تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

اولین شناسایی مولکولی انگل خونی جنس Dactylosoma Labbé 1894 در وزغ تالشی (Bufo eichwaldi) از شمال ایران

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه زیست شناسی- دانشکده علوم پایه- دانشگاه گیلان- رشت- ایران
2 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران
چکیده
انگل‌های کوکسیدیایی (Apicomplexa: coccidian) از مهمترین انگل‌های خونی هستند که از خزندگان و دوزیستان گزارش شده‌اند. داکتیلوزوماتیده یکی از چهار خانواده‌ای است که متعلق به کوکسیدیاهای آدلورینید و شامل جنس‌های 1956Babesiosoma Jakowska and Nigrelli, و 1894،Labbe Dactylosoma می باشد. این پروتوزوئرهای خونی در خون محیطی مهره‌داران پست‌تر و اکثراً از دوزیستان گزارس شده‌است. وزغ تالشی (Bufo eichwaldi) یک دوزیست اندمیک بزرگ است که در جنگل‌های هیرکانی در شمال ایران و جنوب شرق آذربایجان پراکنش دارد. در مطالعه‌ی حاضر برای اولین بار شیوع و شناسایی ژنتیکی انگلهای خونی از جنس Dactylosoma در 75 فرد از B. eichwaldi به عنوان یک گونه‌ی با وضعیت حفاظتی آسیب پذیر از شمال ایران مورد بررسی قرارگرفت. آلودگی به انگل‌های خونی در 66/26 درصد از نمونه‌ها مشاهده گردید. نتایج تحلیل فیلوژنتیکی با استفاده از ژن s rRNA 18 نشان‌داد که 100 درصد مشابهت با Dactylosoma kermiti و D. ranarum وجود دارد. آنالیز فیلوژنتیکی نشان‌داد که داکتیلوزوما در مطالعه‌ی حاضر یک گروه مونوفایلتیک با گونه‌های داکتیلوزوما تشکیل داده و به عنوان گونه مشابه با Dactylosoma kermiti از وزغ Sclerophrys gutturalis و همچنین به عنوان کلاد خواهری با D. ranarum ، Dactylosoma sp و Babesiosoma stableri قرارگرفت (80%Support>). این اولین تعیین خصوصیت مولکولی از گونه Dactylosoma در وزغ‌های ایرانی است.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

First molecular identification of blood parasites of the genus Dactylosoma in Bufo eichwaldi from the north of Iran

نویسندگان English

Hossein Javanbakht 1
Fatemeh Abdolahi 2
1 Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
2 Department of Biology, Faculty of science, University of Guilan, Rasht, Iran
چکیده English

Coccidian parasites (Apicomplexa: coccidia) are among the most common blood parasites reported in reptiles and amphibians. The Dactylosomatidae Jakowska and Negrelli, 1955 are one of four families belonging to adeleorinid coccidia and comprise the genera Babesiosoma Jakowska and Nigrelli, 1956 and Dactylosoma Labbé, 1894. These blood protozoa occur in the peripheral blood of lower vertebrates and are commonly reported parasitising amphibians. Talysh bufo (Bufo eichwaldi) is a large endemic amphibian distributed in the Hyrcanian forest in the north of Iran and the southeast of Azerbaijan. In this study, we investigated for the first time the prevalence and genetic identification of blood parasites of the genus Dactylosoma in 75 individuals of B. eichwaldi with a vulnerable conservation status from north of Iran. Blood parasite infection with Dactylosoma sp was detected in 26.66% of samples. Results of phylogenetic analysis by 18S rRNA gene showed 100% identity with Dactylosoma kermiti and D. ranarum. Phylogenetic analysis showed the Dactylosoma of the present study formed a monophyletic group in Dactylosoma species, as a same species clustered with Dactylosoma kermiti from Sclerophrys gutturalis and as a sister group of D. ranarum, Dactylosoma sp, and Babesiosoma stableri (support > 80%). This is the first molecular characterization of a species of Dactylosoma from an Iranian Bufo.

کلیدواژه‌ها English

Amphibia
Apicomplexa
Haemogregarine
Phylogenetic
Hyrcanian
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دوره 8، شماره 4
زمستان
زمستان 1404 شناسه مقاله:10.22034/nfvm.2025.515051.1278

  • تاریخ دریافت 16 فروردین 1404
  • تاریخ بازنگری 17 آبان 1404
  • تاریخ پذیرش 15 آذر 1404