مقایسه اثرات آنتی‌باکتریال و هم‌افزایی برخی گیاهان دارویی علیه باکتری آئروموناس هیدروفیلا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه دامپزشکی، واحد شوشتر، دانشگاه آزاد اسلامی، شوشتر، ایران

2 گروه دامپزشکی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران

3 گروه پرستاری و مامایی، واحد بهبهان، دانشگاه آزاد اسلامی، بهبهان، ایران

چکیده

باکتری آئروموناس هیدروفیلا یک باکتری فرصت‌طلب است که با استفاده از گیاهان دارویی می‌توان موجب کاهش روند استفاده از داروهای شیمیایی و سنتزی جهت مهار رشد آن در مواد غذایی گردید. با توجه به خاصیت آنتی‌باکتریال سه گیاه بومادران، اسطوخودوس و آویشن، این مطالعه با هدف بررسی اثرات آنتی‌باکتریال و هم‌افزایی این سه گیاه علیه باکتری آئروموناس هیدروفیلا صورت گرفت. برای انجام این مطالعه ابتدا باکتری آئروموناس هیدروفیلا از نمونه‌های ماهی تهیه و با روش‌های بیوشیمیایی و PCR تأیید گردید. سپس عصاره‌گیری به روش ماسراسیون انجام شد و میزان حساسیت باکتری‌های جدا شده به روش انتشار از دیسک در محیط کشت مولر هینتون بررسی گردید. همچنین حداقل غلظت مهاری گیاهان دارویی مورد بررسی قرار گرفت. بر اساس نتایج به‌دست آمده میزان شیوع باکتری آئروموناس هیدروفیلا 5 درصد بود. باکتری‎های جدا شده نسبت به همه آنتی‌بیوتیک‌های مورد بررسی مقاومت داشتند. کمترین غلظت مهاری و کمترین غلظت کشندگی مربوط به گیاه بومادران بود که نشان از اثرات آنتی‌باکتریال قوی‌تر آن نسبت به دو گیاه دیگر دارد. بیشترین غلظت کشندگی برای گیاه اسطوخودوس گزارش شد که این اختلاف نسبت به دو گیاه دیگر از نظر آماری معنی‌دار (05/0>p) بود. به‌طور کلی ردیابی میزان شیوع این عوامل بیماری‌زا در آبزیان در کنار بررسی میزان شیوع مقاومت دارویی و بکارگیری درمان‌های جایگزین همچون استفاده از گیاهان دارویی دارای خواص ضد میکروبی که بتواند بر مقاومت‌های دارویی غلبه نماید و از شیوع بیشتر این عوامل در صنعت پرورش آبزیان جلوگیری نماید ضروری به نظر می‌رسد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparison of antibacterial and synergistic effects of some medicinal plants against the zoonotic bacterium (Aeromonas hydrophila)

نویسندگان [English]

  • Sanaz Hekmati 1
  • Seyedeh Ommolbanin Ghasemian 2
  • Hamid Mahmoodipour 3
1 Department of Veterinary, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran
2 Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran
3 Department of Nursing and Midwifery, Behbahan Branch, Islamic Azad University, Behbahan, Iran
چکیده [English]

Aeromonas hydrophila bacteria is an opportunistic bacterium that can reduce the use of chemical and synthetic drugs by using medicinal plants to inhibit its growth in food. Considering the antibacterial properties of three plants, Yarrow, Lavender and Thyme, this study was conducted with the aim of investigating the antibacterial and synergistic effects of these three plants against Aeromonas hydrophila bacteria. In this study, Aeromonas hydrophila bacteria was first prepared from fish samples and confirmed by biochemical and PCR methods. Then extraction was done by maceration method and the sensitivity of isolated bacteria was checked by disk diffusion method in Mueller Hinton culture medium. Also, the minimum inhibitory concentration of medicinal plants was investigated. According to the results, the prevalence of Aeromonas hydrophila bacteria was 5%. The isolated bacteria were resistant to all the investigated antibiotics. The lowest inhibitory concentration and the lowest lethal concentration were related to the yarrow plant, which shows its stronger antibacterial effects than the other two plants. The highest lethality concentration was reported for lavender plant, and this difference was statistically significant compared to other two plants (p<0.05). In general, tracking the prevalence of these pathogens in aquatic animals, along with investigating the prevalence of drug resistance and using alternative treatments such as the use of medicinal plants with antimicrobial properties that can overcome drug resistance and prevent the further spread of these factors in the aquaculture industry It seems necessary.

کلیدواژه‌ها [English]

  • Aeromonas hydrophila Lavender
  • Achillea Millefolium
  • Hydroalcoholic extract
  • Zataria multiflora Boiss
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