بررسی اثر ضد میکروبی و ضد بیوفیلمی عطرمایه‌های نعناع فلفلی و آویشن شیرازی بر روی اسینتو باکتر بومانی و استافیلوکوکوس اورئوس مقاوم به آنتی‌بیوتیک‌های مختلف

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده پزشکی، گروه بیوشیمی، دانشگاه علوم پزشکی زابل

2 کارشناسی ارشد میکروبیولوژی، دانشگاه علوم پزشکی زابل

3 دانشجوی دکتری علوم دام، دانشگاه ملی زابل

4 گروه پژوهشی زراعت و اصلاح نباتات، پژوهشکده کشاورزی، دانشگاه زابل

5 پژوهشکده زیست‌فناوری کشاورزی، دانشگاه زابل

10.35066/J040.2019.697

چکیده

هدف از این مطالعه بررسی اثر ضد میکروبی و ضد بیوفیلمی نعناع فلفلی و آویشن شیرازی بر روی اسینتو باکتر بومانی و استافیلوکوکوس اورئوس مقاوم به آنتی‌بیوتیک‌های مختلف است. جدایه باکتری استافیلوکوکوس اورئوس و اسینتو باکتر بومانی از بیمارستان‌های شهرستان زابل جداسازی شد و عطرمایه گیاه آویشن شیرازی و نعناع فلفلی با دستگاه کلونجر به دست آمد. حداقل غلظت کشندگی و حداقل غلظت مهارکنندگی با روش میکرودایلوشن تعیین گردید. نتایج حاصل از بررسی نشان داد که کمترین غلظت مهارکنندگی عطرمایه نعناع فلفلی در برابر اسینتو باکتر بومانی و استافیلوکوکوس اورئوس برابر با 1.25 میلی‌گرم بر میلی‌لیتر بوده است. نتایج حاصل از مطالعه ما بر روی آویشن شیرازی نشان داد که کمترین غلظت عطرمایه آویشن در برابر اسینتو باکتر بومانی و استافیلوکوکوس اورئوس برابر با 25/1 و 31/0 میلی‌گرم بر میلی‌لیتر بوده است. نتایج نشان داد که عطرمایه نعناع فلفلی و آویشن شیرازی مهارکننده رشد باکتری‌های استافیلوکوکوس اورئوس و اسینتو باکتر بوده است.

کلیدواژه‌ها

عنوان مقاله [English]

Antimicrobial and anti-biofilm effects of Thyme essential oils and Peppermint on Acinetobacter baumannii and Staphylococcus aureus resistant to different antibiotics

نویسندگان [English]

  • fardin ali malayeri 1
  • zahra yazdanpour 2
  • hossein bandani 3
  • bahman fazeli nasab 4
  • saeide saeidi 5
1 دانشکده پزشکی، گروه بیوشیمی، دانشگاه علوم پزشکی زابل، زابل، ایران
2 کارشناسی ارشد میکروبیولوژی، دانشگاه علوم پزشکی زابل، زابل، ایران
3 دانشجوی دکتری علوم دام، دانشگاه ملی زابل، زابل، ایران
4 uoz
5 university of Zabol
چکیده [English]

The aim of this study was to investigate the antimicrobial and anti-biofilm effects of peppermint and Thyme on the antibiotic-resistant Acinetobacter baumannii and Staphylococcus aureus. Staphylococcus aureus and Acinetobacter baumannii strains were isolated from hospitals in Zabol city. The essential oil of Shirazi thyme and peppermint was obtained by Clevenger apparatus. Minimum lethal concentration and minimum inhibitory concentration were determined by microdilution method. Results showed that the lowest inhibitory concentration of peppermint essential oil against Acinetobacter baumannii and Staphylococcus aureus was 1.25 mg/ml. The results of our study on Thyme showed that the lowest concentration of Thyme essential oil against Acinetobacter baumannii and Staphylococcus aureus was 1.25 and 0.31 mg / ml, respectively. The results indicated that peppermint essential oil and Thymus vulgaris inhibited the growth of Staphylococcus aureus and Acinetobacter.



کلیدواژه‌ها [English]

  • Essential oil
  • Staphylococcus aureus
  • Acinetobacter baumannii
  • Minimum Inhibitory Concentration

مراجع

  1. Zarayneh S, Sepahi AA, Jonoobi M, Rasouli H. Comparative antibacterial effects of cellulose nanofiber, chitosan nanofiber, chitosan/cellulose combination and chitosan alone against bacterial contamination of Iranian banknotes. Int J Biol Macromol 2018; (118): 1045-1054. https://doi.org/10.1016/j.ijbiomac.2018.06.160
  2. Galeotti N, Mannelli LDC, Mazzanti G, et al. Menthol: a natural analgesic compound. Neurosci Lett 2002;322(3):145-148. https://doi.org/10.1016/S0304-3940(01)02527-7
  3. Fazeli-Nasab B, Mirzaei N. Evaluation of total phenol and flavonoid content in a wide range of local and imported plants. Scientific Journal of Ilam University of Medical Sciences 2018;26(2):141-154. http://doi.org/10.29252/sjimu.26.2.141 ]In Persian.[
  4. Davari A, Solouki M, Fazeli-Nasab B. Effects of jasmonic acid and titanium dioxide nanoparticles on process of changes of phytochemical and antioxidant in genotypes of Satureja hortensis L. Eco-Phytochemical Journal of Medicinal Plants 2018; 5(4): 1-20 ]In Persian[.
  5. Keikhaie KR, Fazeli-Nasab B, Jahantigh HR, Hassanshahian M. Antibacterial Activity of Ethyl Acetate and Methanol Extracts of Securigera securidaca, Withania sominefra, Rosmarinus officinalis and Aloe vera Plants against Important Human Pathogens. Journal of Medical Bacteriology 2018; 7(1-2): 13-21.
  6. Eteghad S, Mirzaei H, Pour S, Kahnamui S. Inhibitory effects of endemic Thymus vulgaris and Mentha piperita essential oils on Escherichia coli O157: H7. Research Journal of Biological Sciences 2009; 4(3): 340-344. Record Number : 20093100926
  7. Behnam S, Farzaneh M, Ahmadzadeh M, Tehrani AS. Composition and antifungal activity of essential oils of Mentha piperita and Lavendula angustifolia on post-harvest phytopathogens. Commun Agric Appl Biol Sci 2006; 71(3 Pt B): 1321-1326. PMID:17390896
  8. Yang S-A, Jeon S-K, Lee E-J, et al. Comparative study of the chemical composition and antioxidant activity of six essential oils and their components. Nat Prod Res 2010; 24(2): 140-151. https://doi.org/10.1080/14786410802496598
  9. Rasouli H, Farzaei MH, Mansouri K, et al. Plant cell cancer: may natural phenolic compounds prevent onset and development of plant cell malignancy? A literature review. Molecules 2016; 21(9): 1104. https://doi.org/10.3390/molecules21091104
  10. Mahboubi M, Haghi G. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. J Ethnopharmacol 2008; 119(2): 325-327. https://doi.org/10.1016/j.jep.2008.07.023
  11. Fazeli-Nasab B, Rossello JA, Mokhtarpour A. Effect of TiO2 nanoparticles in thyme under reduced irrigation conditions. Potravinárstvo: Slovak Journal of Food Sciences 2018; 12(1): 622-627. https://doi.org/10.5219/958
  12. Fazeli-nasab B, Moshtaghi N, Forouzandeh M. Effect of Solvent Extraction on Phenol, Flavonoids and Antioxidant Activity of some Iranian Native Herbs. Scientific Journal of Ilam University of Medical Sciences 2019; 27(3): 14-26. https:doi.org/10.29252/sjimu.27.3.14 ]In Persian[.
  13. Ghotaslou R, Salahi B. Effects of oxygen on in-vitro biofilm formation and antimicrobial resistance of Pseudomonas aeruginosae. Pharmaceutical Sciences 2013; 19(3): 96-99.
  14. Parsek MR, Greenberg E. Sociomicrobiology: the connections between quorum sensing and biofilms. Trends in microbiology 2005; 13(1):27-33. https://doi.org/10.1016/j.tim.2004.11.007
  15. Adal KA, Farr BM. Central venous catheter-related infections: a review. Nutrition 1996; 12(3): 208-213. https://doi.org/10.1016/S0899-9007(96)91126-0
  16. Shafiee P, SHOJA AS, Charkhabi AH. Biodegradation of polycyclic aromatic hydrocarbons by aerobic mixed bacterial culture isolated from hydrocarbon polluted soils. Iranian Journal Of Chemistry And Chemical Engineering(IJCCE) 2006; 25(3): 73-78 ]In Persian[.
  17. Prashanth K, Badrinath S. Simplified phenotypic tests for identification of Acinetobacter spp. and their antimicrobial susceptibility status. Journal of medical microbiology 2000; 49(9): 773-778. https://doi.org/10.1099/0022-1317-49-9-773
  18. Bauer A, Kirby W, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology 1966; 45(4): 493-496.
  19. Wikler MA. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. CLSI (NCCLS) 2006; 26(M7-A7). NII Article ID (NAID): 20001404762
  20. Owuama CI. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. African Journal of Microbiology Research 2017; 11(23): 977-980. https:doi.org/10.5897/AJMR2017.8545
  21. Lambert R, Pearson J. Susceptibility testing: accurate and reproducible minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values. J Appl Microbiol 2000; 88(5): 784-790. https://doi.org/10.1046/j.1365-2672.2000.01017.x
  22. Amiri A, Jomehpour N. Evaluation the Effect of Anti bacterial of Ferula assa-foetida L, Carum copticum, Mentha piperita L Hydroalcoholic Extract on Standard Sensitive and Methicillin- Resistant Staphylococcus aureus, Escherichia coli O157H7 and Salmonella typhimurium. journal of ilam university of medical sciences 2016; 24(2): 72-79. https:doi.org/10.18869/acadpub.sjimu.24.2.72 ]In Persian[.
  23. Jebelli Javan A, Ahmadi Hamedani M, Bayani M, et al. Antioxidant and antimicrobial effects of different mints, the most widely used in Caspian Sea areas, Iran. Journal of Veterinary Laboratory Research 2014; 6(2): 93-102. 10.22075/jvlr.2017.1265
  24. Kazem Alvandi R, Sharifan A, Aghazadeh Meshghi M. Study of chemical composition and antimicrobial activity of peppermint essential oil. Journal of Comparative Pathobiology 2010; 7(4): 355-364.
  25. Mahboubi M, Heidarytabar R, Mahdizadeh E. Antibacterial activity of Zataria multiflora essential oil and its main components against Pseudomonas aeruginosa. Herba Polonica 2017; 63(3): 18-24. https:doi.org/10.1515/hepo-2017-0015
  26. Dini S, Dadkhah A, Fatemi F. Biological Properties of Iranian Zataria Multiflora Essential Oils: A Comparative Approach. Electronic Journal of Biology 2015; 11(3): 57-62.
  27. Eftekhar F, Zamani S, Yousefzadi M, et al. Antibacterial activity of Zataria multiflora Boiss essential oil against extended spectrum β lactamase produced by urinary isolates of Klebsiella pneumonia. JJM 2011; 4(Supplement1): S43-S49.
  28. Moghtader M. In vitro antifungal effects of the essential oil of Mentha piperita L. and its comparison with synthetic menthol on Aspergillus niger. African Journal of Plant Science 2013; 7(11): 521-527. https:doi.org/10.5897/AJPS2013.1027
  29. Pramila D, Xavier R, Marimuthu K, et al. Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Mentha piperita: Lamiaceae). Journal of Medicinal Plants Research 2012; 6(2): 331-335. https:doi.org/10.5897/JMPR11.1232
  30. Aida A, Ali MS, Behrooz MV. Chemical composition and antimicrobial effect of the essential oil of Zataria multiflora Boiss endemic in Khorasan-Iran. Asian Pacific Journal of Tropical Disease 2015; 5(3): 181-185. https://doi.org/10.1016/S2222-1808(14)60649-6
  31. Mahboubi M, Kazempour N. Chemical composition and antimicrobial activity of peppermint (Mentha piperita L.) Essential oil. Songklanakarin J. Sci. Technol 2014; 36(1): 83-87.
  32. İşcan G, Ki̇ri̇mer N, Kürkcüoǧlu Mn, et al. Antimicrobial screening of Mentha piperita essential oils. Journal of agricultural and food chemistry 2002; 50(14): 3943-3946. https://doi.org/10.1021/jf011476k
  33. Mimica-Dukić N, Božin B, Soković M, et al. Antimicrobial and antioxidant activities of three Mentha species essential oils. Planta Med 2003; 69(05): 413-419. https:doi.org/10.1055/s-2003-39704
  34. Saharkhiz MJ, Motamedi M, Zomorodian K, et al. Chemical composition, antifungal and antibiofilm activities of the essential oil of Mentha piperita L. ISRN pharmaceutics 2012; 2012(Article ID 718645, 718646 Pages). https:doi.org/10.5402/2012/718645
  35. Karicheri R, Antony B. Antibacterial and antibiofilm activities of peppermint (mentha piperita linn) and menthol mint (mentha arvensis linn) essential oils on aggregatibacter actinomycetemcomitans isolated from orodental infections. European Journal Of Pharmaceutical And Medical Research 2016; 3(7): 577-580.
تازه ها در میکروب شناسی دامپزشکی
دوره 2، شماره 2
اسفند 1398
صفحه 41-51

فایل ها

سابقه مقاله

  • تاریخ دریافت: 18 آبان 1398
  • تاریخ بازنگری: 28 فروردین 1399
  • تاریخ پذیرش: 31 فروردین 1399

هم رسانی

ارجاع به این مقاله

آمار