نوع مقاله : مقاله پژوهشی
نویسندگان
1 موسسه تحقیقات واکسن و سرم سازی رازی کرج، ایران
2 موسسه تحقیقات واکسن و سرم سازی رازی
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background and aim: The birds produce IgY immunoglobulin in their egg yolks, which is equivalent to the mammalian IgG. In this study, we investigated the quality and quantity of antibody in egg yolks of some domestic and ornamental birds.
Methods
Eggs of six different species of domestic and ornamental birds after disinfection and weighing of the yolk content were examined and the content of yolk fat was separated. The serum level was recorded and two consecutive levels of antibody precipitation with ammonium sulfate were followed and after dialysis, the antibody was extracted. At the final stage, the OD value were calculated and analyzed for each samples, separately.
Results: Estimated weight average of geese, ducks, native chicks, pigeons, quail and chicken eggs were 145.5, 62.7, 57.4, 19.5, 12.4 and g 1.6, and the weight percentage of yolk/ egg were 0.32, 0.31, 0.23, 0.16, 0.21 and 0.21, respectively. The serum levels were observed as 52 ml, 12.5 ml, 15 ml, 3.75 ml, 3 ml, 5.1 ml, respectively. The results of the concentration of antibody to each egg were 60450 μg / ml, 10631.25 μg / ml, 12026 μg / ml, 5203 μg / ml and 2535 μg / ml in OD280, respectively.
Conclusions: The results showed that the highest antibody production is related to geese and in next turn is for native chicken. Also, the use of quail egg as chicken egg alternative could be advisable.
کلیدواژهها [English]
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