transmission of this bacteria from food to humans. Eggs are always among the most consumed and richest human food sources. This study was conducted in order to evaluate the prevalence and antibiotic resistance of Acinetobacter baumannii in indigenous and industrial eggs. In this regard, a total of 100 indigenous and industrial egg samples were collected from Chahar Mahal and Bakhtiari province. Total of 200 samples, including the shell and content of eggs, were prepared. According to the standard methods, samples were cultured and examined for the presence of Acinetobacter baumannii. Colonies were identified by PCR method. Out of 200 shell and content samples, 2 shell samples (1%) were found to be infected with Acinetobacter baumannii. Antibacterial susceptibility testing of isolated bacteria to selected antibiotics was performed using disk diffusion method based on Clinical and Laboratory Standards Institute (CLSI) recommendations. Data were analyzed using SPSS 16 software and frequency distribution table. It was found that both tested samples were resistant to Tetracycline and Gentamicin antibiotics. One sample was resistant and the other one was intermediate to Trimethoprim and Ciprofloxacin. The minor resistance was observed to Carbenicillin. According to the available evidence, egg contamination with Acinetobacter baumannii occurs mainly through the environmental factors and secondary contamination. Lack of attention to the necessary points during the process of production, supply, storage and consumption of eggs can lead to the transfer of bacteria to the consumer. Therefore, it seems that it is possible to prevent the transmission of contamination to humans with timely monitoring and compliance with health guidelines in all the above steps.

Keywords

References

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New Findings in Veterinary Microbiology
Volume 1, Issue 1 - Serial Number 1
Spring & Summer 2018
October 2018
Pages 35-43

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History

  • Receive Date: 27 June 2018
  • Revise Date: 25 July 2018
  • Accept Date: 12 September 2018

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