تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

جداسازی و تعیین بایوار سویه‌های بروسلا در گاوهای شیری ایران

نوع مقاله : مقاله پژوهشی

نویسنده
دانشیار، مؤسسه تحیقات واکسن و سرم‌سازی رازی، سازمان تحقیات، آموزش و ترویج کشاورزی، کرج، ایران
چکیده
بروسلوز، یا تب مالت، یکی از بیماری‌های مشترک بین انسان و دام با گستره شیوع جهانی است. کنترل و پیشگیری از این بیماری نیازمند پایش و ارزیابی مستمر میزان آلودگی در جمعیت‌های دامی کشور می‌باشد، زیرا انتقال گونه‌های مختلف عامل بیماری از میزبان‌های اصلی به سایر دام‌ها، اهمیت بالای اپیدمیولوژیک دارد. از این رو، شناسایی گونه‌ها و بیووارهای غالب در مناطق مختلف جغرافیایی، گامی اساسی در برنامه‌های مبارزه با بروسلوز محسوب می‌شود. در این مطالعه، ۲۵۷ نمونه از غدد لنفاوی گاوهای راکتور مثبت متعلق به ۱۵ استان و ۴۲ دامداری راکتور مثبت سراسر کشور، به بخش بروسلوز مؤسسه تحقیقات واکسن و سرم‌سازی رازی ارجاع گردید. نمونه‌ها با روش‌های کشت میکروبی بررسی، و جدایه‌ها تعیین بیووار شدند. تأیید گونه‌ها و بیووارهای شناسایی‌شده، با آزمون مولتی‌پلکس انجام پذیرفت. نتایج نشان داد که در مجموع ۶۲ ایزوله بروسلا جدا گردید؛ از این میان، ۲۸ ایزوله Brucella melitensis بیووار ۱، پنج ایزوله B. melitensis بیووار ۳، و ۲۹ ایزوله Brucella abortus بیووار ۳ شناسایی شد. این یافته‌ها، با ترسیم پراکندگی انواع بیووار در نقاط مختلف ایران، بر تداوم خطر قابل توجه تب مالت برای سلامت عمومی جامعه تأکید دارد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Isolation and Biovar Determination of Brucella Strains in Dairy Cattle of Iran

نویسنده English

Saeed Alamian
Associate professor, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organi-zation (AREEO), Karaj, Iran
چکیده English

Brucellosis is a widespread zoonotic disease with a global distribution. The control and prevention of this disease require the assessment and monitoring of infection in the country’s livestock, as the transmission of this bacterial species from its primary host to other animals is of significant epidemiological importance. Therefore, determining the dominant species and biovars in various geographical areas is a key component of the strategy to combat this disease. In this regard, 257 lymph node samples from reactor-positive cattle, collected from 42 dairy farms across 15 provinces of Iran, were sent to the Brucellosis Department of the Razi Vaccine and Serum Research Institute. Samples were cultured, and the identity of the isolates was confirmed by multiplex PCR. In total, 62 Brucella isolates were recovered and identified as follows: 28 Brucella melitensis biovar 1, 5 Brucella melitensis biovar 3, and 29 Brucella abortus biovar 3. This study describes the prevalence of these biovars in different regions of Iran and emphasizes that brucellosis remains a significant risk to public health.

کلیدواژه‌ها English

Brucella melitensis
Brucella abortus
Diagnosis
Lymph Nodes
Positive Reactor
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دوره 8، پائیز
پاییز 1404
صفحه 1-16

  • تاریخ دریافت 02 مهر 1403
  • تاریخ بازنگری 08 آبان 1403
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