تالاب میقان استان مرکزی، مرکز ورود ویروس آنفلوآنزای فوق حاد پرندگانH5، ایران، 1395

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری ویروس‌شناسی، گروه پاتوبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 استاد، گروه پاتوبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار، بخش بیماری‌های طیور، مؤسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

4 استاد، گروه میکروبیولوژی و ایمنولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

چکیده

طی دو دهه گذشته، ویروس آنفلوآنزای فوق حاد طیور سویه‌ H5 به دلیل ماهیت زئونوتیک بودن و ایجاد جهش فراوان مورد توجه بسیاری قرار گرفته است. هدف از مطالعه پیش رو بررسی خصوصیات فیلوژنتیک و مولکولی ژن هماگلوتینین (HA) سویه H5N8 وارد شده به ایران در سال 1395 در تالاب میقان شهرستان اراک استان مرکزی بود. بدین منظور نمونه‌های مربوطه به تخم‌مرغ‌های 14 -10 روزه تلقیح شده و پس از استخراج مایع آلانتوئیک و استخراج RNA و انجام PCR و تعیین توالی ژن‌ها، درخت‌های فیلوژنتیک توسط برنامه Mega7 رسم شده و خصوصیات مولکول شامل محل شکست Site) (Cleavage محل اتصال به گیرنده (Receptor binding Site)، محل گلیکوزیله (Glycosylation Site)، محل آنتی‌ژنیک (Antigenic Site) و جهش‌های مرتبط با ژن HA بررسی گردید. بر اساس آنالیز توالی اسید آمینه ژن HA، شامل محل شکست موتیف اسید آمینه پلی‌بازیک PLREKRRKR/GLF، که مشخصه ویروس‌های آنفلوآنزای فوق حاد بوده و جهش‌های T156A، S123P، S133A مرتبط با افزایش اتصال به اسید سیالیک پستانداران بود تجزیه و تحلیل فیلوژنیک ژن HA، بیانگر طبقه‌بندی این ویروس در کلد (Clade) b2.3.4.4 بوده و به نظر می‌رسد که ورود این سویه به ایران احتمالاً از طریق مسیر پروازی پرندگان وحشی مهاجر، از غرب آسیا به شرق آفریقا افتاده است.

کلیدواژه‌ها

عنوان مقاله [English]

Meighan Wetland of Markazi Province,Entrance Center of Avian Influenza Highly pathogenic virus H5 ,Iran ,2016

نویسندگان [English]

  • Minoo Motahhar 1
  • Hadi Keyvanfar 2
  • Abdolhamid Shoushtari 3
  • Mohammad Hossein Fallah Mehrabadi 3
  • Gholamreza Nikbakht Brujeni 4
1 PhD Student in Virology, Department of Pathobiology, Science and Research Branch, Islamic Azad University, Teh-ran, Iran
2 Professor, Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Assistant Professor, Department of Avian Diseases Research and Diagnostics, Razi Vaccine and Serum Research In-stitute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
4 Professor, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Teh-ran, Iran
چکیده [English]

During the past two decades, the highly pathogenic avian influenza H5N1 virus has received considerable attention due to its zoonotic and mutative features. Purpose of the leading study is molecular and phylogenetic characteristics of hemagglutinin (HA) gene of H5N8 strain identified in Meighan wetland of Arak city, Markazi province were investigated. For this purpose, samples were inoculated with embryonic eggs of 14-10 days and after extraction of allantoic fluid and RNA extraction and PCR and sequencing of genes, phylogenetic trees were drawn by Mega7 program and molecular properties including Cleavage site, Glycosylation site, Antigenic site, Receptor Binding site and HA gene-related mutations were investigated. Based on the analysis of the amino acid sequence of the HA genes, the cleavage site of the gene includes the PLREKRRKR / GLF polybasic amino acid motif, which is a characteristic of highly pathogenic influenza viruses. The HA gene of two viruses had T156A, S123P, S133A mutations associated with the increased mammalian sialic acid binding. Phylogenetic analysis of the HA gene of the virus studied in this study indicated the classification of this virus in the 2.3.4.4 b Clade. It seems that the introduction of these H5N8 HPAI strains in Iran probably occurred through the West Asia-East African flyway by wild migratory aquatic birds.

کلیدواژه‌ها [English]

  • Avian influenza
  • Highly pathogenic
  • Iran

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  • تاریخ دریافت: 25 تیر 1401
  • تاریخ پذیرش: 30 شهریور 1401

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