تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

تاثیر سطوح مختلف مکمل روی (اکسید روی، نانو اکسید روی و روی- متیونین) بر جمعیت پروتوزوآیی و نیتروژن آمونیاکی شکمبه بره‌های نر نژاد بلوچی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانشجوی دکتری تخصصی،گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران
2 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران
3 استادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه جیرفت، جیرفت، ایران
چکیده
عنصر روی برای سلامت و تولید نشخوارکنندگان ضروری می‌باشد. این آزمایش به‌منظور بررسی اثر سطوح مختلف مکمل روی بر جمعیت جنس‌های مختلف پروتوزآ و نیتروژن آمونیاکی شکمبه بر روی ۲۴راس بره نر نژاد بلوچی ( سن ۴-۳ ماه و میانگین وزن ۲± ۲۰ کیلوگرم) در قالب طرح کاملاً تصادفی (چهار تیمار و شش تکرار) به مدت ۷۰ روز (۱۰ روز عادت‌پذیری و ۶۰ روز آزمایش) انجام شد. تیمارهای آزمایشی شامل: ۱) جیره پایه بدون مکمل روی 2) جیره پایه با 100 میلی‌گرم روی به ازاء هر کیلوگرم ماده خشک خوراک به شکل اکسید روی، 3) جیره پایه با 25 میلی‌گرم روی به ازاء هر کیلوگرم ماده خشک خوراک به شکل نانو اکسید روی 4) جیره پایه با 50 میلی‌گرم روی به ازاء هر کیلوگرم ماده خشک خوراک به شکل روی- متیونین، بودند. در پایان دوره از مایع شکمبه برای اندازه‌گیری نیتروژن آمونیاکی و شمارش جمعیت پروتوزوآ استفاده شد. نتایج این پژوهش نشان داد که تیمارهای آزمایشی به‌طور معنی‌داری بر جمعیت پروتوزای شکمبه اثر گذاشت (05/0>P)، تعداد کل پروتوزوا و زیرگونه انتودینیوم و دیپلودینیوم در مایع شکمبه بره‌های تغذیه شده با جیره‌های حاوی مکمل‌های روی بیشتر از گروه شاهد بود. با وجود این، تعداد گونه‌های ایزوتریشا، افریواسکولکس، داسیتریشا و اپیدینیوم تحت تأثیر تیمارهای آزمایشی قرار نگرفتند. غلظت نیتروژن آمونیاکی مایع شکمبه به‌طور معنی‌داری) 0۱/0>P) تحت تأثیر افزودن مکمل‌های روی قرار گرفت و در همه تیمارها روند کاهشی داشت. به‌طور کلی می‌توان نتیجه‌گیری کرد که مکمل روی ممکن است سبب افزایش تعداد پروتوزوا و کاهش غلظت نیتروژن آمونیاکی مایع شکمبه بره‌های پرواری شود.
کلیدواژه‌ها

عنوان مقاله English

The effect of different levels of zinc supplementation (zinc oxide, nanozinc oxide, and zinc-methionine) on the ruminal protozoan population and ammonia nitrogen of Baluchi male lambs

نویسندگان English

Marzieh Hashemzaei 1
Ghasem Jalilvand 2
kamal Shojaeian 2
Amir Moosaee 3
Mostafa Yousef Elahi 2
1 Ph.D. Student, Department of Animal Science, Faculty of Agriculture, Zabol Uinversity, Zabol, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, Zabol Uinversity, Zabol, Iran
3 Assistant professor, Department of Animal Science, Faculty of Agriculture, Jiroft Uinversity, Jiroft, Iran
چکیده English

The zinc (Zn) element is essential for the health and production of ruminants. This experiment was conducted to investigate the effect of different levels of zinc supplementation on the population of different species of protozoa and rumen ammonia nitrogen (NH3-N) in 24 Baluchi male lambs (age of 3-4 months and average body weight of 20±2 kg) as a completely randomized design (four treatments and six replications each) for 70 days (10 days of adaptation and 60 days of data collection). The experimental treatments were 1) basal diet without Zn supplement (control), 2) basal diet supplemented with 100 mg of Zn/kg of feed’s dry matter as zinc oxide (ZnO), 3) basal diet supplemented with 25 mg of Zn/kg of dry matter as nano ZnO, and 4) basal diet supplemented with 50 mg of Zn/ kg of feed as zinc-methionine (ZnMet). At the end of the experiment, the ruminal fluid was used to measure the NH3-N and protozoan count.The results of this study indicated that the experimental treatments had a significant affected on the rumen protozoa population (P<0.05), the total number of protozoa and subspecies of Entodinium and Diplodinium count in the ruminal fluid of lambs fed diets containing zinc supplements was greater than that of the control group. However, the population of Isotricha, ophryoscolex, Dacytricha and Epidinium species were not affected by the experimental treatments. The NH3-N concentration of the ruminal fluid was significantly (P<0.01) affected by the dietary Zn supplementation and had a decreasing trend in all treatments. It can be concluded that Zn supplementation may increase the number of protozoa and decrease ammonia nitrogen concentration in the ruminal fluid of fattening lambs.

کلیدواژه‌ها English

Ammonia nitrogen
Lamb
Protozoa
Zinc supplement
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  • تاریخ دریافت 18 آبان 1403
  • تاریخ پذیرش 19 آبان 1403