بررسی فعالیت ضد میکروبی عطرمایه‌ی زیره سبز، شوید، رازیانه، آویشن شیرازی و نعناع فلفلی بر روی اشریشیاکلی جدا شده از مدفوع طیور

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه پژوهشی زراعت و اصلاح نباتات، پژوهشکده کشاورزی، دانشگاه زابل، زابل، ایران

2 دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

چکیده

هدف از این مطالعه بررسی اثر عطرمایه‌های آویشن شیرازی، نعناع فلفلی، زیره سبز، شوید و رازیانه بر روی اشریشیاکلی جدا شده از مدفوع طیور است. عطرمایه‌ی گیاهان مورد استفاده با دستگاه کلونجر به دست آمد. جدایه‌های اشریشیاکلی از نمونه مدفوع طیور، جداسازی و در نهایت حداقل غلظت مهار کنندگی و حداقل غلظت کشندگی با روش میکرودایلوشن تعیین گردید. تمامی جدایه‌های اشریشیاکلی در غلظت، 56/1 میلی‌گرم بر میلی‌لیتر توسط هر دو گیاه آویشن شیرازی و نعناع فلفلی مهار شده‌اند. کمترین غلظت کشندگی (12/3 میلی‌گرم بر میلی‌لیتر) برای گیاه آویشن شیرازی و نعناع فلفلی به ترتیب باعث مهار یک و دو جدایه شده است. تمامی جدایه‌های اشریشیاکلی در غلظت، 63/0 میلی‌گرم بر میلی‌لیتر توسط هر سه گیاه زیره سبز، شوید و رازیانه مهار شده‌اند. کمترین غلظت کشندگی (25/1 میلی‌گرم بر میلی‌لیتر) برای گیاه زیره سبز باعث مهار دو جدایه شده است اما این غلظت برای شوید و رازیانه، 5 میلی‌گرم بر میلی‌لیتر بوده است. نتایج نشان داد که به ترتیب عطرمایه‌های زیره سبز، آویشن شیرازی، نعناع فلفلی و سپس رازیانه و شوید با تأکید بر مؤثرتر بودن عطرمایه زیره سبز، می‌توانند به ‌تنهایی یا همراه با سایر عوامل ضد میکروبی برای درمان عفونت‌های ناشی از باکتری اشریشیاکلی مفید باشند.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of antimicrobial activity of Zataria multiflora Bioss, Mentha piperita L, Cuminum cyminum L., Foeniculum vulgare Mill and Anethum graveolens L. essential oil on Escherichia coli isolated from poultry feces

نویسندگان [English]

  • Bahman Fazeli-Nasab 1
  • Pantea Ramazannezhad 2
  • yasoub shiri 1
1 Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran
2 Shahrekord University of Medical Sciences, Shahrekord, Iran
چکیده [English]

The aim of this study was to investigate the effect of thyme, peppermint, cumin, dill, and fennel essential oils on Escherichia coli isolated from poultry feces. The essential oil of the plants used was obtained with a Clevenger Apparatus. Escherichia coli strains were isolated from poultry feces, isolated, and finally the minimum inhibitory concentration and the minimum lethal concentration were determined by Fine-grained method. All strains of Escherichia coli at concentrations, 1.56 mg/ml were inhibited by both thyme and peppermint. The MBC (3.12 mg / ml) for Zataria multiflora and peppermint has inhibited one- and two-way, respectively. All Escherichia coli strains at concentrations of 0.63 mg/ml were inhibited by all three cumin, dill, and fennel plants. The MBC (1.25 mg / ml) for cumin has led to double-sided control, but the concentration for dill and fennel was 5 mg/ml. The results showed that cumin, thyme, peppermint and then fennel and dill essential oils, respectively, with emphasis on the effectiveness of cumin essential oil, can be useful alone or in combination with other antimicrobial agents to treat infections caused by Escherichia coli bacteria.

کلیدواژه‌ها [English]

  • Thyme
  • Peppermint
  • Escherichia coli
  • cumin
  • dill
  • fennel
 
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