تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

بررسی سرو اپیدمیولوژیک بیماری تب مالت در گاوها و گوسفندان شهرستان شیراز

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه دامپزشکی، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر، ایران
2 گروه دامپزشکی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران
چکیده
بروسلوز یکی از شایع‌ترین بیماری‌های عفونی در ایران بوده که مشترک بین انسان و دام می‌باشد و توسط گونه‌های جنس بروسلا ایجاد می‌گردد. یکی از راه‌های انتقال بروسلوز به انسان مصرف شیر و فرآورده‌های سنتی آلوده به باکتری بروسلا می‌باشد. تحقیق حاضر به منظور بررسی سرو اپیدمیولوژیک تب مالت در گاوها و گوسفندان شهرستان شیراز صورت گرفت. از این‌رو 120 نمونه سرمی از گاو‌ها و 80 نمونه سرمی از گوسفندان گرفته شد. ابتدا آزمایش رزبنگال به‌عنوان آزمون سریع اولیه تشخیصی، بر روی تمام نمونه‌های سرمی انجام شد و مشاهده گردید که از 200 نمونه آزمایش رزبنگال، 5 درصد مثبت بودند. سپس نمونه‌های مثبت در آزمایش رزبنگال تحت آزمایش رایت لوله‌ای قرار گرفتند و مشاهده شد که 4 درصد نمونه‌ها مثبت هستند. سپس جهت تشخیص نوع پادتن و تعیین حالت فعال و یا غیر فعال بیماری بر روی سرم‌های مذکور آزمایش دو مرکاپتواتانول انجام شد که 5/3 درصد مثبت بود. با توجه به نتایج مذکور، میزان شیوع بیماری بروسلوز در گاوها و گوسفندان 5/3 درصد گزارش شد، که ماده بودن (4/7OR=)، مسن بودن (87/7OR=) و نگهداری سنتی (27/7OR=) خطر ابتلا به بروسلوز را در گاوها و گوسفندان افزایش می‌دهد. در تحقیق حاضر، آلودگی در دام‌های مسن بیشتر از دام‌های جوان مشاهده شد. در نهایت مشاهده شد که آلودگی در دام‌هایی که به روش سنتی نگهداری می‌شوند بیشتر از دام‌هایی است که در واحدهای صنعتی و تولیدی هستند.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Seroepidemiological Study of Brucellosis in Cows and Sheep in Shiraz

نویسندگان English

Davoud Hoshyar 1
Seyadeh Ommolbanin Ghasemian 2
1 Department of Veterinary, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran
2 Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran
چکیده English

Brucellosis is one of the most common infectious diseases in Iran, which is common between humans and animals and is caused by Brucella species. One of the ways of brucellosis transmission to humans is the consumption of milk and traditional products contaminated with Brucella bacteria. The present study was conducted to investigate the epidemiological situation and the incidence of malt fever in cattle and sheep in Shiraz city. 120 serum samples were taken from cows and 80 serum samples from sheep. First, the rose bengal test was performed on all serum samples as a rapid initial diagnostic test, and it was observed that 5% of the 200 rose bengal test samples were positive. Then the positive samples in the Rose Bengal test were subjected to the Wright tube test and it was observed that 4% of the samples were positive. Then, in order to detect the type of antibody and to determine the active or inactive state of the disease, two mercaptoethanol tests were performed on the said sera, which was 3.5% positive. According to the mentioned results, the prevalence of Brucellosis in cattle and sheep was reported to be 3.5%, and being female (OR=7.4), being old (OR=7.87) and traditional keeping (OR=7.27) increase the risk of brucellosis in cows and sheep. In the present research, contamination was observed in old animals more than in young animals. It was observed that the contamination in the animals that are kept in the traditional way is more than the animals that are in industrial and production units.

کلیدواژه‌ها English

Brucellosis
cattle
sheep
Shiraz
1- Zhou K, Wu B, Pan H, Paudyal N, Jiang J, Zhang L, et al. ONE health approach to address zoonotic brucellosis: a spatiotemporal associations study between animals and humans. Frontiers in Veterinary Science. 2020;7:521.
2- Zeinali M, Doosti S, Amiri B, Gouya MM, Godwin GN. Trends in the Epidemiology of Brucellosis Cases in Iran during the Last Decade. Iranian Journal of Public Health. 2022;51(12):2791.
3- Atluri VL, Xavier MN, de Jong MF, den Hartigh AB, Tsolis RM. Interactions of the human pathogenic Brucella species with their hosts. Annual review of microbiology. 2011;65:523-41.
4- Dadar M, Shahali Y, Fakhri Y. Brucellosis in Iranian livestock: A meta-epidemiological study. Microbial Pathogenesis. 2021;155:104921.
5- Keramat F, Karami M, Alikhani MY, Bashirian S, Moghimbeigi A, Hashemi SH, et al. Seroprevalence of Brucellosis among general population in Famenin city, western Iran in 2016: The Famenin Brucellosis cohort study. Archives of Iranian medicine. 2020;23(11):749-56.
6- Rodríguez-Ezpeleta N, Embley TM. The SAR11 group of alpha-proteobacteria is not related to the origin of mitochondria. PloS one. 2012;7(1):e30520.
7- Martijn J, Vosseberg J, Guy L, Offre P, Ettema TJ. Deep mitochondrial origin outside the sampled alphaproteobacteria. Nature. 2018;557(7703):101-5.
8- Parte AC, Sardà Carbasse J, Meier-Kolthoff JP, Reimer LC, Göker M. List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. International journal of systematic and evolutionary microbiology. 2020;70(11):5607-12.
9- Hördt A, López MG, Meier-Kolthoff JP, Schleuning M, Weinhold L-M, Tindall BJ, et al. Analysis of 1,000+ type-strain genomes substantially improves taxonomic classification of Alphaproteobacteria. Frontiers in microbiology. 2020;11:468.
10- Adabi M, Khazaiee S, Sadeghi-Nasab A, Alamian S, Arabestani MR, Valiei Z, et al. Brucellosis in livestock: First study on seroepidemiology, risk factors, and preventive strategies to manage the disease in Famenin, Iran. Veterinary World. 2022;15(8):2102.
11- Golshani M, Bouzari S. A review of brucellosis in Iran: epidemiology, risk factors, diagnosis, control, and prevention. Iranian biomedical journal. 2017;21(6):349.
12- Mirnejad R, Jazi FM, Mostafaei S, Sedighi M. Epidemiology of brucellosis in Iran: A comprehensive systematic review and meta-analysis study. Microbial pathogenesis. 2017;109:239-47.
13- Bahmani N, Bahmani A. A review of brucellosis in the Middle East and control of animal brucellosis in an Iranian experience. Reviews and Research in Medical Microbiology. 2022;33(1):e63-e9.
14- ZareBidaki M, Allahyari E, Zeinali T, Asgharzadeh M. Occurrence and risk factors of brucellosis among domestic animals: An artificial neural network approach. Tropical Animal Health and Production. 2022;54:1-13.
15- Williamson B. Victory in the Battle against Brucella: From bench to battlefield. Vital Record: News from Texas A&M HSC; 2013.
16- Bennett JE, Dolin R, Blaser MJ. Mandell, Douglas, and Bennett's principles and practice of infectious diseases: Elsevier Inc.; 2014.
17- Brown PJ, de Pedro MA, Kysela DT, Van der Henst C, Kim J, De Bolle X, et al. Polar growth in the Alphaproteobacterial order Rhizobiales. Proceedings of the National Academy of Sciences. 2012;109(5):1697-701.
18- Almuzaini AM. An Epidemiological Study of Brucellosis in Different Animal Species from the Al-Qassim Region, Saudi Arabia. Vaccines. 2023;11(3):694.
19- Omer M, Skjerve E, Holstad G, Woldehiwet Z, Macmillan A. Prevalence of antibodies to Brucella spp. in cattle, sheep, goats, horses and camels in the State of Eritrea; influence of husbandry systems. Epidemiology & Infection. 2000;125(2):447-53.
20- Samaha H, Al-Rowaily M, Khoudair RM, Ashour HM. Multicenter study of brucellosis in Egypt. Emerging infectious diseases. 2008;14(12):1916.
21- Al-Majali AM, Talafha AQ, Ababneh MM, Ababneh MM. Seroprevalence and risk factors for bovine brucellosis in Jordan. Journal of Veterinary Science. 2009;10(1):61-5.
22- Akoko JM, Pelle R, Lukambagire AS, Machuka EM, Nthiwa D, Mathew C, et al. Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya. Scientific Reports. 2021;11(1):8881.
23- Racloz V, Schelling E, Chitnis N, Roth F, Zinsstag J. Persistence of brucellosis in pastoral systems. OIE Revue Scientifique et Technique. 2013;32(1):61-70.
24- Ng’ang’a CM. Cultural Drivers of Brucellosis and Treatment Pathways for Febrile Illnesses Among Agro Pastoralists in Kilombero District, Tanzania: University of Nairobi; 2022.
25- Legesse A, Mekuriaw A, Gelaye E, Abayneh T, Getachew B, Weldemedhin W, et al. Comparative evaluation of RBPT, I-ELISA, and CFT for the diagnosis of brucellosis and PCR detection of Brucella species from Ethiopian sheep, goats, and cattle sera. BMC microbiology. 2023; 23(1): 216.
26- Lokamar PN, Kutwah MA, Atieli H, Gumo S, Ouma C. Socio-economic impacts of brucellosis on livestock production and reproduction performance in Koibatek and Marigat regions, Baringo County, Kenya. BMC veterinary research. 2020; 16(1): 1-13.
27- Meena DS, Sharma L, Bishnoi J, Soni M, Jeph NK, Galav V, et al. Serological and molecular prevalence of Brucella spp. among livestock species in Rajasthan, India. Frontiers in Veterinary Science. 2023; 10: 1157211.
28- Bayasgalan C, Chultemdorj T, Roth F, Zinsstag J, Hattendorf J, Badmaa B, et al. Risk factors of brucellosis seropositivity in Bactrian camels of Mongolia. BMC veterinary research. 2018;14:1-11.
29- Mekonnen K. Study on camel and human brucellosis in Fentale District, East Shoa Zone, Oromia regional state, Ethiopia. J Biol Agric Healthc. 2016;6:117-45.
30- Fatima S, Khan I, Nasir A, Younus M, Saqib M, Melzer F, et al. Serological, molecular detection and potential risk factors associated with camel brucellosis in Pakistan. Tropical Animal Health and Production. 2016;48:1711-8.
31- Tadesse G. Brucellosis seropositivity in animals and humans in Ethiopia: A meta-analysis. PLoS neglected tropical diseases. 2016; 10(10): e0005006.
32- Dadar M, Omar SS, Shahali Y, Fakhri Y, Godfroid J, Khaneghah AM. The prevalence of camel brucellosis and associated risk factors: a global meta-epidemiological study. Quality Assurance and Safety of Crops & Foods. 2022; 14(3): 55-93.
دوره 7، شماره 1
بهار و تابستان
شهریور 1403
صفحه 54-64

  • تاریخ دریافت 22 مهر 1402
  • تاریخ بازنگری 27 اسفند 1402
  • تاریخ پذیرش 22 اردیبهشت 1403