تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

بررسی بهبود زخم تلقیح شده با باکتری استافیلوکوکوس اورئوس توسط عصاره گیاه گل راعی و روغن شترمرغ

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانش‌آموخته، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران
2 استادیار، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران
3 دانشیار، گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران
4 استادیار، گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران
چکیده
استافیلوکوکوس اورئوس یکی از مهم‌ترین عوامل باکتریایی عفونت زخم‌های پوستی است. در طب مکمل، استفاده از گیاهان دارویی به‌دلیل خواص ضد باکتری، ضد التهاب و آنتی‌اکسیدانی مورد توجه قرار می‌گیرد. در این مطالعه، اثر ترکیب عصاره گل راعی و روغن شترمرغ بر التیام زخم باز تلقیح‌شده با استافیلوکوکوس اورئوس در رت مورد بررسی قرار گرفت. تعداد ۴۰ رت بالغ در چهار گروه شامل کنترل منفی، کنترل مثبت (وازلین+ اوسرین)، و دو گروه درمانی با پمادهای حاوی 2 و 5 درصد ترکیب مذکور مورد مطالعه قرار گرفت. زخم‌ها در روزهای ۰، ۳، ۷ و ۱۴ از لحاظ درصد بهبود و تغییرات هیستوپاتولوژیک بررسی شدند. یافته‌ها نشان داد که درصد بهبود زخم در روز 7 به‌طور معنی‌داری در دو گروه درمانی بیشتر از گروه کنترل مثبت بود، همچنین میانگین رتبه هیستوپاتولوژی زخم‌ها در روزهای 7 و 14 در گروه‌های درمانی به‌طور معنی‌داری بالاتر از گروه کنترل منفی بود. نتایج نشان می‌دهد ترکیب عصاره گل راعی و روغن شترمرغ سبب کاهش التهاب و تسریع در بهبودی زخم عفونی می‌شود و اثر پماد 5 درصد بیشتر از پماد 2 درصد است.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Evaluation of Wound Healing Inoculated with Staphylococcus aureus by St. John's Wort Extract and Ostrich Oil

نویسندگان English

Roxana Sarabandi 1
Mohammad Reza Aghchelou 2
Dariush Saadati 3
Abbas Jamshidian 4
1 Gradated student, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
2 Assistant professor, Department of Clinical Science, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
3 Associated professor, Department of Food Hygiene, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
4 Assistant professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
چکیده English

Abstract: Staphylococcus aureus is one of the most significant bacterial agents in skin wound infections. In complementary medicine, medicinal plants are of interest due to their antibacterial, anti-inflammatory, and antioxidant properties. This study investigates the effect of a combination of St. John's Wort extract and ostrich oil on the healing of open wounds inoculated with S. aureus in rats. Forty adult rats were divided into four groups: a negative control, a positive control (Vaseline + Eucerin), and two treatment groups with ointments containing 2% and 5% of the mentioned combination. The wounds were examined on days 0, 3, 7, and 14 for healing percentage and histopathological changes. Findings showed that the healing percentage on day 7 was significantly higher in the two treatment groups compared to the positive control group. Additionally, the mean histopathology scores of the wounds on days 7 and 14 significantly better in the treatment groups than in the negative control group. The results indicate that the combination of St. John's Wort extract and ostrich oil reduces inflammation and accelerates the healing of infected wounds, with the 5% ointment showing greater efficacy than the 2% ointment.

کلیدواژه‌ها English

Staphylococcus aureus
St. John's Wort extract
ostrich oil
histopathology
wound healing
1- Duke JA. Handbook of Medicinal Herbs. CRC Press Inc. Boca Raton. 2002; P: 374- 375.
2- Simor AE, Phillips E, McGeer A, et al. Mupirocin resistance in Staphylococcus aureus from a tertiary care hospital. J Clin Microbiol. 2006; 44(9): 2877-2880.
3- Ghaderi R, Zardast M, Hosseini M, Delgir B, Hassanpour M. Comparison of Antibacterial Effect of Cichorium Intybus L. with Vancomycin, Ceftriaxone, Ciprofloxacin and Penicillin (In Vitro). Clin Exp Pharmacol. 2012; 2(2): 1000113. [In persian]
4- Morton LM, Phillips TJ. Wound Healing Update. Semin Cutan Med Surg. 2012; 31(1): 33-7.
5- Shiu WKP, Malkinson JP, Rahman MM, Curry J, Stapleton P, Gunaratnam M, et al. A new plant-derived antibacterial is an inhibitor of efflux pumps in Staphylococcus aureus. Int J Antimicrob Agents. 2013; 42(6): 513-8.
6- Reichling J, Weseler A, Saller R. A current review of the antimicrobial activity of Hypericum perforatum L. Pharmacopsychiatry. 2001; 34(1): S116 - S118.
7- Wölfle U, Seelinger G, Schempp CM. Topical Application of St. John's Wort (Hypericum perforatum). Planta Medica. 2014; 80(2- 3): 109-20.
8- Schempp CM, Windeck T, Hezel S, Simon JC. Topical treatment of atopic dermatitis with St. John's wort cream–a randomized, placebo controlled, double blind half-side comparison. Phytomedicine. 2003; 10(4): 31-37.
9- Gavanji S, Larki B, Taraghian AH. A review of Application of Ostrich oil in Pharmacy and Diseases treatment. JNAS. 2013; 2013-2-11: 650-654.
10- El-Kamali HH. Folk medicinal use of some animal products in Central Sudan. J Ethnopharmacol. 2000; 72: 279-282.
11- Grompone MA, Irigaray B, Gil M. Composition and thermal properties   of Rhea oil and its fractions. Eur J Lipid Sci Technol. 2005; 107: 762-766.
12- Snowden JM, Whitehouse MW. Anti-inflammatory activity of emu oils in rats. Inflammopharmacology. 1997; 5: 127-132.
13- Abimosleh SM, Tran CD, Howarth GS. Emu Oil: A novel therapeutic for disorders of the gastrointestinal tract? J Gastroenterol hepatol. 2012; 27: 857-861.
14- Politis MJ, Dmytrowich A. Promotion of second intention wound healing by emu oil lotion: comparative results with furasin, polysporin, and cortisone. Plast Recon Surg. 1998; 102: 2404– 2407.
15- Winn WC, Allen SD, Allen S. Koneman's color atlas and textbook of diagnostic microbiology.London: Lippincott Williams & Wilkins 1997; 200: 211-94.
16- Singer AJ, Thode HC, McClain SA. Development of a histomorphologic scale to quantify cutaneous scars after burns. Acad Emerg Med. 2000; 7: 1083- 1088.
17- Sanadgol S, Aghchelou MR, Darush S, Jamshidian A. Effects of Hypericum perforatum and Aloe vera extracts in rat open wounds inoculated with Staphylococcus aureus: clinical and histopathology aspects. NFVM. 2021; 4 (1): 1-22. [In persian]
18- Sherif MM, Elshikh HH, Abdel-Aziz MM, Elaasser MM, Yosri M. In Vitro Antibacterial and Phytochemical Screening of Hypericum perforatum Extract as Potential Antimicrobial Agents against Multi-Drug-Resistant (MDR) Strains of Clinical Origin. Biomed Res Int. 2023; 2023: 6934398.
19- Farahpour MR, Vahid M, Oryan A. Effectiveness of topical application of ostrich oil on the healing of Staphylococcus aureus- and Pseudomonas aeruginosa-infected wounds. Connect Tissue Res. 2018; 59(3): 212-222. [In persian]
20- Guleken Z, Depciuch J, Ege H, İlbay G , Kalkandelen C, Ozbeyli D, et al., Spectrochemical and biochemical assay comparison study of the healing effect of the Aloe vera and Hypericum perforatum loaded nanofiber dressings on diabetic wound. Spectrochim Acta A Mol Biomol Spectrosc .2021; 254: 119639.
21- Altan A, Aras MH, Damlar İ, Gökçe H, Özcan O, Alpaslan C. The effect of Hypericum Perforatum on wound healing of oral mucosa in diabetic rats. Eur Oral Res. 2018; 52(3): 143-149.
22- Uzunhisarcıklı E, Yerer MB. Role of Hypericum perforatum oil and pomegranate seed oil in wound healing: an in vitro study. Z Naturforsch C J Biosci. 2021; 77(5-6): 189-195.
23- Schemp CM, Pelz K, Wittmer A, Sch¨opf E, Simon JC. Antibacterial activity of hyperforin fromSt John’s wort, against multiresistant Staphylococcus aureus and gram-positive bacteria. The Lancet.1999.
24- Yalcınkaya E, Mert Basaran M, Tunckasık ME, Yazici GN, Elmas C, Kocaturk S. Efficiency of hypericum perforatum, povidone iodine, tincture benzoin and tretinoin on wound healing. Food Chem Toxicol. 2022; 166: 113209.
25- Thomson Healthcare. PDR for Herbal Medicines. 4th ed. Montvale; NJ, USA; 2007.
26- Demirhan I, Korkmaz A, Oner E, Gumuscu N, Erbil Y, Babaarslan O, et al. Synthesis, characterization, and antibacterial effect of St. John's wort oil loaded chitosan hydrogel. Int J Biol Macromol. 2024; 260(Pt 1): 129444.
27- Ponphaiboon J, Limmatvapirat S, Limmatvapirat C. Development and Evaluation of a Stable Oil-in-Water Emulsion with High Ostrich Oil Concentration for Skincare Applications. Molecules. 2024; 29(5): 982.
28- Khodabakhshi D, Vaseghi G, Mirzaee A, Eskandarinia A, Zargar Kharazi A. Antimicrobial activity and wound healing effect of a novel natural ointment: an in vitro and in vivo study. J Wound Care. 2023; 32(6): S18-S26. [In persian]
29- Liu X, Chen T, Liu X, Chen Y, Wang L. Penetration effect of ostrich oil as a promising vehicle on transdermal delivery of sinomenine. J Oleo Sci. 2013;62: 657-664.
دوره 8، پائیز
پاییز 1404
صفحه 24-39

  • تاریخ دریافت 16 اردیبهشت 1404
  • تاریخ بازنگری 11 تیر 1404
  • تاریخ پذیرش 12 تیر 1404