تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

تعیین هویت ژنتیکی واکسن‌های بیماری بورس عفونی پرندگان در ایران: کمک به انتخاب واکسن مناسب و مطالعات مولکولار اپیدمیولوژی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه میکروبیولوژی و ایمنی‌شناسی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
2 گروه علوم بالینی، دانشکده دامپزشکی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران
3 مؤسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران
چکیده
 
ویروس بیماری بورس عفونی یا گامبورو سبب بروز یک بیماری عفونی حاد و بسیار واگیردار شده که با سرکوب شدید سیستم ایمنی (به‌ویژه همورال) ماکیان شناخته می‌شود. این ویروس، یک RNA ویروس بسیار مقاوم از خانواده بیرناویریده (Birnaviridae) بوده که تنوع ژنتیکی آن به شکل قابل توجهی بر تظاهرات، تشخیص و کنترل بیماری اثرگذار می‌باشد. تقسیم‌بندی جدید ژنتیکی این ویروس بر اساس توالی نوکلئوتیدی قطعات (VP2)A و (VP1) B ژنوم آن صورت می‌گیرد. در سال‌های گذشته به علت برخی از مشکلات و محدودیت‌های واردات واکسن از کشورهای اروپایی، سازمان دامپزشکی کشور با واردات واکسن گامبورو و تولید آن در داخل کشور موافقت نمود. هدف این مطالعه بررسی شجره‌شناسی و تقسیم‌بندی سویه‌های واکسن و میزان مطابقت آنها با ویروس‌های در گردش می‌باشد. در این مطالعه، چهار نوع واکسن زنده تخفیف یافته (IBD07IR، مؤسسه واکسن وسرم‌سازی رازی، ایران)، B87 (QYH، چین)، ایمونوکمپلکس گامبوهچ (GUMBOHATCH ) (هیپرا، اسپانیا) و واکسن MyHatch UPM 93 (MVP، مالزی)  مورد ارزیابی قرار گرفت و سپس ژن‌های کدکننده دو پروتئین VP2 و VP1 با روش RT-PCR تکثیر و توالی یابی شد. به‌طور خلاصه واکسن‌های زنده تخفیف یافته IBD07IR و B87 ( اینترمدیت) و واکسن گامبوهچ (اینترمدیت پلاس) در گروه A1B1 و واکسن MyHatch UPM 93 (اینترمدیت پلاس) در گروه A3B2 قرار گرفتند. نتایج این مطالعه می‌تواند اطلاعات مناسبی را در حوزه واکسن‌های گامبورو و انتخاب واکسن در اختیار محققین و کلینیسین‌ها قرار داده و در مطالعات مولکولار اپیدمیولوژی در ایران مورد استفاده قرار گیرد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Genetic Characterization of Infectious Bursal Disease Virus Vaccines in Iran: Helping to make the right decision in choosing the right vaccine and mo-lecular epidemiology studies

نویسندگان English

Arash Ghalyanchi Langeroudi 1
Hossein Hosseini 2
Mohammad Abdoshah 3
Mohammad Hossein Fallah Mehrabadi 3
Ali Hojabr Rajeoni 1
Zahra Ziafati Kafi 1
Naser Sadri 1
Soroush Sarmadi 1
Fahimeh Jamiri 1
Alireza Bakhshi 1
Omid Eghbali 1
1 Department of microbiology and immunology, Faculty of veterinary medicine, University of Tehran, Tehran, Iran
2 Department of clinical science, Faculty of veterinary medicine, Karaj Branch, Islamic Azad university, Karaj, Iran
3 Razi Vaccine & Serum Research Institute, Agricultural Research, Education & Extension Organization, Karaj, Iran
چکیده English

Infectious bursal disease virus (IBDV) or Gumboro causes an acute and highly contagious infectious disease characterized by a severe suppression of the immune system (especially humoral) of chickens. This virus is a highly resistant RNA virus of the Birnaviridae family, whose genetic diversity has significant implications for disease manifestations, diagnosis and control. The new genetic classification of this virus is based on the nucleotide sequence of the A (VP2) and B (VP1) parts of its genome. Due to some problems and restrictions in importing vaccines from European countries, the Iranian veterinary organization has agreed in recent years to import Gumboro vaccine and produce it locally. The aim of this study is to investigate the phylogenetic and classification of vaccine strains and their compatibility with circulating viruses. In this study, four types of live attenuated vaccines (IBD07IR, Razi Vaccine and Serology Institute, Iran), B87 (QYH, China), GUMBOHATCH immunocomplex (Hypera, Spain) and MyHatch UPM 93 vaccine (MVP, Malaysia) were evaluated and then the genes encoding for two proteins VP2 and VP1 were amplified by RT-PCR method and sequenced. In brief, the live attenuated vaccines IBD07IR and B87 (Intermediate) and the Gumbohatch vaccine (Intermediate Plus) were categorized as group A1B1 and the MyHatch UPM 93 vaccine (Intermediate Plus) was classified as group A3B2. The results of this study can provide researchers and clinicians with appropriate information in the field of Gumboro vaccines and vaccine selection and can be used in molecular epidemiologic studies in Iran.

کلیدواژه‌ها English

Iran
Identification
Immune complex
Infectious bursal disease
Vaccine
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دوره 8، بهار
بهار 1404
صفحه 32-45

  • تاریخ دریافت 21 مرداد 1403
  • تاریخ پذیرش 08 شهریور 1403