تازه ها در میکروب شناسی دامپزشکی

تازه ها در میکروب شناسی دامپزشکی

رخداد آفلاتوکسین M1 در انواع شیرهای عرضه‌شده در شهرستان شیراز به روش الایزا

نوع مقاله : مقاله پژوهشی

نویسندگان
امیرحسین هاشمی 1
نجمه واحد دهکردی 2
ابراهیم رحیمی 2
1 دانشگاه آزاد اسلامی واحد شهرکرد
2 گروه بهداشت مواد غذایی، گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
10.22034/nfvm.2026.580438.1312
چکیده
مایکوتوکسین‌ها متابولیت‌های ثانویه‌ای هستند که به طور طبیعی توسط کپک‌ها تولید می‌شوند و سبب آلودگی مواد غذایی در انسان و دام می‌شوند. آفلاتوکسین M1 یک ترکیب سرطان‌زای اصلی است که در شیر و فرآورده‌های لبنی یافت می‌شود و خطری دائمی برای مصرف‌کنندگان دارد. هدف از پژوهش رخداد آفلاتوکسین M1 در انواع شیرهای عرضه‌شده در شهرستان شیراز به روش الایزا است. 80 نمونه شیر شامل 20 نمونه شیر خام، 20 نمونه پاستوریزه، 20 نمونه ESL و 20 نمونه استریلیزه به‌صورت تصادفی در فصول بهار، تابستان، پائیز و زمستان نمونه‌گیری و جهت ارزیابی میزان آفلاتوکسین M1 به روش الایزا مطابق دستورالعمل کیت سنجش (Biopharm-R, Germany) در شرایط سرد به آزمایشگاه دانشگاه آزاد اسلامی شهرکرد انتقال داده شد. نتایج نشان داد تمام نمونه‌ها (100 درصد) به آفلاتوکسین M1 آلوده بودند، اما هیچ‌کدام از نمونه‌ها فراتر از استاندارد ایران نبود. به‌این‌ترتیب غلظت آلودگی در شیر خام 77/30، شیر پاستوریزه 45/32، شیر ESL 39/27 و شیر استریلیزه 24/24 نانوگرم بر لیتر بود. با توجه به آلودگی پائین‌تر از استاندارد ایران؛ اروپا و آمریکا در مطالعه حاضر، استفاده از انواع شیرهای عرضه شده درشهرستان شیراز نمی‌تواند برای سلامت مصرف‌کنندگان مخاطره‌ای جدی داشته باشد.
کلیدواژه‌ها
آفلاتوکسین M1
مایکوتوکسین
شیر
ایمنی غذایی
موضوعات

عنوان مقاله English

Occurrence of aflatoxin M1 in various types of milk supplied in Shiraz county by ELISA method

نویسندگان English

Amir Hossein Hashemi 1
Najmeh Vahed dehkordi 2
Ebrahim Rahimi 2
1 Islamic Azad University, Shahrekord Branch
2 Department of Food Hygiene, Shk.C., Islamic Azad University, ShahreKord, Iran
چکیده English

Mycotoxins are secondary metabolites naturally produced by molds and cause food contamination in humans and livestock. Aflatoxin M1 is a major carcinogenic compound found in milk and dairy products and poses a permanent risk to consumers. The aim of the study was to determine the occurrence of aflatoxin M1 in various types of milk supplied in Shiraz city using ELISA. 80 milk samples including 20 raw milk samples, 20 pasteurized samples, 20 ESL samples and 20 sterilized samples were randomly sampled in spring, summer, autumn and winter and were transferred to the laboratory of Islamic Azad University of Shahrekord under cold conditions for the evaluation of aflatoxin M1 levels by ELISA according to the instructions of the assay kit (Biopharm-R, Germany). The results showed that all samples (100%) were contaminated with aflatoxin M1, but none of the samples exceeded the Iranian standard. Accordingly, the concentration of contamination in raw milk was 30.77, pasteurized milk 32.45, ESL milk 27.39, and sterilized milk 24.24 ng/liter. Considering the contamination lower than the Iranian standard; Europe and America in the present study, the use of the types of milk supplied in Shiraz city cannot pose a serious risk to the health of consumers.

کلیدواژه‌ها English

aflatoxin M1
mycotoxin
milk
food safety
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  • تاریخ دریافت 14 اردیبهشت 1405
  • تاریخ بازنگری 19 خرداد 1405
  • تاریخ پذیرش 21 خرداد 1405