شناسایی مولکولی استافیلوکوکوس اورئوس در موارد ورم پستان تحت بالینی در مزارع گاو شیری شهرستان نیشابور

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکترای عمومی، گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

2 دانشیار گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

3 استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه زابل، زابل، ایران

چکیده

ورم پستان تحت بالینی یکی از معضلات شایع مزارع گاو شیری است که منجر به افت تولید و تحمیل خسارت اقتصادی به دامدار می‌شود. شناسایی پاتوژن‌های عامل ورم پستان تحت بالینی در هر منطقه برای پیشگیری و کنترل این معضل ضرورت دارد. هدف از مطالعه حاضر بررسی میزان شیوع مولکولی باکتری استافیلوکوکوس اورئوس در ورم پستان تحت بالینی در مزارع شیری شهرستان نیشابور بود. تعداد 500 رأس گاو شیری از 13 مزرعه واقع در نقاط مختلف شهرستان نیشابور وارد مطالعه شدند. نمونه شیر از تمام کارتیه‌ها گرفته شد و از نظر ابتلا به ورم پستان تحت بالینی با استفاده از آزمون ورم پستان کالیفرنیایی بررسی شدند. از کارتیه‌هایی که در آزمون ورم پستان کالیفرنیایی مثبت بودند مجدداً نمونه شیر اخذ شد و از نظر آلودگی به استافیلوکوکوس اورئوس با استفاده از واکنش زنجیره‌ای پلیمراز بررسی شدند. از مجموع 500 رأس گاو شیری مورد مطالعه (2000 کارتیه)، 94 (8/18 درصد) رأس مبتلا به ورم پستان تحت بالینی بودند که آلودگی به استافیلوکوکوس اورئوس در 8 رأس شناسایی گردید. بر اساس نتایج حاصل از این مطالعه میزان وقوع ورم پستان تحت بالینی و همچنین شیوع استافیلوکوکوس اورئوس در بین موراد ورم پستان تحت بالینی در مزارع شیری شهرستان نیشابور پایین است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Molecular identification of Staphylococcus aureus in cases of subclinical mastitis in dairy cattle farms of Neyshabur city

نویسندگان [English]

  • Ali Akbar Toosi 1
  • Mohsen Najimi 2
  • Mohammad javad Behzadi Shahrbabak 3
1 Graduated in doctor veterinary medicine, Department of pathobiology, Faculty of veterinary medicine, University of Zabol, Iran
2 Associate professor, Department of pathobiology, Faculty of veterinary medicine, University of Zabol, Zabol, Iran
3 Assistant professor, Department of clinical science, Faculty of veterinary medicine, University of Zabol, Zabol, Iran
چکیده [English]

Subclinical mastitis is one of the common problems of dairy farms, which leads to a decrease in production and economic damage to the farmers. Identification of the pathogens causing subclinical mastitis in each region is necessary to prevent and control this problem. The aim of this study was to investigate the molecular prevalence of Staphylococcus aureus bacteria in subclinical mastitis cows in dairy farms of Neyshabur city. A total of 500 milking cows from 13 dairy farms located in different parts of Neyshabur city were included in the study. Milk samples were taken from all quarters and checked for subclinical mastitis using the California mastitis test. Milk samples were taken from the quarters that were positive in the California mastitis test, and evaluated for contamination with Staphylococcus aureus using polymerase chain reaction. Out of 500 dairy cows studied (2000 quarters), 94 (18.8%) had subclinical mastitis, and Staphylococcus aureus infection was detected in 8 cows (8 quarter). Based on the results of this study, the occurrence rate of subclinical mastitis as well as the prevalence of Staphylococcus aureus among subclinical mastitis cows in dairy farms of Neyshabur city is low.

کلیدواژه‌ها [English]

  • Staphylococcus aureus
  • California mastitis test
  • Polymerase chain reaction
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