بررسی فعالیت ضد میکروبی گیاه دارویی چریش تهیه شده با حلال‌های مختلف بر روی باکتری‌های بیماری‌زا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

2 گروه جراحی مغز و اعصاب، بیمارستان امیرالمومنین، دانشگاه علوم پزشکی زابل، زابل، ایران

3 دانشکده پزشکی، دانشگاه علوم پزشکی زاهدان، زاهدان، ایران

چکیده

چریش (Azadirachta indica) به‌عنوان یک گیاه دارویی به دلیل خواص ضد باکتریایی، ضد مالاریا، ضد ویروسی و ضد قارچی شناخته ‌شده است. هدف از این مطالعه بررسی فعالیت ضد میکروبی گیاه دارویی چریش تهیه ‌شده با حلال‌های مختلف بر روی باکتری‌های بیماری‌زا است. قسمت‌های مختلف گیاه دارویی چریش از منطقه سیستان و بلوچستان جمع‌آوری گردیده، در سایه خشک و با استفاده از حلال‌های مختلف عصاره‌گیری انجام شد و قطر هاله مهاری در رقت 100 میکروگرم/میلی‌لیتر در برابر سویه‌های باکتری بررسی گردید. نتایج حاصل از این مطالعه نشان داد که عصاره استونی میوه چریش بیشترین تأثیر بر انتروکوکوس فیکالیس، باسیلوس سرئوس و شیگلا دیسنتری داشته است و عصاره متانولی و اتانولی میوه بیشترین مهارکنندگی بر روی اشرشیاکلی و سودوموناس آئروژینوزا از خود نشان داده است همچنین عصاره استونی برگ بیشترین اثر مهارکنندگی بر روی لیستریا مونوسیتوژنز از خود نشان داده است. نتایج نشان داد که برگ و میوه گیاه دارویی چریش با حلال‌های مختلف، اثر مهاری متفاوتی علیه میکروب‌ها داشته‌اند.

کلیدواژه‌ها


عنوان مقاله [English]

Investigating the antimicrobial activity of Azadirachta indica medicinal plant prepared with different solvents on pathogenic bacteria

نویسندگان [English]

  • Habib Dahmardeh 1
  • Reza Rohani 2
  • Zahra Beigomi 3
1 Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
2 Department of Neurosurgery, Amiralmomenin Hospital, Zabol University of medical sciences, Zabol, Iran
3 Zahedan University of Medical Sciences, Zahedan, Iran.
چکیده [English]

Azadirachta indica is known as a medicinal plant due to its antibacterial, antimalarial, antiviral and antifungal properties. The purpose of this study is to investigate the antimicrobial activity of Azadirachta indica medicinal plant prepared with different solvents on pathogenic bacteria. Different parts of Azadirachta indica medicinal plant were collected from Baluchistan-Sistan and Baluchistan region, extraction was done in dry shade and using different solvents, and the diameter of the inhibitory halo in a dilution of 100 mg/ml against Bacterial strains were investigated. The results of this study showed that the greatest effect of Azadirachta indica medicinal plant on Enterococcus phicalis, Bacillus cereus and Shigella dysentery was obtained from acetone extract and fruit tissue. Against Escherichia coli obtained from methanol extract and fruit tissue. Against Pseudomonas aeruginosa obtained from ethanol extract and fruit tissue. It was against Listeria monocytogenes obtained from acetone extract and leaf tissue. The results of this study showed that the leaves and fruits of Azadirachta indica medicinal plant with different solvents had different inhibitory effects against microbes.

کلیدواژه‌ها [English]

  • extraction solvents
  • Azadirachta indica plant
  • pathogenic bacteria
  • halo diameter of growth inhibition
  1. 1- Ali E, Islam S, Hossen I, Khatun M, Islam A. Extract of neem (Azadirachta indica) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry. Vet Med Sci. 2021; 7(5): 1921–1927.

    2- Abalaka M, Oyewole OA, Kolawole AR. Antibacterial activities of azadirachta indica against some bacterial pathogens. Adv Life Sci. 2012; 2(2): 5-8.

    3- Bharitkar YP, Bathini S, Ojha D, Ghosh S, Mukherjee H, Kuotsu K, et al. Antibacterial and antiviral evaluation of sulfonoquinovosyldiacylglyceride: A glycolipid isolated from Azadirachta indica leaves. Letters in Applied Microbiology. 2014; 58: 184–189.

    4- Chibuzo UC. Antimicrobial Activity of Azadirachta indica (Neem) Leaf Extract on Some Bacteria. Int J Curr Microbiol Appl Sci. 2019; 8: 431-437.

    5- Deng Y, Shi D, Yin Z, Guo J, Jia R, Xu J, et al. Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatography against Sarcoptes scabiei var. cuniculi larvae in vitro. Experimental Parasitology. 2012; 130(4): 475-477.

    6- Fernández-Grandon GM, Harte SJ, Ewany J, Bray D, Stevenson PC. Additive Effect of Botanical Insecticide and Entomopathogenic Fungi on Pest Mortality and the Behavioral Response of Its Natural Enemy. Plants. 2020; 9(2): 173.

    7- Fazeli-Nasab B, Sirousmehr A, Mirzaei N, Solimani M. Evaluation of total phenolic, flavenoeid content and antioxidant activity of Leaf and Fruit in 14 different genotypes of Ziziphus mauritiana L. in south of Iran. Eco-phytochemical Journal of Medicinal Plants. 2017; 4(4): 1-14.

    8- Fooladvand Z, Fazeli-nasab B. Antibacterial activities of Stachys lavandulifolia Vahl. extract against eight bacteria. Journal of Herbal Drugs (An International Journal on Medicinal Herbs). 2014; 5(1): 13-18.

    9- Francine U, Jeannette U, Pierre RJ. Assessment of antibacterial activity of neem plant (Azadirachta indica) on Staphylococcus aureus and Escherichia coli. J Med Plants Stud. 2015; 3(4): 85-91.

    10- Gogi MD, Syed AH, Atta B, Sufyan M, Arif MJ, Arshad M, et al. Efficacy of biorational insecticides against Bemisia tabaci (Genn.) and their selectivity for its parasitoid Encarsia formosa Gahan on Bt cotton. Scientific reports. 2021; 11(1): 1-12.

    11- Grover A, Bhandari B. S, Rai N. Antimicrobial activity of medicinal plants-Azadirachta indica A. Juss, Allium cepa L. and Aloe vera L. Journal of Pharm Tech Research. 2011; 3(2): 1059-1065.

    12- Hossain MA, Al-Toubi WA, Weli AM, Al-Riyami QA, Al-Sabahi JN. Identification and characterization of chemical compounds in different crude extracts from leaves of Omani neem. Journal of Taibah University for Science. 2013; 7(4): 181-188.

    13- Jagannadh JH, Radhika M. Antimicrobial emulsion (coating) based on biopolymer containing neem (meliaazadirachata) and turmeric (curcuma longa) extract for wound healing. Biomed Mater Eng. 2006; 16(5): 329-36.

    14- Jain D, Jayaram L, Prabhu MV, Bhat K. Antibacterial effect of neem (Azadirachta indica) oil on multidrug resistant bacteria isolated from human infections. International Journal of Biological and Medical Research. 2013; 4: 3544–3546.

    15- Jerobin J, Makwana P, Suresh Kumar R, Sundaramoorthy R, Mukherjee A, Chandrasekaran N. Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro. International journal of nanomedicine. 2015; 10(2): 77-86.

    16- Koona S, Budida S. Antimicrobial potential of the extracts of the leave of azadirachta indica linn. Nat Sci Biol. 2011; 3(1): 65-9.

    17- Khanavi M, Hasanloo T, Hajimehdipoor H. Quantitative Determination of Azadirachtin in Melia indica and M. azedarach. Journal of Medicinal Plants. 2019; 18(69): 185-193.

    18- Kurita N, Miyaji M, Kurane R, Takahara Y. Antifungal activity of components of essential oils. Agricultural and Biological Chemistry. 1981; 45(4): 945-952.

    19- Maleki L, Sadeghian-Rizi T, Ghannadian M, Sanati M H, Shafizadegan S, Sadeghi-Aliabadi H. Antibacterial activity of Azadirachta indica leaf extracts against some pathogenic standards and clinical bacterial isolates. Avicenna Journal of Clinical Microbiology and Infection. 2017; 5(1): 12987-12987.

    1. Mahmoud D, Hassanein N, Youssef K, Abou Zeid M. Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens. Brazilian Journal of Microbiology. 2011; 42: 1007-1016.

    21- Mamman PH, Mshelia WP, Susbatrus SC, Sambo KW. Antibacterial effects of crude extract of Azadirachta indica against Escherichia coli, Salmonella spp and Staphylococcus aureus. International Journal of Medicine and Medical Sciences. 2013; 5(1): 14-18.

    22- Mondall N, Mojumdar A, Chatterje S, Banerjee A, Datta J, Gupta S. Antifungal activities and chemical characterization of Neem leaf extracts on the growth of some selected fungal species in vitro culture medium. J Appl Sci Environ Manage. 2009; 13(1): 49-53.

    23- Allan EJ, Eeswara P, Johnson S, Mordue AJ (Luntz), Morgan ED, Stuchbury T. The production of azadirachtin in-vitro tissue culture of neem, Azadirachta indica Pestic. Sci.1994; 42: 147-152.

    24- Nathan SS, Kalaivani K, Murugan K. Effects of neem limonoids on the malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Acta tropica. 2005; 96(1): 47-55.

    25- Patil SP, Chaudhari RY, Nemade MS. Azadirachta indica leaves mediated green synthesis of metal oxide nanoparticles: A review. Talanta Open. 2022; 5: 100083.

    26- Patil SM, Shirahatti PS, V B C K, Ramu R, M N N P. Azadirachta indica A. Juss (neem) as a contraceptive: An evidence-based review on its pharmacological efficiency. Phytomedicine. 2021; 88: 153596.

    27- Quelemes PV, Perfeito ML, Guimarães MA, dos Santos RC, Lima DF, Nascimento C. Effect of Neem (Azadirachta indica A. Juss) Leaf Extract on Resistant Staphylococcus aureus Biofilm Formation and Schistosoma Mansoni Worms. J. Ethnopharmacol. 2015; (175): 287–294.

    28- Rasool M, Malik A, Arooj M, Alam MZ, Alam Q, Awan M, et al. Evaluation of antimicrobial activity of ethanolic extracts of Azadirachta indica and Psidium guajava against clinically important bacteria at varying pH and temperature. Biomed Res. 2017; 28: 134-139.

    29- Ramesh P, Okigbo R. Effects of plants and medicinal plant combinations as anti-infectives. Afr J Pharm Pharmacol. 2008; 2(7): 130-135

    30- Santhosh V, Navaratnam V. Neem (Azadirachta indica): Prehistory to contemporary medicinal uses to humankind. Asian Pacific J Trop Biomed. 2013; 3(7): 505-514.

    31- Sarmiento WC, Maramba CC, Gonzales MLM. An in-vitro study on the antibacterial effect of neem (Azadirachta indica) leaf extract on methicillin-sensitive and Methicillin-resistant Staphylococcus aureus. PIDSP J. 2011; 12(1): 40-45.

    32- S L P, A U, S J G F. Investigation on the Biofilm Eradication Potential of Selected Medicinal Plants against Methicillin-Resistant Staphylococcus aureus. Biotechnol. Rep. 2020; 28, e00523.

    33- Vestby LK, Grønseth T, Simm R, Nesse LL. Bacterial Biofilm and its Role in the Pathogenesis of Disease. Antibiot. 2020; 9(2).

    34- Valizadeh M, Beigomi M, Fazeli-Nasab B. Antibacterial and Anti biofilm effects of ethanol and aceton leaf extract of Momordica charantia and Tecomella undulata against Acinetobacter baumannii. Int J Adv Biol Biomed Res. 2020; 8(4): 403-418.