تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

پروفایل‌های مقاومت آنتی‌بیوتیکی و بیوتیپینگ استافیلوکوکوس اورئوس جداشده از لارو پشه: هشداری برای نظارت بر سلامت عمومی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه زابل، زابل، ایران
2 گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه بوعلی‌سینا، همدان، ایران
چکیده
تهدید فزاینده سویه‌های استافیلوکوکوس اورئوس مقاوم به آنتی‌بیوتیک یک چالش جدی برای سلامت عمومی جهانی است. هدف از انجام این مطالعه بررسی میزان مقاومت جدایه‌های استافیلوکوکوس اورئوس جداشده از لارو پشه کولکس در برکه‌های اب شهرستان زابل بود. تعداد 150 نمونه که هر نمونه حاوی 20 لارو پشه از 10 برکه در فصول بهار و تابستان از مناطق شهرستان زابل جمع‌آوری شد. نمونه‌ها به آزمایشگاه منتقل و شستشو داده شد سپس 5 سی‌سی PBS 1 درصد به آنها اضافه شد و توسط همزن هموژنیزه گردید. سپس رقت‌سازی سریالی انجام و در محیط‌های کشت بردپارکر و مانیتول سالت آگار کشت داده شد. کلنی‌های رشد کرده با استفاده از روش‌های بیوشیمیایی رایج میکروب‌شناسی تأیید هویت شد. بیوتیپینگ جدایه‌ها با استفاده از روش پیشنهادی Devriese انجام شد. مقاومت‌های آنتی‌بیوتیکی با استفاده از روش انتشار در دیسک انجام شد. بیشترین میزان مقاومت جدایه‌ها به آمیکاسین اگراسیلین و آمپی‌سیلین و بیشترین میزان حساسیت مربوط به سیپروفلوکساسین فورازولیدون و اریترومایسین بود. تجزیه و تحلیل بیوتیپینگ نشان داد که 40 درصد متعلق به اکوار انسانی، 20 درصد به اکوار مرغ و 5 درصد به اکوار گاوی بود. علاوه بر این، 10 ایزوله بیوتایپ‌های غیر اختصاصی (NHS) را نشان دادند، در حالی که 25 ایزوله با استفاده از زوش مورد مطالعه قابل تایپینگ نبودند. این مطالعه بر نقش بالقوه این ناقلین در انتشار پاتوژن‌های مقاوم به آنتی‌بیوتیک در جوامع تأکید و اهمیت این بیماری را برجسته می‌کند.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Antibiotic resistance profiles and biotyping of Staphylococcus aureus isolated from mosquito larvae: A warning for public health surveillance

نویسندگان English

Seyedeh Parisa Hasanein 1
Siamak Yari 2
1 Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
2 Department of Biology, Faculty of Basic Sciences, Bu Ali Sina University, Hamedan, Iran
چکیده English

The increasing threat of antibiotic-resistant strains of Staphylococcus aureus poses a serious challenge to global public health. The aim of this study was to assess the resistance levels of Staphylococcus aureus isolates obtained from mosquito larvae in the water pools of Zabol County. A total of 150 samples, each containing 20 mosquito larvae collected from 10 pools during spring and summer seasons in the Zabol County area, were gathered. The samples were transferred to the laboratory, washed, and then 5 ml of 1% PBS was added to them and homogenized using a homogenizer. Serial dilutions were made and cultured on Baird-Parker and Mannitol Salt Agar media. The grown colonies were identified using standard microbiological biochemical methods. The biotyping of isolates was performed using the Devriese proposed method. Antibiotic resistances were determined using the disk diffusion method. The results showed that 57 isolates of Staphylococcus aureus were identified. The highest resistance rates of isolates were to amikacin, oxacillin, and ampicillin, while the highest sensitivity rates were related to ciprofloxacin, fosfomycin, and erythromycin. Biotyping analysis indicated that 40% belonged to human strains, 20% to avian strains, and 5% to bovine strains. Additionally, 10 isolates showed non-specific biotypes (NHS), while 25 isolates were untypeable using the studied probe. These findings emphasize the critical need for continuous surveillance and management strategies to prevent the spread of antibiotic resistance in environmental reservoirs such as mosquito larvae. This study underscores the potential role of these vectors in the dissemination of antibiotic-resistant pathogens in communities and highlights the importance of this disease.

کلیدواژه‌ها English

antibiotic resistance
Staphylococcus aureus
mosquito larvae
public health surveillance
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دوره 7، شماره 1
بهار و تابستان
شهریور 1403
صفحه 46-53

  • تاریخ دریافت 19 فروردین 1403
  • تاریخ بازنگری 30 فروردین 1403
  • تاریخ پذیرش 22 اردیبهشت 1403