تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

جداسازی و شناسایی مایکوپلاسما آگالاکتیه از گوسفند و بز در استان گلستان

نوع مقاله : مقاله پژوهشی

نویسنده
عضو هیات علمی، بخش تحقیقات بیماری‌های باکتریایی، مؤسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، ایران
چکیده
بیماری آگالاکسی یک بیماری عفونی و مسری در گوسفند و بز است که عامل آن مایکوپلاسما می‌باشد. اولین مطالعه بیماری آگالاکسی در استان گلستان است که با شناسائی کانون‌های بیماری و نمونه‌گیری از دام‌های مبتلا با روش کشت، سویه‌های دخیل در بیماری آگالاکسی جداسازی و سپس به روش PCR با استفاده از پرایمرهای اختصاصی، مایکوپلاسما آگالاکتیه مورد شناسایی و تائید قرار گرفت. در این پژوهش نمونه‌های اخذ شده شامل شیر، سوآپ از ضایعات چشم و واژن و پونکسیون از مایعات مفصلی گوسفند و بزهای مشکوک انجام شد. در آزمایش جداسازی بر روی نمونه‌های اخذ شده، جنس مایکوپلاسما در نمونه‌های بزی و گوسفندی به‌ترتیب 17/54 و 27/98 درصد تشخیص داده شد.  به‌طوری که جنس مایکوپلاسما در نمونه‌های شیر، سوآپ چشمی و سوآپ واژنی بزی به‌ترتیب 78/77، 85/42 و0درصد بود. همچنین در نمونه‌های شیر، سوآپ چشمی، سوآپ واژنی، مایع مفصلی و ترشحات جنین سقط شده گوسفندی به‌ترتیب 100 درصد،30/96، 100 ،100 و0درصد جنس مایکوپلاسما جدا شد. برای بررسی تأیید حضور جنس مایکوپلاسما و مایکوپلاسما آگالاکتیه با استفاده از روش PCR و تکثیر ژنSrRNA 16 باند اختصاصی جنس در bp163 و آغازگر FS2 جهت شناسائی گونه توانائی آغاز تکثیر قطعه‌ای از ژن لیپوپروتئین در bp375 انجام شد. تمامی نمونه‌های مثبت جنس جهت جداسازی گونه آگالاکتیه مورد ارزیابی قرار گرفتند. نمونه‌های مثبت با عامل مایکوپلاسما آگالاکتیه در بزها (92/76 درصد) و گوسفندان (57/24 درصد) به‌دست آمد. نتایج نشان می‌دهد که گونه‌های دیگر غیر از مایکوپلاسما آگالاکتیه در بزها (08/ 23 درصد) و گوسفندان (43/75 درصد) می‌تواند وجود داشته باشد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Isolation and identification of Mycoplasma agalactiae from sheep and goat, in Golestan province

نویسنده English

parastoo poorghafour langeroodi
Faculty Member, Department of Bacterial Diseases Research, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Iran
چکیده English

Contagious agalactia is a serious disease in sheep and goats caused by Mycoplasma. In this study, according of veterinary organization reports, the animals involved with contagious agalactia disease identification in Golestan province. Strains involved in disease identification by culture method, and then by PCR using specific primers to detect Mycoplasma agalactiae was confirmed. In this study, milk samples, swabs of lesions of the eye and ear and puncture of the joint fluid of sheep and goats were suspicious. The genus Mycoplasma in isolation test of sheep and goat samples were 54.17% and 98.72%, respectively. As of the genus Mycoplasma in milk, eye and ear swabs and joint fluid of sampling goat were 77.78%, 42.85%, and0%, respectively. In milk samples, eye and ear swabs and joint fluid of sheep were 100%, 96.30% 100% and 100%, respectively. To confirm the presence of Mycoplasma genus and Mycoplasma agalactiae, gene was amplified by using PCR. The SrRNA 16 primer was bands specific gender with bp163 and FS2 primer was ability to identify species by amplified fragment of the gene Lipoprotein with bp 375. All positive genus Mycoplasma samples were made to isolate Mycoplasma agalactiae. Positive samples caused by Mycoplasma agalactiae in goats and sheep were 76.92% and 24.57%, respectively. Results show that other species of Mycoplasma agalactiae in goats (23.08%) and sheep (75.43%) can exist.

کلیدواژه‌ها English

sheep and goat
golestan
Mycoplasma agalactiae
polymerase chain reaction
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دوره 8، بهار
بهار 1404
صفحه 1-10

  • تاریخ دریافت 11 بهمن 1401
  • تاریخ بازنگری 03 تیر 1402
  • تاریخ پذیرش 20 شهریور 1402