تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

بررسی بورخولدریا در خاک استان گلستان

نوع مقاله : مقاله پژوهشی

نویسنده
عضو هیات علمی موسسه تحقیقات واکسن و سرم سازی رازی
چکیده
بیماری ملیوئیدوز که عامل آن بورخولدریا سودومالئی است، یک بیماری مشترک انسان و دام با مخزن جوندگان و زیستگاه خاک است. کشاورزان به‌دلیل نوع کار، در معرض خطر عفونت با این بیماری و دیگر گونه­های پاتوژن بورخولدریا قرار دارند. اطلاعات کمی در مورد خواص فیزیکی و شیمیایی خاک مرتبط با حضور یا عدم وجود ارگانیسم در نقاط مختلف ایران وجود دارد. مقاومت ضد میکروبی همچنان چالش­های عمده­ای را برای مراقبت­های بهداشتی مدرن ایجاد می­­کند. این اولین مطالعه­ای است که حضور بورخولدریا را در نمونه­های خاک استان گلستان نشان می­دهد. تحقیق حاضر یک بررسی در استان گلستان است که ۱۰۰ نمونه‌ی خاک برای وجود این باکتری­ها غربال شدند. نمونه‌ها از منطقه‌ی برنج‌کاری استان گلستان جمع‌آوری و در محیط­های انتخابی اشدون کشت داده شدند. کلنی­های مشکوک بورخولدریا با آزمایش بیوشیمیایی و با استفاده از واکنش زنجیره­ای پلیمراز با استفاده از پرایمرهای اختصاصی R2-23CVP ،F1-23CVMP ، شناسایی شدند. از 100 نمونه‌ی خاک، 57 نمونه بورخولدریا جدا شد. حضور ارگانیسم نشان می­دهد که خاک می­تواند یک مخزن بالقوه برای ایجاد بیماری در انسان و دام باشد. این تحقیق، حضور بورخولدریا در خاک استان گلستان را تایید نمود و با یافته­های دیگر محققین که بورخولدریا سپاسیا را از بیماران سیستیک فیبروزیس مجزا نموده‌اند هم­خوانی داشت.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

The survey of Burkholderia in the soil of Golestan province

نویسنده English

parastoo poorghafour langeroodi
Faculty member of Vaccine and Serum Research Institute
چکیده English

Melioidosis, caused by Burkholderia pseudomallei, is a zoonotic disease (shared between humans and animals) with rodents as reservoirs and soil as its natural habitat. Farmers, due to the nature of their work, are at risk of infection with this disease as well as other pathogenic Burkholderia species. There is limited information about the physicochemical properties of soil associated with the presence or absence of this organism in different regions of Iran. Antimicrobial resistance continues to pose major challenges to modern healthcare.
This is the first study to demonstrate the presence of Burkholderia in soil samples from Golestan Province. The present study is a survey conducted in Golestan Province in which 100 soil samples were screened for the presence of these bacteria. Samples were collected from rice-growing areas of the province and cultured on selective Ashdown media. Suspected Burkholderia colonies were identified using biochemical tests and polymerase chain reaction (PCR) with specific primers CVMP23-1-F and CVP23-2-R.
Out of 100 soil samples, Burkholderia was isolated from 57 samples. The presence of this organism indicates that soil can serve as a potential reservoir for disease transmission to humans and animals. This study confirmed the presence of Burkholderia in the soil of Golestan Province and was consistent with findings from other researchers who have isolated Burkholderia cepacia from cystic fibrosis patients.

کلیدواژه‌ها English

Burkholderia
Soil
Golestan Province
1-Zoghi E. Human and animal transmissible disease Zoonotic.1993 Section A; 677-690 [In Persian].
2-Baharsefat M, Amjadi R. Equine melioidosis in Iran. Arch Razi Inst.1970; 22(1):209-213[In Persian]. doi:10.22092/ari.1970.108685.
3-Arefnejad M, Tajzade H, Barjesteh P. A history of Burkholderia-caused infections in Iran. J Paramede Sci Rehabil. 2013 2(2):54-59. doi:10.22038/jpsr.2013.1714. [In Persian].
4-Galimand M, Dodin A. Repartition de Pseudomonas pseudomallei en France et dans le monde la melioidose. Bull Soc Vet Prat de   France. 1982.66:651-657.
5-Baradaran-Seyed, Zahra. Is Melioidosis a One Health-Neglected Disease in Iran? Int J Travel Med Glob Health. 2020 Aug; 8(3):93-95 Doi: 10.34172/ijtmgh.2020.16
6-Pourtaghva M, Dodin A, Portovi M, Teherani M, Galimand M. 1st case of human pulmonary melioidosis in Iran. Bull Soc Pathol Exot Filiales. 1977; 70(2):107-109
7-Darazam IA, Kiani A, Ghasemi S, Sadeghi H, Alavi F, Moosavi MJ,et al ., Melioidosis: It is not Far From here. Tanaffos. 2011; 10(4):64-68.
8-Limmathurotsakul D, Dance DA. Global Burden and Challenges of Melioidosis. Basel: Multidisciplinary Digital Publishing Institute (MDPI); 2019
9-Soltan Dallal MM, Telefian CF, Hajia M, Kalantar E, Dolatyar Dehkharghani AR, Rahimi Forushani A, et al. Identification and molecular epidemiology of nosocomial outbreaks due to Burkholderia cepacia in cystic fibrosis patients of Masih Daneshvary Hospital, Iran. J Prev Med Hyg. 2014; 55(1):27-30.
10-Eram SM, Behzadian Nejad Q, Khatami GR, Nafissi N. Detection of Burkholderia cepacia complex in patients with cystic fibrosis. Tanaffos. 2004; 3(9):47-52.
11-Akbarein H, Bahonar AR, Dabbagh Moghaddam A, Bolouki Z, Hosseini Shokouh SJ. Glanders, a new vision on an old biological weapon. Ann Mil Health Sci Res. 2012; 10(2):143-162. [In Persian]
12-Dashtipour sh, Tadayon K, Mosavari, N Bidouki SK. A customized dual-locus VNTR combination for genotyping Burkholderia mallei field isolates in Iran. Veterinary Researches & Biological Products. 2018; Volume 31, Issue 2 May 2018 :51-58
13-Khaki P, Mosavari N, Khajeh Nasiri S, Emam M, Ahouran M, Hashemi S, et al. Glanders outbreak at Tehran Zoo, Iran.Iranian Journal of Microbiology.2012; Volume 4 Number 1 (March 2012) 3-7
14-Shojaat Dashtipour Sh, Tadayon K, Yazdansetad S, Mosavari N, Keshavarz R. Genomic pattern analysis of Burkholderia mallei field isolates by pulsed-field gel electrophoresis (PFGE) discriminatory typing Iran J. Microbiol 2021; Volume 13 Number 5 (October 2021) 574-582
15- Hantrakun V, Rongkard P, Oyuchua M, Amornchai P, Lim CH, Wuthiekanun V et al., Soil Nutrient Depletion Is Associated with the Presence of Burkholderia Pseudomallei. Appl Environ Microbiol. 2016; Nov 21; 82(24):7086-7092.
16-Jayasinghearachchi HS, Corea EM, JayaratneKl, Fonseka RA, Muthugama TA, Masakorala J,et al. Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. PLoS Negl Trop Dis. 2021; Dec 1; 15(12): e0009917. Doi: 10.1371/journal.pntd.0009917
17-Duangurai T, Indrawattana N, Pumirat P. Burkholderia pseudomallei Adaptation for Survival in Stressful Conditions.Review Biomed Res Int. 2018; May 27; 3039106. Doi: 10.1155/2018/3039106. E Collection 2018.
18-Herold R, Scholtysik R, Moroniak S, Weiss CH, Ishikawa H, Schroten H,et al ., Capsule-dependent impact of MAPK signalling on host cell invasion and immune response during infection of the choroid plexus epithelium by Neisseria meningitidis. Fluids Barriers CNS. 2021 Dec 4; 18(1):53. Doi: 10.1186/s12987-021-00288-7.
19-Lipuma JJ. Update on the Burkholderia cepacia complex. Current Opinion in Pulmonary Medicine 2005; 11(6): 528-33
20-Currie BJ, Dance DA, Cheng AC. The global distribution of Burkholderia pseudomallei and melioidosis: an update. Trans R Soc Trop Med Hyg. 2008; 102 Suppl 1: S1-4.
21-Holden MTG, Titball RW, Peacock SJ, Cerdeño- Tárraga AM, Crossman TALC, Pitt T,et al ., Genomic plasticity of the   causative agent of melioidosis, Burkholderia pseudomallei. Proceedings of the National Academy of Sciences of the United States of America (PNAS) 2004; 101(39):14240-50
22- Wuthiekanun V, Dance D. Standard Operating Procedure (SOP)Simplified method for the isolation of Burkholderia pseudomallei from soil 26nd June 2012; Version 1.12
23-Limmathurotsakul D, Dance DA, Wuthiekanun V, Kaestli M, Mayo M, Warner J, et al. Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei. PLoS Negl Trop Dis. 2013; 7: e2105. Pmid: 23556010
24-Shariful Alam Jilani MD, Mohammad Robayet JA, Mohiuddin MD, Hasan R, Rafiqul Ahsan CH, Ashraful Ha J.. Burkholderia pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh, PLoS Negl Trop Dis. 2016; Jan 15; 10(1): e0004301.
25- E. L Ong C, Wongsuvan G, S.W. Chew J, Yian Kim T, Teng LH, Amornchai P, et al. Presence of Burkholderia pseudomallei in Soil and Paddy Rice Water in a Rice Field in Northeast Thailand, but Not in Air and Rainwater, Am J Trop Med Hyg. 2017; Dec; 97(6):1702-1705.
26-Dance, D A. Ecology of Burkholderia pseudomallei and the interactions between environmental Burkholderia spp. and human-animal hosts Acta Trop, 2000 Feb 5; 74(2-3):159-68
27- Price EP, Sarovich DS, Smith EJ, MacHunter B, Harrington G, Theobald V, et al. Unprecedented Melioidosis Cases in Northern Australia Caused by an Asian Burkholderia pseudomallei Strain Identified by Using Large-Scale Comparative Genomics, Appl Environ Microbiol. 2015; Nov 25;82(3):954-63.
28-Palasatien S, Lertsirivorakul R. Soil physicochemical properties related to the presence of Burkholderia pseudomallei, Trans R Soc Trop Med Hyg.  2008; Dec;102 Suppl 1: S5-9.
29-Currie J B. Advances and remaining uncertainties in the epidemiology of Burkholderia pseudomallei and melioidosis, Trans R Soc Trop Med Hyg. 2008; Mar;102(3):225-7.
30-Near East and the Eastern neighbors of the boundary conditions Affairs. Available from; http://zahedaneh.ir [In Persian]
31-Hornstra H, Pearson T, Georglsa Sh, Liguorl A, Dale J, Price E, et al. Molecular epidemiology of Glanders, Pakistan. Emerg Infect Dis. 2009 Dec; 15(12): 2036–2039. 39.17
32-Alavi Darazam I, Kiani A, Ghasemi Sh, Sadeghi H, Alavi F, Moosavi MJ, et al. Melioidosis: It is not far from here. Tanaffos 2011; 10(4): 64-68.
33-Chetchotisakd P, Anunnatsiri S, Kiatchoosakum S, Kularbkaew C. Melioidosis pericarditis mimicking tuberculous pericarditis. Clinical Infectious Diseases 2010; 51(5): 46-49.
دوره 9، شماره 1
بهار 1405
صفحه 18-29

  • تاریخ دریافت 06 اسفند 1401
  • تاریخ بازنگری 23 اردیبهشت 1405
  • تاریخ پذیرش 19 اردیبهشت 1402