تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

بررسی آلودگی به لیستریا مونوسیتوژنز در پنیرهای سفید محلی ایرانی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 استاد، گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
2 کارشناسی ارشد، گروه میکروبیولوژی، دانشکده علوم، واحد زنجان، دانشگاه آزا د اسلامی، زنجان، ایران
3 دانشیار، گروه بهداشت مواد غذایی، واحد کرج، دانشگاه آزا د اسلامی، کرج، ایران
چکیده
لیستریا مونوسیتوژنز به‌عنوان یک باکتری گرم مثبت عامل ایجاد عفونت فرصت‌طلب بسیار کشنده در انسان است که از طریق طیف وسیعی از مواد خوراکی از جمله فراورده‌های لبنی، گوشتی و سبزیجات قابل انتقال به انسان است. هر چتد پنیر سفید محلی در کشور ایران تحت شرایط خوب تولید صورت نمی‌گیرد، اما تا کنون بررسی جامعی در باره شیوع سروتیپ‌های مختلف لیستریا مونوسیتوژنز در این فراورده در کشور انجام نشده است. به این منظور شهرهای بابل، قائم‌شهر، آمل، ارومیه، همدان، تهران و گرمسار به‌عنوان شهرهایی با مصرف بالای پنیر سفید محلی انتخاب شدند و در مجموع تعداد 343 نمونه پنیر محلی ایرانی از مراکز فروش این شهرها جمع‌آوری و به آزمایشگاه بهداشت و کنترل مواد غذایی منتقل گردید. جداسازی و شناسایی لیستریا مونوسیتوژنز در نمونه‌ها به کمک غنی‌سازی اولیه و کشت در محیط انتخابی انجام شده، گونه‌های جداسازی شده با روش‌های بیوشیمیایی مورد تأیید قرار گرفتند و سپس سروتیپ هر گونه تعیین گردید. در نهایت شمارش نمونه‌های مثبت با روش بیشترین تعداد احتمالی 5 لوله‌ای انجام شد. در مجموع 9 مورد از 343 نمونه تحت بررسی از نظر وجود لیستریا مونوسیتوژنز مثبت تشخیص داده شد. بیشترین موارد آلودگی از شهر ارومیه (3 مورد) جداسازی شد. با توجه به احتمال بقای این باکتری در طی فرآوری پنیر سفید محلی، به نظر می‌رسد نظارت بر نحوه تولید، نگهداری و عرضه این فراورده باید افزایش یابد و تا حد امکان تولید این محصول در کارگاه‌هایی انجام شود که تحت نظر نهادهای قانونی فعالیت می‌کنند.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Investigating the contamination of Listeria monocytogenes in Iranian local white cheese

نویسندگان English

Afshin Akhoondzadeh Basti 1
Bizhan Morradi 2
Zohreh Mashak 3
1 Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2 Graduated student, Department of Microbiology, Faculty of Science, Zanjan Branch,Islamic Azad University, Zanjan, Iran
3 Associate Professor, Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran
چکیده English

Listeria monocytogenes, as Gram-positive bacteria, is the cause of a opportunistic fatal infection in humans, which can be transmitted via a wide range of food products, including dairy, meat, and vegetables. Local white cheese in Iran is not produced under good manufacturing practices, however a comprehensive study about the prevalence of different serotypes of Listeria monocytogenes in this product has not been done yet. Babol, Qaem-shahr, Amol, Urmia, Hamedan, Tehran and Garmsar cities were selected as areas with high consumption of local white cheese, and 343 samples were collected from the dairy shops and delivered to the Food Safety and Hygiene Laboratory. Isolation and identification of Listeria monocytogenes, from cheese samples were carried out by pre-enrichment and cultivation in the selective media, the isolates were confirmed by biochemical methods, and then the serotype of each species was determined. Finally, the positive samples was enumerated using the five-tubes most probable number method. 9 cases of 343 samples were contaminated for Listeria monocytogenes. Most positive cases were isolated from Urmia city (3 cases). Considering the possibility of the survival of this organism during the processing of local white cheese, it seems that the monitoring of the manufacturing, storage and supply should be increased for this product and as much as possible, the production should be done in plants under the supervision of regulatory organizations.

کلیدواژه‌ها English

Iranian local white cheese
Listeria monocytogenes
Most Probable Number
Serotype
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دوره 7، شماره 2
پائیز و زمستان
اسفند 1403
صفحه 10-22

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