Frequency of Tetracycline Resistance in Enterococcus Isolated from food

Document Type : Original Article

Authors

1 MSc in Microbiology, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

2 Associate professor, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

3 PhD Student in Virology, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

4 PhD Student in Bacteriology, Department of Medical Science, Qom Branch, Islamic Azad University, Qom, Iran

5 PhD Student in Microbiology, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

6 Assistant professor, Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

Abstract

Enterococcus as a part of the normal flora of human gastrointestinal tracts can cause infections in foods. Aim of this study was to determine tetracycline resistance in Enterococcus isolated from food. In this laboratory study, one hundred samples of food from different parts of Qom were collected. After determining the type, they were tested for their resistance against tetracycline genes by specific primer PCR. Out of 53 isolates, 45% isolates belonged to Enterococcus faecium 55% isolates were Enterococcus faecalis. Size band of this genes were tetS 647bp, tetO 442 bp, tetM 373 bp, tetL 306bp, ermb 311 bp. Among this samples tetO (12 %), tetS (6 %), tetM (17 %), tetL( 5%) and ermB (7%)isolate. Considering the results, increasing antibiotic resistant strains, especially tetracycline among Enterococcus, is a serious threat to the general public, Molecular method could help rapid diagnosis this resistance genes and help to treatment.

Keywords

References

1-Al-Ahdal MN, Abozaid SM, Al-Shammary HF, Bohol MF, Al-Thawadi SI, Al-Jaberi AA, et al. Characterization of Enterococcus faecium isolates and first report of vanB phenotype-van A genotype incongruence in the Middle East. European journal of clinical microbiology & infectious diseases: official publication of the European Society of Clinical Microbiology. 2012;31(11):3223-9
2- Arias CA, Murray BE. The rise of the Enterococcus: beyond vancomycin resistance. Nature Review Microbiology; 2012, 10(4):266- 78.
3-Cetinkaya Y, Falk P, Mayhall CG. Vancomycin-Resistant Enterococci. Clinical Microbiology Reviews. 2000;13(4):686-707.
4-Doi Y, Arakawa Y. 16S ribosomal RNA methylation: emerging resistance mechanism against aminoglycosides. Clinical Infectious Diseases. 2007;45(1):88-94
5- Facklam R, Collins M. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J. Clin. Microbiol;1989, 27(4): 731-4.
6-Farivar TN, Najafipour R, Johari P, Aslanimehr M, Peymani A, Hashemi HJ, et al. Development andevaluation of a Quadruplex Taq Man real-time PCR assay for simultaneous detection of clinical isolates of Enterococcus faecalis, Enterococcus faecium and their vanA and vanB genotypes. Iranian journal of microbiology. 2015;6(5):335-40.(In Persian)
7- Jahan M, Krause DO, Holley RA.Antimicrobial resistance of Enterococcus species from meat and fermented meat products isolated by a PCR-based rapid screening method. Int. J. Food Microbiol; 2013,163(2): 89-95.

8- Hanna Różańska, Aleksandra Lewtak-Piłat, Jacek Osek, Antimicrobial resistance of Enterococcus faecalis isolated from meat, Bulletin of the Veterinary Institute in Pulawy, 2014, 59, 229-233

9-Giridhara Upadhyaya P, Ravikumar K, Umapathy B. Review of virulence factors of enterococcus: an emerging nosocomial pathogen. Indian journal of medical microbiology. 2009;27(4):301
10-GuodarziA, Zolfaghari MR, Rezazadeh M, Amouzandeh-Nobaveh A, Arjmandzadegan M, Ghaznavi-Rad E. Phenotypic and genotypic determination of aminoglycoside resistance in methicillin resistant Staphylococcus aureus isolated from nosocomial infections. Urmia Medical Journal. 2014;24(11):883-93
11- Klare I, Heier H, Claus H, Witte W. Environmental strains of Enterococcus faecium with inducible high-level resistance to glycopeptides. FEMS Microbiol. Lett; 1993. 106(1): 23-9.
12-Li W, Li J, Wei Q, Hu Q, Lin X, Chen M, et al. Characterization of aminoglycoside resistance and virulence genes among Enterococcus spp. isolated from a hospital in China. International journal of environmental research and public health. 2015;12(3):3014-25.
13- M Pavia, C G. A. Nobile, L Salpietro, and I F. Angelillo, Vancomycin Resistance and Antibiotic Susceptibility of Enterococci in Raw Meat, Journal of Food Protection,2000, Vol. 63, No. 7, Pages 912–915
14- Mohsenzade M, Nasiri M, Kolalian Moghadam M.Detection of mecA gene in methicillin-resistant Staphylococcus aureus isolated from meat and hamburgers supplied in Mashhad. 7th National Congress of Biotechnology. 2011.
15- Mohammadi F, Tabaraie B, Sadeghifard N. Evaluation of drug resistance frequency among Entrococcus faecium and E. faecalis strains and detection of vanA/B genes in vancomycin resistance isolated by PCR method in Ilam and Kermanshah hospitals. Sci J Ilam Unive Med Sci; 2010.19: 1-8.
16-. Mokta KK, Verma S, Chauhan D, Ganju SA, Singh D, Kanga A, et al. Inducible Clindamycin Resistance among Clinical Isolates of Staphylococcus aureus from Sub Himalayan Region of India. Journal of clinical and diagnostic research: JCDR. 2015;9(8): DC20
17- Murray BE. The life and times of the Enterococcus. Clin. Microbiol Rev; 1990.3(1): 46-65.
18-.Ramirez MS, Tolmasky ME. Aminoglycoside modifying enzymes. Drug Resistance Updates. 2010;13(6):151-71.
19-. Rice LB. Antimicrobial resistance in gram-positive bacteria. The American journal of medicine. 2006;119(6 Suppl 1): S11-9; discussion S62-70
20-Sood S, Malhotra M, Das B, Kapil A. Enterococcal infections & antimicrobial resistance.
Indian Journal Medical Research. 2008;128(2):111-21.
21-Weinstein RA. Nosocomial infection update. Emerging Infectious Diseases. 1998;4(3):416-20.
22- M Zargar, A Javadi and A Morovvati, Detection of Clostridium Difficile Associated Diarrhea in Disease Based on Polymerase Chain Reaction and Bacterial Culture and Toxin A, B Frequency , Journal of Fasa University of Medical Sciences,2019, Vol. 9 , No. 3

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History

  • Receive Date: 11 September 2022
  • Accept Date: 21 September 2022

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