Study of antimicrobial effects of ethanol extract of caper plant from Sis-tan on antibiotic resistant Salmonella typhimurium

Document Type : Original Article

Authors

10.35066/J040.2018.777

Abstract

Nowadays, antibacterial effects of medicinal plants are considered as an appropriate alternative of antibiotics. Therefore, the aim of the current study was in vitro determination of antimicrobial effects of caper (Capparis spinosa) against Salmonella typhmurium isolated from poultry. Ethanol extract of caper leaves was extracted using vacuum from the center (rotary) apparatus. Moreover, a total number of 12 S. typhmurium strains were isolated from poultry in Zabol. Minimum inhibitory concentration and minimum bactericidal concentration of different concentrations of plant extract were determined on bacteria using dilution in well method. Sensitivity of bacteria strains to antibiotics were evaluated using Kirby-Bauer disk diffusion standard test. Results of the current study showed that different strains of bacteria are resistant to Penicillin (100 %), Ampicillin (100 %), Tetracycline (16.6 %) and Amikacin (8.3 %). Furthermore, ethanol extracts of caper plant showed significant inhibitory effect on S. typhimurium. Results of the current study indicated that there is possibly of using ethanol extract of caper plant as a useful alternative beside usual antibiotics.

Keywords


1- Bartlett JG, Froggatt JW. Antibiotic resistance. Arch Otolaryngol Head Neck Surg. 1995; 121(4):392-6.
2- Cellai Rustici M, Mangiantini F, Chiappini E, Bartolome R, Pecile P, Prats G. Antibiotic resistance among Salmonella enterica isolates in Southern European children hospitalized for acute diarrhea. Eur J Pediatr. 2006; 165(8):577-8.
3- Ouderkirk LA. Evaluation of two microbiological methods for detecting residual antibiotics in milk. J Assoc off Anal Chem.1976; 59(5):1122-24.
4- Kjeldgaard J, Cohn MT, Casey PG, Hill C, Ingmer H. Residual antibiotics disrupt meat fermentation and increase risk of infection. MBio. 2012; 3(5):1-4.
5- Ezhov VI. Residual amounts of antibiotics in the eggs of laying hens. Veterin. 1970; 46(4):81-3.
6- Haznedaroglu BZ, Yates MV, Maduro MF, Walker SL. Effects of residual antibiotics in groundwater on Salmonella typhimurium: changes in antibiotic resistance, in vivo and in vitro pathogenicity. J Environ Monit. 2012; 14(1):41-7.
7- Islam MM, Yang CJ. Efficacy of mealworm and super mealworm larvae probiotics as an alternative to antibiotics challenged orally with Salmonella and E. coli infection in broiler chicks. Poult Sci. 2017; 96(1):27-34.
8- Hume ME. Historic perspective: prebiotics, probiotics, and other alternatives to antibiotics. Poult Sci. 2011; 90(11):2663-9.
9- Vahid H, Rakhshandeh H, Ghorbani A. Antidiabetic properties of Capparis spinosa L. and its components. Biomed Pharmacother. 2017; 92:293-302.
10- Alcantara M, Morales M, Carnes J. Food allergy to caper (Capparis spinosa). J Investig Allergol Clin Immunol. 2013; 23(1):67-9.
11- Yang T, Wang C, Liu H, Chou G, Cheng X, Wang Z. A new antioxidant compound from Capparis spinosa. Pharmaceutical biol. 2010; 48(5):589-94.
12- Zhou H, Jian R, Kang J, Huang X, Li Y, Zhuang C. Anti-inflammatory effects of caper (Capparis spinosa L.) fruit aqueous extract and the isolation of main phytochemicals. J Agric Food Chem. 2010; 58(24):12717-21.
13- Coates AR, Hu Y. Novel approaches to developing new antibiotics for bacterial infections. British J Pharmacol. 2007; 152(8):1147-54.
14- Zaidi MB, Calva JJ, Estrada-Garcia MT, Leon V, Vazquez G, Figueroa G. Integrated food chain surveillance system for Salmonella spp. in Mexico. Emerg Infect Dis. 2008; 14(3):429-35.
15- Ranjbar Malidareh N, Firouzi S, Ranjbar Malidareh N, Habibi H. In vitro and in vivo susceptibility of Salmonella spp. isolated from broiler chickens. Comp Clinic Path. 2012; 22:1065-8.
16- Skov MN, Madsen JJ, Rahbek C, Lodal J, Jespersen JB, Jorgensen JC. Transmission of Salmonella between wildlife and meat-production animals in Denmark. J Appl Microbiol. 2008; 105(5):1558-68.
17- Schikora A, Garcia AV, Hirt H. Plants as alternative hosts for Salmonella. Trends Plant Sci. 2012; 17(5):245-9.
18- Khan H. Medicinal Plants in Light of History: Recognized Therapeutic Modality. Evid Based Complement Alternat Med. 2014; 19(3):216-9.
19- Lipp FJ. The efficacy, history, and politics of medicinal plants. Altern Ther Health Med. 1996; 2(4):36-41.
20- Nabavi SM, Marchese A, Izadi M, Curti V, Daglia M, Nabavi SF. Plants belonging to the genus Thymus as antibacterial agents: from farm to pharmacy. Food Chem. 2015; 173:339-47.
21- Wafa BA, Makni M, Ammar S, Khannous L, Hassana AB, Bouaziz M. Antimicrobial effect of the Tunisian Nana variety Punica granatum L. extracts against Salmonella enterica (serovars Kentucky and Enteritidis) isolated from chicken meat and phenolic composition of its peel extract. International Journal of Food Microbiol. 2017; 241:123-31.
22- Tavakoli HR, Mashak Z, Moradi B, Sodagari HR. Antimicrobial activities of the combined use of Cuminum Cyminum L. Essential Oil, Nisin and Storage Temperature Against Salmonella typhimurium and Staphylococcus aureus in vitro. Jundi J of Microbiol. 2015; 8(4):e24838.
23- Mazarei F, Jooyandeh H, Noshad M, Hojjati M. Polysaccharide of caper (Capparis spinosa L.) Leaf: Extraction optimization, antioxidant potential and antimicrobial activity. Int J Biol Macromol. 2017; 95:224-31.
24- Boga C, Forlani L, Calienni R, Hindley T, Hochkoeppler A, Tozzi S. On the antibacterial activity of roots of Capparis spinosa L. Nat Prod Res. 2011; 25(4):417-21